##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364249_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4528391 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.1097347821776 28.0 20.0 31.0 2.0 31.0 2 24.795556523277252 30.0 23.0 31.0 2.0 34.0 3 24.34504816390634 29.0 22.0 31.0 2.0 34.0 4 27.412145064328588 32.0 24.0 35.0 2.0 37.0 5 27.67984721284006 33.0 26.0 35.0 2.0 37.0 6 28.41140528722012 33.0 28.0 35.0 2.0 37.0 7 28.45116333814814 35.0 28.0 35.0 2.0 37.0 8 26.55740107247806 32.0 18.0 35.0 2.0 37.0 9 26.473490915426694 32.0 17.0 37.0 2.0 39.0 10 28.107764766779194 33.0 25.0 37.0 2.0 39.0 11 28.666477784272605 34.0 27.0 37.0 2.0 39.0 12 27.278399767157914 33.0 17.0 37.0 2.0 39.0 13 26.956689914806386 33.0 17.0 37.0 2.0 39.0 14 27.413356973812554 33.0 17.0 38.0 2.0 40.0 15 26.879041363698498 32.0 17.0 38.0 2.0 40.0 16 27.70876233964779 33.0 18.0 38.0 2.0 40.0 17 28.284385116037903 34.0 23.0 39.0 2.0 40.0 18 28.859268556977522 34.0 26.0 39.0 2.0 40.0 19 29.53491295252552 36.0 27.0 40.0 2.0 41.0 20 29.564307940723317 36.0 27.0 40.0 2.0 41.0 21 29.706421331550214 36.0 27.0 40.0 2.0 41.0 22 29.625134843700554 36.0 27.0 40.0 2.0 41.0 23 29.274722302027364 36.0 27.0 40.0 2.0 41.0 24 29.207567544410367 36.0 27.0 40.0 2.0 41.0 25 28.898352858664367 36.0 26.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.1362637253959278 1101 2 -1.2399640780917167 1101 3 -3.5450949417936464 1101 4 -3.7838375697566917 1101 5 -4.569580723547304 1101 6 -2.450972272726233 1101 7 -1.9033888042287899 1101 8 -5.839328213209797 1101 9 -6.86934387021973 1101 10 -7.19421416109039 1101 11 -4.252619068510494 1101 12 -1.4075367189553987 1101 13 -0.8076720297493836 1101 14 -7.776597769810586 1101 15 -9.58605256323419 1101 16 -4.814432443093274 1101 17 -2.9475844941180007 1101 18 -7.585535357574301 1101 19 -4.278498382330831 1101 20 -3.081700410876824 1101 21 -3.0318184119180174 1101 22 -2.9502917524460095 1101 23 -7.721289521716741 1101 24 -4.608215431175395 1101 25 -3.672101973856165 1102 1 1.5750745036084908 1102 2 1.0728644929943059 1102 3 1.5604417545814648 1102 4 1.5915498631216138 1102 5 1.0958239461179353 1102 6 0.6223530682101099 1102 7 0.3638334422894012 1102 8 1.8225651133704552 1102 9 -3.556701012441625 1102 10 -0.9874861554264882 1102 11 -0.22762857388897828 1102 12 -4.219515216822529 1102 13 -6.453365667642746 1102 14 -6.463606401163844 1102 15 -1.4182182996771786 1102 16 0.1524691902157862 1102 17 -4.504867093657278 1102 18 -1.7890610613691038 1102 19 -0.9662203138657119 1102 20 -0.5552453809516393 1102 21 -0.4407277658531896 1102 22 -0.47115736010171005 1102 23 -0.20783198354693866 1102 24 -0.06755140496597178 1102 25 0.1517131829784475 1103 1 -1.2900793674930888 1103 2 0.5427892893828137 1103 3 1.5020012457088932 1103 4 2.2393759777853184 1103 5 1.853469074309885 1103 6 1.038911724572813 1103 7 1.19078684703927 1103 8 2.588220269904376 1103 9 3.641593739663797 1103 10 1.9829912710080961 1103 11 1.5579737465146835 1103 12 2.8419028214477215 1103 13 2.980388193999925 1103 14 -3.0399994865522153 1103 