##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364251_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5255532 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.26542070336552 31.0 29.0 33.0 25.0 34.0 2 29.315491752309757 31.0 29.0 34.0 25.0 34.0 3 29.389274387445457 31.0 28.0 34.0 25.0 34.0 4 32.91107408346101 35.0 32.0 37.0 27.0 37.0 5 32.210298215290095 35.0 32.0 37.0 23.0 37.0 6 32.235126529531165 35.0 32.0 37.0 24.0 37.0 7 31.907626668432425 35.0 32.0 37.0 21.0 37.0 8 31.72580206913401 35.0 32.0 37.0 18.0 37.0 9 32.42354741632246 37.0 32.0 39.0 16.0 39.0 10 32.124917325210845 37.0 31.0 39.0 13.0 39.0 11 32.67345246875102 37.0 32.0 39.0 17.0 39.0 12 32.57656237275313 37.0 32.0 39.0 17.0 39.0 13 32.67988226501142 37.0 32.0 39.0 17.0 39.0 14 33.16558571044758 38.0 32.0 40.0 13.0 41.0 15 33.36931694070172 38.0 32.0 40.0 17.0 41.0 16 33.43340788335034 38.0 32.0 40.0 17.0 41.0 17 33.36453055561264 38.0 32.0 40.0 16.0 41.0 18 33.301963150447946 38.0 32.0 40.0 16.0 41.0 19 33.17591178209932 38.0 32.0 40.0 10.0 41.0 20 32.82916705673184 38.0 32.0 40.0 8.0 41.0 21 32.42983488636355 38.0 31.0 40.0 8.0 41.0 22 32.54817514192664 38.0 31.0 40.0 2.0 41.0 23 32.357085828799065 38.0 31.0 40.0 2.0 41.0 24 32.29861258574774 38.0 31.0 40.0 2.0 41.0 25 32.26042786914817 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6328715443075978 1101 2 0.600518638730744 1101 3 0.5936284793359974 1101 4 0.5274030283308235 1101 5 0.840036398283587 1101 6 0.7357591716649665 1101 7 1.0102096671816128 1101 8 1.0858661907338352 1101 9 1.9793044443938257 1101 10 2.023639244922368 1101 11 1.2353460392304498 1101 12 1.2180860621076732 1101 13 0.9643756837909763 1101 14 1.5887148099543964 1101 15 1.2022802281633957 1101 16 1.10625358182223 1101 17 1.1166308391755706 1101 18 1.226580057376772 1101 19 1.2312591377919588 1101 20 1.9513265879980182 1101 21 2.3049425715966905 1101 22 2.185590605155568 1101 23 2.0275788708798004 1101 24 1.8351697921733745 1101 25 1.9076235714791778 1102 1 0.2832746841619951 1102 2 0.4751424136576041 1102 3 0.46639557430224343 1102 4 0.3869478201184151 1102 5 0.6742121460575277 1102 6 0.48560685798720726 1102 7 0.74783358658118 1102 8 0.7356928234953095 1102 9 1.836955125303021 1102 10 1.8908522658010831 1102 11 1.063303677791552 1102 12 1.0510568536449227 1102 13 0.8164357821629764 1102 14 1.4160696510812585 1102 15 0.9186586069867104 1102 16 0.8504566857429481 1102 17 0.9487538470347161 1102 18 0.814462707795137 1102 19 0.7637108565203619 1102 20 1.3005400916823504 1102 21 1.9697898236493288 1102 22 1.8229504361253461 1102 23 1.6869182432097887 1102 24 1.5898351746698083 1102 25 1.5985504873106322 1103 1 0.25023159996401034 1103 2 0.37552148478124536 1103 3 0.4076660333090594 1103 4 0.3841853545202554 1103 5 0.6437857898206687 1103 6 0.4522213816788252 1103 7 0.6977275790467701 1103 8 0.6589278867998907 1103 9 1.5202775944769442 1103 10 1.5186678982357797 1103 11 0.8047918334518585 1103 12 0.8206904623640199 1103 13 0.6129117151092984 1103 14 1.3426548354830743 1103 