FastQCFastQC Report
Thu 26 May 2016
SRR1364248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607675
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN56550.21685984641490985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG5750.011.73188418
CGAGCGC658.040324E-411.68992710
ACATGGT14500.011.6655313
TGCGCGC6050.011.30718219
GACACCG909.535749E-510.5538137
GGTATCA22150.010.5527031
GTCTTGG4700.010.5125441
TACATGT17350.010.350782
CATGGTA10750.010.3426054
ATTTGCG6250.010.33747716
CTCAACG2053.6379788E-1210.1981484
TCGACGG750.00264503710.13497514
CGACGGT750.002645881610.13458515
AAGTCGT750.002652648110.1314666
TTTGCGC6700.09.92621817
GACCGAA1151.0254826E-59.914268519
GTATCAA48400.09.8158291
TCGTGAC2151.0913936E-119.71876513
ATTAACC2450.09.6967553
CCGTCTA800.0045233279.4984329
Produced by FastQC (version 0.11.5)