FastQCFastQC Report
Thu 26 May 2016
SRR1364250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414318
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT159370.6601035986145984No Hit
TATCAACGCAGAGTACTTTTTTTTT129820.5377087856694934No Hit
GGTATCAACGCAGAGTACTTTTTTT101060.4185861183158142No Hit
GTACTTTTTTTTTTTTTTTTTTTTT85060.3523148151983293No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51470.21318649821605937No Hit
GAGTACTTTTTTTTTTTTTTTTTTT50250.20813331135335114No Hit
GTGGTATCAACGCAGAGTACATGGG33180.13743011483988438No Hit
GGTATCAACGCAGAGTACATGGGAT27720.11481503265104265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT852.5400914E-713.47962719
TATAACG3000.013.2731032
ATAACGC3050.012.7428183
GGTATCA41800.012.6996921
CGTAAGA604.1639764E-412.6410512
ATCACGC2250.012.6392153
TATCACG2200.012.4974022
CGAAATC1551.8189894E-1212.22516613
AACGCCG1251.4606485E-912.1341515
TAACGCA2900.012.0949244
CCGTAAG550.00311956112.0647071
GGACCGA1052.7990245E-711.7366596
AGGACCG1157.291601E-811.5406345
AGTCCGG752.1259209E-411.37011110
TCACGCA2350.011.2950894
GTATTAG2350.011.29462051
CCGATTA600.005657874511.14011117
CGTAGTC600.005657874511.14011115
GTTCGAC951.2859948E-511.05635116
TCGACCG951.2862925E-511.0561218
Produced by FastQC (version 0.11.5)