15 1.060770698597782 1103 16 1.693771479008987 1103 17 1.8149250041069962 1103 18 1.641713927879021 1103 19 1.183578900201983 1103 20 1.2130267234865393 1103 21 1.0924541055218384 1103 22 1.206281019251822 1103 23 1.4542622615482017 1103 24 1.0891019384695042 1103 25 1.1132164146502532 1104 1 1.3720237700886777 1104 2 0.8673533772222086 1104 3 1.4621781833932666 1104 4 1.7380449125065915 1104 5 0.9692012984411846 1104 6 0.3295562940925727 1104 7 0.07779793751931052 1104 8 1.4865554205176608 1104 9 -3.883874577070756 1104 10 -1.3251192231183708 1104 11 -0.5809405483533432 1104 12 1.0333472225338092 1104 13 1.1102127827988433 1104 14 0.930163103039046 1104 15 -4.1302356485239855 1104 16 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2.2664381626009096 1106 4 2.6001260758526428 1106 5 1.700723450969761 1106 6 1.1496389211799922 1106 7 1.111275949161353 1106 8 2.668909693279417 1106 9 3.650127587094925 1106 10 1.8585069764477709 1106 11 0.9860302544498758 1106 12 2.096984737244288 1106 13 -3.911363746980612 1106 14 0.4181946068122393 1106 15 2.4347820234788777 1106 16 1.953576793696314 1106 17 1.6368579178783094 1106 18 1.3819322507701948 1106 19 1.1230291026672496 1106 20 0.7924550397897292 1106 21 0.2733404705486464 1106 22 0.4409636230082512 1106 23 0.7369886934647241 1106 24 0.6299775823413292 1106 25 0.6565938521971546 1107 1 1.9293367410962787 1107 2 1.2535505424566935 1107 3 2.0716276660115653 1107 4 2.0044489247250468 1107 5 1.6407887724135541 1107 6 0.5997587546944061 1107 7 0.12487095228503264 1107 8 2.101713145306533 1107 9 3.2839514421024916 1107 10 1.756370376399655 1107 11 0.6591669708568979 1107 12 2.255436884412447 1107 13 2.5647892855310808 1107 14 -3.4936446465883755 1107 15 1.0424319059887033 1107 16 1.1939160607183084 1107 17 0.9055771283646479 1107 18 0.9729895030469287 1107 19 0.6438335098070702 1107 20 0.7433579006489488 1107 21 0.44819922376294485 1107 22 0.144876924309024 1107 23 0.7443849008569288 1107 24 0.8826538372282897 1107 25 0.9097261635883704 1108 1 1.60126719256996 1108 2 1.089942971513647 1108 3 1.3974315466714025 1108 4 1.8977037934419378 1108 5 1.1191237026509135 1108 6 0.35488543073503465 1108 7 0.056373188029404275 1108 8 -4.446274297984971 1108 9 -5.605231897084646 1108 10 -2.212563024966947 1108 11 -0.5462042806899134 1108 12 0.5064668018867771 1108 13 1.9177252751694915 1108 14 2.2560836947044827 1108 15 -3.6149283709124482 1108 16 -0.2225219158285192 1108 17 0.45153886750473404 1108 18 0.40582720957073093 1108 19 0.26509931714922885 1108 20 0.09844321524159838 1108 21 -0.15291175730733286 1108 22 -0.06276815874218755 1108 23 0.2091191512654973 1108 24 0.21740627557259984 1108 25 0.5775739989281767 1109 1 0.7433225011652951 1109 2 0.5947860385672534 1109 3 0.9100780028783255 1109 4 -1.154398649460994 1109 5 -2.750953089577763 1109 6 -1.034940652960227 1109 7 -0.7854734426475183 1109 8 1.4831161542255842 1109 9 -3.892982557682746 1109 10 -0.954594044396444 1109 11 -0.38875338558265327 1109 12 -6.676507400650706 1109 13 -1.6431755601793796 1109 14 