##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364248_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2607675 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.580612231202124 29.0 23.0 31.0 7.0 33.0 2 25.291028214789037 30.0 25.0 31.0 7.0 34.0 3 24.78321569980922 30.0 23.0 31.0 7.0 34.0 4 27.88401276999626 33.0 25.0 35.0 7.0 37.0 5 28.366083963684126 33.0 27.0 35.0 2.0 37.0 6 29.024650311100885 35.0 28.0 35.0 2.0 37.0 7 29.034079016748635 35.0 29.0 35.0 2.0 37.0 8 27.29842369160267 33.0 23.0 35.0 2.0 37.0 9 27.124948469421994 32.0 17.0 37.0 2.0 39.0 10 28.82998456479431 34.0 27.0 37.0 2.0 39.0 11 29.366043314446777 35.0 27.0 38.0 2.0 39.0 12 27.913258362334265 34.0 22.0 37.0 2.0 39.0 13 27.69650320687969 33.0 19.0 37.0 2.0 39.0 14 28.149927042288628 33.0 19.0 38.0 2.0 40.0 15 27.671151888176247 33.0 17.0 38.0 2.0 40.0 16 28.454952783609922 34.0 23.0 38.0 2.0 40.0 17 29.01488260615299 34.0 26.0 39.0 2.0 40.0 18 29.57643571380636 35.0 27.0 39.0 2.0 41.0 19 30.255114230109196 36.0 28.0 40.0 2.0 41.0 20 30.283791116607706 36.0 28.0 40.0 2.0 41.0 21 30.452264565177792 36.0 29.0 40.0 2.0 41.0 22 30.335464350427106 36.0 29.0 40.0 2.0 41.0 23 29.95211519840471 36.0 28.0 40.0 2.0 41.0 24 29.900719606546062 36.0 28.0 40.0 2.0 41.0 25 29.58471780417422 36.0 27.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6749700574218984 1101 2 -0.8341356260957191 1101 3 -3.240606616693565 1101 4 -3.511616819139153 1101 5 -4.071949737586067 1101 6 -1.9382657588527863 1101 7 -1.5598259256316958 1101 8 -5.108613684622998 1101 9 -6.326865736756826 1101 10 -6.603713485636785 1101 11 -3.5357326042357364 1101 12 -0.7266205423235021 1101 13 -0.18411087412358285 1101 14 -7.540120130057016 1101 15 -9.600536883388266 1101 16 -4.52276773671354 1101 17 -2.541471476493335 1101 18 -7.230301471491103 1101 19 -3.617832228078786 1101 20 -2.616612361414404 1101 21 -2.4450633053167756 1101 22 -2.3799293630329075 1101 23 -7.406794010154112 1101 24 -4.053448361779729 1101 25 -2.9491784291710097 1102 1 1.992370926870798 1102 2 1.4402923895047017 1102 3 1.97401652292481 1102 4 2.1632245093937534 1102 5 1.4928270021750727 1102 6 1.0113714569002887 1102 7 0.8497938470124886 1102 8 2.223078569763615 1102 9 -2.766364476468535 1102 10 -0.24717539309024872 1102 11 0.47733091057076393 1102 12 -4.073544784120394 1102 13 -6.033613707806293 1102 14 -5.970579153916955 1102 15 -0.8347216936806134 1102 16 0.851871091913253 1102 17 -4.005471934517374 1102 18 -1.1928600525088768 1102 19 -0.42688779984332115 1102 20 0.01934936228379769 1102 21 0.15536315935711542 1102 22 0.22526859996375848 1102 23 0.5018749293003744 1102 24 0.41490765770570803 1102 25 0.7583608647658338 1103 1 -0.7658411239251848 1103 2 0.9745612581846856 1103 3 1.923084773107533 1103 4 2.8271433913230943 1103 5 2.3988872707147024 1103 6 1.6129797972950897 1103 7 1.5815781708441428 1103 8 2.9278884488305117 1103 9 4.107261085172986 1103 10 2.5856155903611615 1103 11 2.221792753890618 1103 12 3.4983416907655283 1103 13 3.700693088966105 1103 14 -2.668538680784021 1103 15 1.7384747927657358 1103 16 2.39510438535293 1103 17 2.422726401014476 1103 18 2.120960696851224 1103 19 1.8603079382329888 1103 20 1.635790789952523 1103 21 1.6123671894899338 1103 22 1.7880348609963903 1103 23 2.036386217457782 1103 24 1.7595309684132268 1103 25 1.8766703055849057 1104 1 1.5543956983549982 1104 2 0.9809865260771922 1104 3 1.6528130568045363 1104 4 2.0434227361828405 1104 5 1.0993934053460954 1104 6 0.5699326072578792 1104 7 0.36182091212218737 1104 8 1.655326146823679 1104 9 -3.735289755904464 1104 10 -1.0599372075634044 1104 11 -0.3893958425072128 1104 12 1.280508537164419 1104 13 1.458324897347147 1104 14 1.1306135066787562 1104 15 -3.9077079398196766 1104 16 -4.715794354117165 1104 17 -1.469092773395694 1104 18 -0.7643640739989159 1104 19 -0.46240833116207725 1104 20 -0.17151710859439362 1104 21 -0.6414090847819729 1104 22 -0.6194895368165554 1104 23 -0.7134870919443514 1104 24 -7.220980651117891 1104 25 -4.20367149459053 1105 1 -2.3675070429667286 1105 2 -0.04532352577501797 1105 3 0.9766021740822488 1105 4 1.455461269843358 1105 5 1.3649611524310643 1105 6 1.1084295054876598 1105 7 0.17923348996325217 1105 8 2.5709123471348043 1105 9 