15 0.8714073462469898 1103 16 0.7615929231929854 1103 17 0.6754263260979556 1103 18 0.41958501638053036 1103 19 0.37268968327882845 1103 20 1.1161882986522897 1103 21 1.704179500445214 1103 22 1.1653684593951041 1103 23 1.2090192244308682 1103 24 1.1137109382307742 1103 25 0.955390931696094 1104 1 0.12334374248864322 1104 2 0.36650051145928586 1104 3 0.12040033968485275 1104 4 0.1684120735152348 1104 5 0.3379268931422956 1104 6 0.16751082781534166 1104 7 0.3615839015237938 1104 8 0.4565518245915534 1104 9 1.2081551922201683 1104 10 1.2484210436149787 1104 11 0.6568430123486024 1104 12 0.40308673673415996 1104 13 0.389639753933686 1104 14 0.8781612875680409 1104 15 0.018357226110111924 1104 16 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2.2569179982389116 2203 25 2.472341372243619 2204 1 0.8168366745356117 2204 2 0.8310053131179593 2204 3 0.8350102373457418 2204 4 0.9110684670069062 2204 5 1.1475974815425474 2204 6 1.0590577498663336 2204 7 1.3197325613802846 2204 8 1.2053533582867964 2204 9 2.2390130439208775 2204 10 2.424027281981285 2204 11 1.475243579484463 2204 12 1.6702688343610461 2204 13 1.2585073982010542 2204 14 2.01291731573523 2204 15 1.7085371029934322 2204 16 1.663528872121475 2204 17 1.6267934640268749 2204 18 1.6249034941831724 2204 19 1.715044049665579 2204 20 2.413738412325941 2204 21 2.6635017772305716 2204 22 2.4944728036909396 2204 23 2.5074599923657246 2204 24 2.230431462653513 2204 25 2.4086945242888973 2205 1 0.4223890773254908 2205 2 0.5377415681251954 2205 3 0.5828613047178912 2205 4 0.503979149581518 2205 5 0.85369035609137 2205 6 0.45709037517503504 2205 7 0.3678823084670384 2205 8 0.9158268662927362 2205 9 1.508173493632583 2205 10 1.6157602713392585 2205 11 0.9552545638607128 2205 12 1.0484030966168802 2205 13 0.9718663467783131 2205 14 1.0304500320677406 2205 15 0.016675683267393993 2205 16 0.03715506935679258 2205 17 -0.007811797237614826 2205 18 0.5216909901671798 2205 19 0.5955706965539136 2205 20 1.2245660752478642 2205 21 1.3549279921367727 2205 22 0.7517125375612963 2205 23 -0.026117115300358762 2205 24 0.07100970655270444 2205 25 0.2730247228841449 2206 1 -1.2888650819929062 2206 2 -3.2699386489760833 2206 3 -1.0254897400946668 2206 4 -2.27827287297427 2206 5 -0.5425541092855006 2206 6 -0.9250025247421512 2206 7 -0.7931673775491923 2206 8 -0.07125076414457965 2206 9 1.2126112173518493 2206 10 0.7119215463949864 2206 11 0.4778891385551418 2206 12 0.15979890560203813 2206 13 0.037365402526262415 2206 14 0.6554526609198419 2206 15 -0.017652144155931637 2206 16 0.38027707513378317 2206 17 0.5587372971485323 2206 18 -0.15755600539088732 2206 19 0.3161706425062931 2206 20 0.8962637982112867 2206 21 1.37783818121963 2206 22 1.3305155219765084 2206 23 0.9682010458535757 2206 24 0.7990759482492891 2206 25 0.6314455014576339 2207 1 0.3779445558185017 2207 2 0.4263801663016622 2207 3 0.27605955150649564 2207 4 0.3444090039672716 2207 5 0.6974486982622139 2207 6 0.519355290768793 2207 7 0.424978843968681 2207 8 0.664168654952416 2207 9 