0.12059630980997582 1109 15 1.6986163916372448 1109 16 1.297549596968988 1109 17 0.8651302182340359 1109 18 0.6602604260466407 1109 19 0.055924224414400925 1109 20 -0.0510718903180738 1109 21 -0.16633219063501414 1109 22 -0.19692275276527837 1109 23 -0.037691899187681344 1109 24 -0.12215467709690131 1109 25 0.10163931630084022 1110 1 0.18653188632682216 1110 2 0.08381228855248679 1110 3 -3.292962923722328 1110 4 -3.039297558276658 1110 5 -0.7789661052446633 1110 6 -0.8137527159220355 1110 7 -0.9769541775838029 1110 8 -4.93977736988057 1110 9 -8.08024820669526 1110 10 -3.431699807054212 1110 11 -1.464058132413932 1110 12 0.9035900418853124 1110 13 1.3390152971147842 1110 14 1.4543924386319986 1110 15 2.13133596860801 1110 16 1.6800701699293619 1110 17 0.9707452974266424 1110 18 0.5489976036778614 1110 19 -0.22598598562693795 1110 20 -0.23660482143083428 1110 21 -0.41243875746646097 1110 22 -0.7261959117079329 1110 23 -0.17981722680264411 1110 24 -0.11240200953824342 1110 25 0.16148907312076943 1111 1 1.327197431007182 1111 2 1.1744372844215967 1111 3 1.3005677948938192 1111 4 2.1486071311972204 1111 5 -1.5077559154528721 1111 6 -0.30721125963697915 1111 7 -0.6561657681326807 1111 8 1.8023970179877047 1111 9 3.269484133697084 1111 10 1.8964001666402837 1111 11 1.649401208838075 1111 12 2.842679195072794 1111 13 3.074958430959569 1111 14 3.2478830490651056 1111 15 3.5469267496964214 1111 16 2.652889963565542 1111 17 2.0388496201215958 1111 18 1.4173088123342694 1111 19 1.0286775682951586 1111 20 1.0726421581729255 1111 21 0.7635385669244705 1111 22 0.6685919852925792 1111 23 1.0030607760235455 1111 24 0.9136166457790154 1111 25 1.0299976832163544 1112 1 2.1455091790998253 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3.5884015891360868 2204 9 -2.337744451842614 2204 10 0.3772824038068272 2204 11 1.681020511255273 2204 12 3.4048667366521883 2204 13 3.6861555942662285 2204 14 3.8447592529239074 2204 15 4.299379744483819 2204 16 3.901760014086218 2204 17 2.9611289078682965 2204 18 2.8747341190669253 2204 19 2.0572038423249595 2204 20 2.106867071516355 2204 21 1.6714561630336995 2204 22 1.5393017827011661 2204 23 2.0888883369034836 2204 24 2.073915035048831 2204 25 2.112614019266992 2205 1 -3.1504228427789656 2205 2 -3.753106575056904 2205 3 -0.33213405466417 2205 4 -2.025701128323341 2205 5 -2.88787757321165 2205 6 -1.0171046081072923 2205 7 -0.7547977937185877 2205 8 -4.686757666766404 2205 9 0.74098394183736 2205 10 0.3381339734040978 2205 11 0.2853139397329585 2205 12 1.9992586112205863 2205 13 -4.324543679281266 2205 14 -0.31303414198751867 2205 15 2.1963151542804376 2205 16 1.828237134572042 2205 17 1.7673065179243892 2205 18 0.3963670344932382 2205 19 0.6358284951705073 2205 20 0.5276859022978613 2205 21 0.525145728981478 2205 22 0.5411141664053467 2205 23 0.7913917660856313 2205 24 0.4795461248228641 2205 25 0.9031953715633314 2206 1 -6.8389982041003705 2206 2 -5.491958818424408 2206 3 -2.2570817929094638 2206 4 -0.7299547582920738 2206 5 -0.4722383129270611 2206 6 -1.0656792133194628 2206 7 -1.5543631168307002 2206 8 -2.887935220976427 2206 9 -5.642640106904189 2206 10 -2.5285205783305287 2206 11 -1.7050460112230468 2206 12 -7.581300016028731 2206 13 -6.576026383712151 2206 14 -7.440616577226333 2206 15 -2.3083379247596127 2206 16 -6.179141976020102 2206 17 -2.7647284555645335 2206 18 -1.95309823302766 2206 19 -1.958818337376215 2206 20 -1.88806978162442 2206 21 -1.8357627362628435 2206 22 -1.9027792634350185 2206 23 -1.4791930070772992 2206 24 -1.3638853050913937 2206 25 -1.6623034500232414 2207 1 -6.685608925639244 2207 2 -5.079343164839305 2207 3 -0.9814436701410365 2207 4 0.07712591265335789 2207 5 0.5355486110797294 2207 6 0.17300465681125843 2207 7 0.14245826361698022 2207 8 1.680485302914633 2207 9 -1.7269859046834064 2207 10 -0.5349187049394253 2207 11 -0.36564647342836665 2207 12 -4.204454039931775 2207 13 -4.800631935543485 2207 14 -1.1416173954069642 2207 15 -4.770989866184138 2207 16 -0.9037216376943036 2207 17 -0.05801521244777774 2207 18 0.1406906694808292 2207 19 -0.0833405211793945 2207 20 -0.09680221302160419 2207 21 -0.2812315640974603 2207 22 -0.27755470148312966 2207 23 -0.34466376088702333 2207 24 -0.4606924488229538 2207 25 0.015337902126091763 2208 1 -4.025817251719417 2208 2 -4.551712126788953 2208 3 -1.532296346146488 2208 4 -0.31620794181267087 2208 5 -3.2365530623474292 2208 6 -1.2884630192763034 2208 7 -0.8315880142803138 2208 8 -5.151868589979458 2208 9 -8.209717831177421 2208 10 -4.02297322680565 2208 11 -1.7145083261864862 2208 12 -0.12191382020208508 2208 13 0.8599780393304748 2208 14 0.8193451848567754 2208 15 1.295501704275523 2208 16 -4.634662717949833 2208 17 -6.913829365141847 2208 18 -2.959163080539696 2208 19 -2.5115962221630888 2208 20 -1.5593606680164314 2208 21 -1.4678008315009379 2208 22 -1.0894048386089068 2208 23 -0.8543315125169286 2208 24 -0.7588518825838122 2208 25 -0.5789152566934774 2209 1 0.8874179742289421 2209 2 0.8826331794272946 2209 3 -2.2793099539336232 2209 4 0.28443624984537763 2209 5 0.7019357740144159 2209 6 0.41129217843877086 2209 7 0.45258657247958567 2209 8 1.9799011314630661 2209 9 -3.5204143988634407 2209 10 -0.5620896562798876 2209 11 0.19211214294567114 2209 12 -4.270548202434814 2209 13 0.2995461619811195 2209 14 1.598498567105917 2209 15 2.559899425915024 2209 16 -2.7953387672595937 2209 17 -0.06312162232737961 2209 18 0.5325999699683379 2209 19 0.427547206211365 2209 20 0.6038947611459271 2209 21 0.5889643693329276 2209 22 0.451362857259511 2209 23 0.9200333994181982 2209 24 0.4314439562894812 2209 25 0.5210822351402093 2210 1 -0.22871102345204264 2210 2 -2.981054456688195 2210 3 -0.8223317005803494 2210 4 -3.1063414864747365 2210 5 -3.561274603322019 2210 6 -1.4991513406684867 2210 7 -0.7494738644202137 2210 8 -5.048221306254465 2210 9 -4.561184273576863 2210 10 -1.7555350808917858 2210 11 -0.6940396033775365 2210 12 0.8900454364814543 2210 13 -6.950017826820808 2210 14 -1.5236304228480968 2210 15 -5.305413215424952 2210 16 -1.4492237245646358 2210 17 -0.7587144486759954 2210 18 -0.2107355150790049 2210 19 -0.5126881533949934 