FastQCFastQC Report
Thu 26 May 2016
SRR1364250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414318
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN50270.20821615048224798No Hit
GTATCAACGCAGAGTACTTTTTTTT45040.18655371827572012No Hit
TATCAACGCAGAGTACTTTTTTTTT32930.1363946257286737No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26820.11108727185068412No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA26060.1079393849526036No Hit
GGTATCAACGCAGAGTACTTTTTTT25000.10354891112107022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG653.367746E-614.61799118
GGTATCA24150.013.25826551
CGCGCCA655.4670716E-513.15072510
TATCACG1001.4415127E-712.3514322
CGACCGT701.0895647E-412.21646419
GGGCGCG802.8730306E-511.8729237
GGACCGT1001.9353884E-611.3977696
ATAACGC1850.011.3002513
TAATACC2550.011.1793934
CATGGTA9600.011.0862314
ACATGGT13600.011.0396513
TAACGCA1900.011.0028764
GTATCAA60450.010.8763641
TACATGT17050.010.6991872
CTCAACG1803.45608E-1110.5583154
TATAACG1803.45608E-1110.556782
TAGATAG2250.010.5534915
ACGCCCC1201.521048E-610.29350217
TCGCACG1303.8342841E-710.23259418
ACGGCCC1502.438901E-810.13535115
Produced by FastQC (version 0.11.5)