1.2069554274174124 2207 10 1.5743638323078848 2207 11 0.3217225392083094 2207 12 0.6168329916049586 2207 13 0.6358076183290677 2207 14 1.5503677495002464 2207 15 0.9502515215880578 2207 16 0.03669782357161466 2207 17 0.2579338684819774 2207 18 0.6912650760856067 2207 19 0.9303752501699734 2207 20 1.4334161942119579 2207 21 1.6652859710107037 2207 22 1.5208606349846292 2207 23 0.7158392710086829 2207 24 1.1182430733432227 2207 25 0.9615307999761171 2208 1 0.20937822664484074 2208 2 0.39258252542872896 2208 3 0.4074247701478235 2208 4 0.41662384318004797 2208 5 0.48774028984485085 2208 6 0.47108710031188394 2208 7 0.8330914894161694 2208 8 0.8108693861598617 2208 9 1.7108325438791283 2208 10 1.8203170150449814 2208 11 0.9769942867341257 2208 12 1.034661204498974 2208 13 0.8381134905129102 2208 14 1.5940987131127216 2208 15 1.215647240767403 2208 16 1.0571893740228973 2208 17 0.9090092025563052 2208 18 0.682951970673173 2208 19 1.0716312412551758 2208 20 1.782278419678562 2208 21 2.378175501007391 2208 22 2.0526032091627826 2208 23 2.071807074177485 2208 24 1.7534608004004255 2208 25 1.5933074394098767 2209 1 0.3411866836110029 2209 2 0.30332357168247626 2209 3 0.31030659481287515 2209 4 0.2685619419283398 2209 5 0.4992437354997179 2209 6 0.25971442554583746 2209 7 0.39800788379115914 2209 8 0.3589443075797263 2209 9 1.2317520108276412 2209 10 1.2730456424138872 2209 11 0.26070606889800985 2209 12 0.7615344127267605 2209 13 0.42922647575178985 2209 14 0.8897882802993493 2209 15 0.5438030912852767 2209 16 0.3405756430330342 2209 17 0.004332182379840788 2209 18 0.18289378802731449 2209 19 0.1407122815812869 2209 20 1.0242338924934202 2209 21 1.6324451818042078 2209 22 1.497821496022972 2209 23 1.236728321133704 2209 24 0.6670132560875501 2209 25 0.6662017476131155 2210 1 0.01586168691123646 2210 2 0.2988440047024312 2210 3 0.4358697818885915 2210 4 0.4754013587267636 2210 5 0.15541246047060753 2210 6 0.44349986629980975 2210 7 0.5201520353418942 2210 8 0.5885932997193173 2210 9 1.680043956433451 2210 10 1.6042768326164598 2210 11 0.8130455598324389 2210 12 0.7590499977322693 2210 13 0.6544166375103089 2210 14 1.2491855774416862 2210 15 0.7150033693971594 2210 16 0.7981926490042213 2210 17 0.6361218573058665 2210 18 0.6602055919181709 2210 19 0.818560375110664 2210 20 1.517389443341422 2210 21 1.8242043863575006 2210 22 1.6202712816073372 2210 23 1.7156176156310181 2210 24 1.6799160366710666 2210 25 1.5020176569289205 2211 1 0.2991806592036461 2211 2 0.230458215431252 2211 3 0.1437353661165055 2211 4 0.19648142581930728 2211 5 0.3570604151891885 2211 6 0.1200530102719739 2211 7 0.5073861116382758 2211 8 0.4492060567401097 2211 9 1.470979047542201 2211 10 1.2706558583506897 2211 11 0.6754770338870273 2211 12 0.13790251372948603 2211 13 -0.07589735906630324 2211 14 0.6713225120594473 2211 15 0.43972672589542583 2211 16 0.45822283401619046 2211 17 0.21552791368776525 2211 18 0.032692532654280626 2211 19 0.1188244771170659 2211 20 0.8458285834077302 2211 21 1.7140740623727169 2211 22 1.5670879543929388 