2210 20 -0.5080381275432053 2210 21 -0.6303134740265328 2210 22 -0.376866519642693 2210 23 -0.06071839288438241 2210 24 -0.07913295841956014 2210 25 0.006905111730130642 2211 1 0.28737873870935005 2211 2 0.10716591757501348 2211 3 -3.6165230809327547 2211 4 -2.9819394040127634 2211 5 -0.7674096102359371 2211 6 -0.8545860743109017 2211 7 -1.0741444392948658 2211 8 -4.951084809341808 2211 9 0.16234407142880158 2211 10 -0.021627174939904847 2211 11 -5.99028619594009 2211 12 -0.557719006776491 2211 13 0.6074273570566575 2211 14 1.1853802104016253 2211 15 -4.2991150712531585 2211 16 -1.0307279575540491 2211 17 -5.915909866984954 2211 18 -1.9819109043772443 2211 19 -1.2544287643605898 2211 20 -0.702599237504888 2211 21 -0.6483325265148423 2211 22 -0.39357373209658775 2211 23 -0.19441659303170766 2211 24 -0.19085741837893622 2211 25 0.028792277420212287 2212 1 2.079744671652765 2212 2 1.4052701887948267 2212 3 2.2868687037523188 2212 4 2.662391765075455 2212 5 -1.1777965824814238 2212 6 0.4161614206706581 2212 7 1.1300666720201953 2212 8 -1.3358481558947375 2212 9 2.9135668083647666 2212 10 2.3902630074046947 2212 11 2.103896614842199 2212 12 3.589975020610254 2212 13 3.5816243789215143 2212 14 3.9492737772219115 2212 15 4.481367877769834 2212 16 3.6527366539533013 2212 17 2.784693462689823 2212 18 2.5410639555409773 2212 19 2.093236159719808 2212 20 1.8264175440011563 2212 21 1.5938578467041005 2212 22 1.6383215344806317 2212 23 1.763148088292617 2212 24 1.8909971787603155 2212 25 2.194505972980611 2213 1 2.471686867474272 2213 2 2.0330191615546056 2213 3 2.6221856871576286 2213 4 2.8654141440768974 2213 5 1.955639225119704 2213 6 -0.36417783084510447 2213 7 0.9216109340106584 2213 8 -5.7954337082967236 2213 9 0.905630459795411 2213 10 1.0649084559287942 2213 11 1.110485188274513 2213 12 2.849147354206327 2213 13 -2.0227988230709286 2213 14 1.1434487781959533 2213 15 -3.4375389618068617 2213 16 0.13500196215533933 2213 17 1.2289527558046913 2213 18 1.4464002849291901 2213 19 1.2734237603084289 2213 20 1.5227068166461457 2213 21 1.2434294927760092 2213 22 1.3346090088932812 2213 23 1.629231852951957 2213 24 1.7067780911234607 2213 25 1.8989999054449989 2214 1 1.9553943192641157 2214 2 1.155084846953816 2214 3 0.7607557232794129 2214 4 1.7447415908976076 2214 5 1.5476342095706848 2214 6 0.23144120858306394 2214 7 0.32924865683672877 2214 8 2.195333190171855 2214 9 3.0584178163526197 2214 10 1.6200245480052082 2214 11 1.6475117895233495 2214 12 2.7313810618628906 2214 13 1.023568248498254 2214 14 2.327570552012986 2214 15 3.515303751449533 2214 16 2.3352085771102473 2214 17 1.3347174973414653 2214 18 1.099812264364619 2214 19 1.0618517529147944 2214 20 0.41215157052384654 2214 21 0.31804233716839647 2214 22 0.19096970522731738 2214 23 -6.0681127319205785 2214 24 -2.449314899940287 2214 25 -0.9170592709346863 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 506908.0 3 10.0 4 4030.0 5 22260.0 6 28751.0 7 27321.0 8 23289.0 9 19434.0 10 17675.0 11 16415.0 12 15771.0 13 14473.0 14 13679.0 15 13532.0 16 