FastQCFastQC Report
Thu 26 May 2016
SRR1364249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4528391
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN98940.21848820033429092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27650.012.6463581
TTGACCG3200.011.57973218
GTATCAA77300.011.5547661
TTGCGCG5950.011.17802218
ACATGGT19750.010.5858593
TGACCGA3450.010.4652219
CGCGCCA1502.4572728E-810.13114710
CTCAACG2550.010.0622274
GTACGAC856.5776374E-410.0622273
CGCGGTT1803.947207E-1010.02916918
GTCGGTT2850.09.99706712
AGGTCGC1252.5801546E-69.87786910
TCTAGAC4350.09.8309113
CGGTTTG3300.09.78934514
TGCGCGC6600.09.78923719
GTTCGCG900.00111262669.50163516
TCGCGGT1701.6636477E-89.50142517
CAAGACT5850.09.421994
ATTTGCG6900.09.3639316
TTTGCGC7250.09.30484317
Produced by FastQC (version 0.11.5)