2211 23 1.4895508528907087 2211 24 1.3183869629961507 2211 25 1.0714022539779862 2212 1 0.8749310641359607 2212 2 0.8617035691453943 2212 3 0.7033138169002875 2212 4 1.062632969150414 2212 5 0.9743457285336774 2212 6 1.032963473304612 2212 7 1.4307355700465578 2212 8 1.4445635218417188 2212 9 -9.155309998146091 2212 10 -1.0116163770668116 2212 11 0.4267913479618528 2212 12 1.40997344936671 2212 13 1.3507105772397452 2212 14 1.9989755556805733 2212 15 1.6379704555223498 2212 16 1.5312085179304802 2212 17 1.605828376982501 2212 18 1.8271704493451537 2212 19 2.003448168195966 2212 20 2.6483405240627746 2212 21 2.8797677521896787 2212 22 2.7863453941000884 2212 23 2.7650465226036545 2212 24 2.5602884879148746 2212 25 2.62796200805456 2213 1 0.4414909898480843 2213 2 0.44588378698993836 2213 3 0.21205370266489965 2213 4 0.5056570785198886 2213 5 0.5062921132565279 2213 6 0.1703198841554041 2213 7 0.5219074571732989 2213 8 0.7817886091798876 2213 9 1.4151957871315872 2213 10 -8.587436046395506 2213 11 -2.4595453482927816 2213 12 -0.6757711286235875 2213 13 0.1313714206779082 2213 14 0.6968397621767934 2213 15 0.4190601261297502 2213 16 0.8498274465685824 2213 17 0.5347635863855515 2213 18 0.5219215774418231 2213 19 0.8778995908046667 2213 20 1.6096801777461565 2213 21 1.533037266603806 2213 22 1.8366695488054106 2213 23 1.4713100831837593 2213 24 1.4626389770701707 2213 25 1.1142138107185033 2214 1 -2.15263166889212 2214 2 -2.3780107215316697 2214 3 -2.223792998602267 2214 4 -2.223975803467045 2214 5 -2.1716687125047898 2214 6 -2.2485997717527546 2214 7 -10.279699028029327 2214 8 -3.9946488592983833 2214 9 -3.030013863212023 2214 10 -2.6660947830999966 2214 11 -3.070424587175065 2214 12 -11.945740397249551 2214 13 -5.470347269231521 2214 14 -13.395774860248824 2214 15 -7.012410960986287 2214 16 -5.0288654317864285 2214 17 -4.235463688904641 2214 18 -3.7578471809258964 2214 19 -3.8481116708848617 2214 20 -4.040110043587394 2214 21 -12.778178557234607 2214 22 -15.31384846713695 2214 23 -9.680785234238321 2214 24 -8.415942931199226 2214 25 -15.885974930780597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 185783.0 3 9.0 4 2951.0 5 27677.0 6 34551.0 7 27812.0 8 22643.0 9 18491.0 10 16154.0 11 14806.0 12 14169.0 13 13538.0 14 13536.0 15 13800.0 16 14505.0 17 15586.0 18 17824.0 19 21350.0 20 27542.0 21 36018.0 22 45628.0 23 55076.0 24 64271.0 25 74595.0 26 85768.0 27 97045.0 28 108589.0 29 128611.0 30 153149.0 31 186510.0 32 227678.0 33 280215.0 34 338121.0 35 402513.0 36 493800.0 37 658284.0 38 1316934.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.840704595956474 19.961477519829845 13.845691201591146 24.352126682622536 2 15.681310249493036 21.909653925085102 38.31949586817122 24.089539957250643 3 17.792896031686684 27.01000122349018 30.21449894842637 24.982603796396774 4 12.393112628750048 18.140637332243433 37.240606659801514 32.225643379204996 5 13.10028877492149 38.9207741359967 34.11642789836706 13.862509190714754 6 30.265771975541554 37.805228672357906 18.3406352839769 