13621.0 17 14878.0 18 18250.0 19 24961.0 20 36487.0 21 51067.0 22 66800.0 23 82388.0 24 98018.0 25 114259.0 26 128099.0 27 141532.0 28 153242.0 29 173860.0 30 185802.0 31 202050.0 32 245699.0 33 293786.0 34 338006.0 35 371344.0 36 389587.0 37 359909.0 38 301198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32526300153917 18.195104274191458 15.364252953433736 23.115379770835634 2 15.088684953003039 20.60493862145796 40.53744524991543 23.76893117562357 3 17.058208677312216 25.938681259851098 30.624078267220973 26.379031795615717 4 10.255399059368605 16.25608942290394 39.23495933590396 34.2535521818235 5 11.746738979045633 38.40441863384872 35.4366232985773 14.41221908852834 6 31.36978081938735 36.18735992093666 18.779293537497608 13.663565722178381 7 28.869834242591836 32.62125372465226 20.99088098366923 17.51803104908667 8 25.522979097669058 33.23039386252476 23.063040994243348 18.18358604556284 9 26.26945679356915 15.457936555402716 23.132601910736316 35.140004740291815 10 14.910535370218975 30.166357276876653 32.67317872925074 22.249928623653624 11 34.42974903114447 23.687496165442894 23.071464183963055 18.811290619449583 12 22.887293839242563 25.992812087883365 30.899250952754958 20.22064312011911 13 27.530182606207248 21.579400609366598 27.8356854222464 23.054731362179755 14 21.61679393336831 21.212413835566714 29.300060087808376 27.8707321432566 15 23.05178855792465 28.389290135966284 26.056136201050258 22.502785105058816 16 23.10085873982389 28.63684244564991 26.29234009038341 21.96995872414279 17 22.11531050587932 28.659193452794902 26.95798675923801 22.267509282087765 18 21.702683433911258 27.887943340008015 28.384480162336246 22.024893063744486 19 23.376813776730508 27.48644650355226 27.521836188721366 21.61490353099587 20 23.81683140785081 26.98369087757556 27.355862450066372 21.843615264507257 21 24.59146955791251 26.57193663905073 26.74995963586568 22.08663416717108 22 23.87215247403528 26.892702767591913 26.66475008039917 22.570394677973642 23 23.213932510305565 26.88936126196705 27.08082075966717 22.81588546806022 24 23.14028480507281 26.953596632539174 27.202337589099873 22.703780973288147 25 23.029302837479655 26.79732626752342 27.52841876219715 22.64495213279978 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11657.0 1 11657.0 2 7620.5 3 3584.0 4 3584.0 5 3584.0 6 4673.5 7 5763.0 8 5763.0 9 5763.0 10 7415.0 11 9067.0 12 9067.0 13 9067.0 14 14210.5 15 19354.0 16 19354.0 17 19354.0 18 34513.5 19 49673.0 20 49673.0 21 49673.0 22 79943.5 23 110214.0 24 110214.0 25 110214.0 26 160234.5 27 210255.0 28 210255.0 29 210255.0 30 263582.0 31 316909.0 32 316909.0 33 316909.0 34 370995.5 35 425082.0 36 425082.0 37 425082.0 38 470363.0 39 515644.0 40 515644.0 41 515644.0 42 547388.0 43 579132.0 44 579132.0 45 579132.0 46 590165.0 47 601198.0 48 601198.0 49 601198.0 50 571984.5 51 542771.0 52 542771.0 53 542771.0 54 487553.5 55 432336.0 56 432336.0 57 432336.0 58 369858.0 59 307380.0 60 307380.0 61 307380.0 62 249053.0 63 190726.0 64 190726.0 65 190726.0 66 146846.5 67 102967.0 68 102967.0 69 102967.0 70 77021.5 71 51076.0 72 51076.0 73 51076.0 74 37293.5 75 23511.0 76 23511.0 77 23511.0 78 17775.0 79 12039.0 80 12039.0 81 12039.0 82 8585.0 83 5131.0 84 5131.0 85 5131.0 86 3627.5 87 2124.0 88 2124.0 89 2124.0 90 1400.5 91 677.0 92 677.0 93 677.0 94 389.0 95 101.0 96 101.0 97 101.0 98 60.5 99 20.0 100 20.