FastQCFastQC Report
Thu 26 May 2016
SRR1364249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4528391
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT125070.27619081479492386No Hit
TATCAACGCAGAGTACTTTTTTTTT97730.21581616958429606No Hit
GGTATCAACGCAGAGTACTTTTTTT88520.19547781982607068No Hit
GTGGTATCAACGCAGAGTACATGGG71760.15846688150382773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51950.012.4837991
TGGTATC17900.012.079242
ATCCCGA1209.405994E-911.92681316
GTGGTAT19000.011.8768031
CGACTAA658.1823004E-411.66590810
TCCCGAT1407.8180165E-910.90451417
CGCGCCA2000.010.90033210
TATACCG700.001515168210.8374165
CGGTGTT2900.010.52802914
CGAAATC2400.010.26797513
GTCGGTT3350.010.18556712
ACGGCCC3100.010.15702815
GGTCGGT3750.010.11011811
ACGAAAT2650.010.0147412
CGTATAC951.6762436E-49.9816093
TAGACAG7300.09.7425395
GCTTATA7150.09.6803931
TCCGGTG3450.09.615602512
GTATCAA103800.09.57282451
CGCCCGA800.0043588489.54145116
Produced by FastQC (version 0.11.5)