13.588364068123632 7 27.277660641032398 33.18363593430186 22.20915229423854 17.329551130427205 8 26.500786220392996 34.27233684499084 20.48991239258478 18.73696454203138 9 26.785158719995067 15.584858677808771 20.240364845818316 37.389617756377845 10 14.957010515940409 30.30468921699866 32.36772777481255 22.37057249224838 11 33.81082009874233 23.831930240249164 23.569223719928896 18.788025941079606 12 23.1198094387058 26.99118513201751 29.90355098899911 19.985454440277575 13 28.137818835684858 22.12406168834258 26.857928436194804 22.880191039777756 14 22.11463265103418 21.51462876936127 28.405435407630836 27.96530317197371 15 23.940666597913584 29.410094753670517 24.15409925027428 22.49513939814161 16 22.87287279668583 28.72652075200571 26.064905055511456 22.335701395797006 17 22.18107511912987 28.588542944122796 27.122317449160377 22.108064487586958 18 21.56525915323905 27.226017427202404 29.34221577475016 21.86650764480839 19 23.06019584184765 26.827128782540804 28.99369653437831 21.118978841233233 20 23.382472651486665 26.414538067184264 28.618369594238334 21.584619687090743 21 24.028933499691313 25.639866690316094 28.220077449333264 22.111122360659333 22 23.373801146043586 26.154953937329346 28.251841108191 22.219403808436073 23 22.79350745213528 26.135517483969295 28.49618968040584 22.574785383489584 24 22.654828695409773 26.424452539813775 28.46633132936472 22.454387435411732 25 22.711177023063094 26.472097019957776 28.62232223181218 22.19440372516695 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2160.0 1 2160.0 2 2986.5 3 3813.0 4 3813.0 5 3813.0 6 8950.5 7 14088.0 8 14088.0 9 14088.0 10 15545.0 11 17002.0 12 17002.0 13 17002.0 14 21360.0 15 25718.0 16 25718.0 17 25718.0 18 48741.0 19 71764.0 20 71764.0 21 71764.0 22 108266.5 23 144769.0 24 144769.0 25 144769.0 26 207739.5 27 270710.0 28 270710.0 29 270710.0 30 321204.0 31 371698.0 32 371698.0 33 371698.0 34 425182.5 35 478667.0 36 478667.0 37 478667.0 38 514595.0 39 550523.0 40 550523.0 41 550523.0 42 587321.0 43 624119.0 44 624119.0 45 624119.0 46 650833.5 47 677548.0 48 677548.0 49 677548.0 50 654549.0 51 631550.0 52 631550.0 53 631550.0 54 571463.0 55 511376.0 56 511376.0 57 511376.0 58 441553.0 59 371730.0 60 371730.0 61 371730.0 62 305800.5 63 239871.0 64 239871.0 65 239871.0 66 185015.5 67 130160.0 68 130160.0 69 130160.0 70 97803.5 71 65447.0 72 65447.0 73 65447.0 74 46974.0 75 28501.0 76 28501.0 77 28501.0 78 21835.0 79 15169.0 80 15169.0 81 15169.0 82 10562.5 83 5956.0 84 5956.0 85 5956.0 86 4136.0 87 2316.0 88 2316.0 89 2316.0 90 1558.5 91 801.0 92 801.0 93 801.0 94 437.0 95 73.0 96 73.0 97 73.0 98 38.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027723168653525466 2 0.013623739708939077 3 0.0014270677069419422 4 0.0 5 0.0043382858291035045 6 0.0016553985400526532 7 7.801303464615952E-4 8 0.048558357174877824 9 0.005156471314416885 10 0.0033678797883829837 11 0.00452856152336243 12 0.00608882221628562 13 0.0015602606929231903 14 0.0012558195821089093 15 1.9027569425892563E-4 16 7.611027770357025E-4 17 0.0025306667336437113 18 0.0013319298598124794 19 0.003215659232975843 20 0.8686275718614215 21 0.019027569425892562 22 0.007782275895190058 23 0.008771709505336471 24 0.0024165013170883557 25 6.849924993321323E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5255532.