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24339771013589592 2 0.25401958443959455 3 0.25973905521850915 4 0.32742313991879235 5 0.27038301242096807 6 0.29878162022669863 7 0.2990024492143015 8 0.2967941593382727 9 0.30697437566676555 10 0.30010659415231594 11 0.29873745442917804 12 0.3000845112535556 13 0.2989803663155412 14 0.2944092062721616 15 0.29372463641059265 16 0.295977092084142 17 0.29244382828249593 18 0.29410004568951753 19 0.2859956218444918 20 0.33455591621836545 21 0.2924659111812562 22 0.29633041846430663 23 0.30028325734239825 24 0.3015198996729744 25 0.2982074648589311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4528391.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.91277834652051 #Duplication Level Percentage of deduplicated Percentage of total 1 95.86576402460811 82.36094135665996 2 2.4860460850358144 4.27166252525834 3 0.6770379732693019 1.7449863998898905 4 0.3300754397057645 1.1343079235628657 5 0.16190637291408919 0.6954913164528618 6 0.09799478137109226 0.5051402358630233 7 0.07174519900871616 0.4314680565903983 8 0.04431820297964594 0.3045999959445141 9 0.03714353487517157 0.2871993850863183 >10 0.19154461842495388 3.0195150048046284 >50 0.018899795431642323 1.1291960824959713 >100 0.01581136125418834 2.6587464633585265 >500 0.0013096445184988218 0.7284274154109878 >1k 3.777811903247328E-4 0.5142365345433944 >5k 2.5185412688017925E-5 0.21408130407863177 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 9894 0.21848820033429092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.2082898760288147E-5 0.0 2 0.0 0.0 0.0 2.2082898760288147E-5 0.0 3 0.0 0.0 0.0 2.2082898760288147E-5 0.0 4 0.0 0.0 0.0 2.2082898760288147E-5 0.0 5 0.0 0.0 0.0 2.2082898760288147E-5 0.0 6 0.0 0.0 0.0 2.2082898760288147E-5 0.0 7 0.0 0.0 0.0 2.2082898760288147E-5 0.0 8 0.0 0.0 0.0 2.2082898760288147E-5 0.0 9 0.0 0.0 0.0 1.1041449380144073E-4 0.0 10 0.0 0.0 0.0 1.3249739256172887E-4 0.0 11 0.0 0.0 0.0 1.3249739256172887E-4 0.0 12 0.0 0.0 0.0 1.3249739256172887E-4 0.0 13 0.0 0.0 0.0 1.5458029132201702E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2765 0.0 12.646358 1 TTGACCG 320 0.0 11.579732 18 GTATCAA 7730 0.0 11.554766 1 TTGCGCG 595 0.0 11.178022 18 ACATGGT 1975 0.0 10.585859 3 TGACCGA 345 0.0 10.46522 19 CGCGCCA 150 2.4572728E-8 10.131147 10 CTCAACG 255 0.0 10.062227 4 GTACGAC 85 6.5776374E-4 10.062227 3 CGCGGTT 180 3.947207E-10 10.029169 18 GTCGGTT 285 0.0 9.997067 12 AGGTCGC 125 2.5801546E-6 9.877869 10 TCTAGAC 435 0.0 9.830911 3 CGGTTTG 330 0.0 9.789345 14 TGCGCGC 660 0.0 9.789237 19 GTTCGCG 90 0.0011126266 9.501635 16 TCGCGGT 170 1.6636477E-8 9.501425 17 CAAGACT 585 0.0 9.42199 4 ATTTGCG 690 0.0 9.36393 16 TTTGCGC 725 0.0 9.304843 17 >>END_MODULE