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.79719925385404 #Duplication Level Percentage of deduplicated Percentage of total 1 67.8897518736394 25.660424788626585 2 14.601651599668855 11.038030698960803 3 6.3464031799793625 7.1962879661691845 4 3.343834180852609 5.055502672221354 5 1.9534455635878665 3.6917385599243895 6 1.245354645917734 2.8242550616079254 7 0.8603727096061263 2.2763775116292737 8 0.612944872694546 1.8534079587891157 9 0.4447181049096663 1.5128188940760319 >10 2.353666081440526 16.121905744054033 >50 0.19425059285053933 5.117213758660143 >100 0.1384703841439814 10.256713968081444 >500 0.011065092661957278 2.9630770150309473 >1k 0.003705761299067368 2.3748723283213287 >5k 1.565814633408153E-4 0.4533646307349918 >10k+ 2.087752844544204E-4 1.60400844311257 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 29530 0.5618841251466073 No Hit TATCAACGCAGAGTACTTTTTTTTT 22702 0.431963881106613 No Hit GGTATCAACGCAGAGTACTTTTTTT 18662 0.35509250062600706 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 10413 0.19813408043181926 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 9784 0.18616573926293284 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 7278 0.13848265028164608 No Hit GTGGTATCAACGCAGAGTACATGGG 5919 0.11262418343185808 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.8055138851785126E-5 0.0 2 1.9027569425892563E-5 0.0 0.0 3.8055138851785126E-5 0.0 3 1.9027569425892563E-5 0.0 0.0 3.8055138851785126E-5 0.0 4 1.9027569425892563E-5 0.0 0.0 3.8055138851785126E-5 0.0 5 1.9027569425892563E-5 0.0 0.0 3.8055138851785126E-5 0.0 6 1.9027569425892563E-5 0.0 0.0 3.8055138851785126E-5 0.0 7 1.9027569425892563E-5 0.0 0.0 3.8055138851785126E-5 0.0 8 1.9027569425892563E-5 0.0 0.0 3.8055138851785126E-5 0.0 9 1.9027569425892563E-5 0.0 0.0 3.8055138851785126E-5 0.0 10 1.9027569425892563E-5 0.0 0.0 5.708270827767769E-5 0.0 11 3.8055138851785126E-5 1.9027569425892563E-5 0.0 5.708270827767769E-5 0.0 12 3.8055138851785126E-5 1.9027569425892563E-5 0.0 5.708270827767769E-5 0.0 13 3.8055138851785126E-5 1.9027569425892563E-5 0.0 7.611027770357025E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6995 0.0 13.288561 1 ACGGCCC 450 0.0 12.527973 15 TATCACG 205 0.0 12.020761 2 TATAACG 320 0.0 11.847384 2 TAACGCA 305 0.0 11.496147 4 CGCGCCA 235 0.0 11.285734 10 GCCCCCG 515 0.0 10.946669 18 GGTTGCG 80 3.8600233E-4 10.6614275 7 TCGCGGT 190 7.2759576E-12 10.5610485 17 CGGCCCC 525 0.0 10.556258 16 TGCACGG 590 0.0 10.274467 12 GTATCAA 14770 0.0 10.200309 1 CCGAGTA 280 0.0 10.148812 9 GTGGTAT 2130 0.0 10.142661 1 ACGCCGA 285 0.0 9.975604 6 TGGTATC 2155 0.0 9.851746 2 TGACCGA 485 0.0 9.653464 19 CCTATAA 955 0.0 9.624208 1 AACTGGC 860 0.0 9.555235 17 ATAACGC 370 0.0 9.476464 3 >>END_MODULE