2.931073879842419 1105 10 1.7890992897971714 1105 11 1.676242817205118 1105 12 2.219704436138432 1105 13 2.2824616662822272 1105 14 2.7317135258782557 1105 15 3.303649596864588 1105 16 2.8801296877906566 1105 17 1.6451428305438007 1105 18 1.8362869534455086 1105 19 0.8236722629639601 1105 20 0.8409992839084417 1105 21 0.8290809331760016 1105 22 0.6457898362079462 1105 23 1.2149164682392843 1105 24 1.3065901733139071 1105 25 1.3529399249035414 1106 1 2.305777063412794 1106 2 1.5717899604681413 1106 3 2.283534028552733 1106 4 2.619130503370723 1106 5 1.5473173932920474 1106 6 1.1082925953567297 1106 7 1.1585747666519737 1106 8 2.6148275847071467 1106 9 3.6941304142723403 1106 10 1.7381309044823432 1106 11 0.9023871898135702 1106 12 2.1233473661132756 1106 13 -4.003457809551559 1106 14 0.5147071166633275 1106 15 2.598879661909155 1106 16 2.066819782080067 1106 17 1.7650204307858282 1106 18 1.4176553404410797 1106 19 1.1042487421536222 1106 20 0.6784627978837783 1106 21 0.3188438370433815 1106 22 0.3772772054605227 1106 23 0.8698990866052725 1106 24 0.808419726853824 1106 25 0.8966163575927766 1107 1 2.181251847737549 1107 2 1.6274840200890992 1107 3 2.3715649817214377 1107 4 2.348299568858163 1107 5 1.8721589618074717 1107 6 1.0029540591936872 1107 7 0.6198457748563051 1107 8 2.376488222135162 1107 9 3.760340053960956 1107 10 2.0179920328001835 1107 11 0.8905057394148663 1107 12 2.544532257259039 1107 13 2.8790609449758335 1107 14 -3.3637378936194153 1107 15 1.3203337554026788 1107 16 1.5645976238263977 1107 17 1.3102473725917712 1107 18 1.448064857407239 1107 19 1.172053810212116 1107 20 1.2008567311385043 1107 21 0.8956858398971868 1107 22 0.5513078875321149 1107 23 1.1658691770147023 1107 24 1.3731887987543168 1107 25 1.3213861801116202 1108 1 1.5354436762535713 1108 2 0.9462300641808419 1108 3 1.361896342234111 1108 4 1.8078393150969347 1108 5 0.9974743294001058 1108 6 0.29645531897118715 1108 7 0.032558053713394486 1108 8 -4.3049074175957465 1108 9 -5.540728852435553 1108 10 -2.1853852900762263 1108 11 -0.6676479224042353 1108 12 0.3955384804376294 1108 13 1.9572082491272234 1108 14 2.2146638112888724 1108 15 -3.820067953267543 1108 16 -0.27601414180890416 1108 17 0.3442467876111017 1108 18 0.3825376177165829 1108 19 0.36822284517270276 1108 20 0.18704827186660822 1108 21 -0.06617906342582103 1108 22 -0.05104877574567723 1108 23 0.1456911529138729 1108 24 0.14524374555587372 1108 25 0.45890178037984697 1109 1 0.8287664111617481 1109 2 0.6571491314539486 1109 3 0.8695798496861009 1109 4 -1.313317428642172 1109 5 -2.421887908261393 1109 6 -0.8928118345401721 1109 7 -0.795632821772184 1109 8 1.6229588869658578 1109 9 -3.4880934062224433 1109 10 -0.7586788021422244 1109 11 -0.24876640236743697 1109 12 -7.017616241836624 1109 13 -1.7315660963229753 1109 14 0.28410809787945723 1109 15 1.8530148297404594 1109 16 1.4876923790990162 1109 17 1.0514786225488137 1109 18 0.6575578639358675 1109 19 0.19893175867743906 1109 20 0.08446552545731478 1109 21 0.012913765635751417 1109 22 -0.0038457006121497272 1109 23 0.15109181290671359 1109 24 -0.01914727646485659 1109 25 0.22130669681434867 1110 1 0.028387963974790154 1110 2 -0.032284953724815324 1110 3 -3.551254986042352 1110 4 -3.264246858295003 1110 5 -0.8743476139125868 1110 6 -0.9065159120740383 1110 7 -1.0677000683075129 1110 8 -4.87035005953458 1110 9 -8.271110108388083 1110 10 -3.7542789849764517 1110 11 -1.73697913526102 1110 12 0.5909255390455819 1110 13 0.9267027959884722 1110 14 1.2362270670197013 1110 15 1.9196504878654785 1110 16 1.488041292213886 1110 17 0.7730308994202417 1110 18 0.31437100798305195 1110 19 -0.5186864546854544 1110 20 -0.5489014972714088 1110 21 -0.6664763222649732 1110 22 -0.9632334472339394 1110 23 -0.44715495342515865 1110 24 -0.40008551272224224 1110 25 -0.11975236552130752 1111 1 1.0698631538316157 1111 2 1.0309292881651189 1111 3 1.0949164087010743 1111 4 2.0551420901398494 1111 5 -1.7693625829914446 1111 6 -0.5690609325627847 1111 7 -0.8885609871587015 1111 8 1.4943301712975448 1111 9 3.033924753490446 1111 10 1.639434045884876 1111 11 1.3121575752888504 1111 12 2.6100874113701344 1111 13 2.8341570761950265 1111 14 2.8773732757986608 1111 15 3.336081069559441 1111 16 2.3870713258112985 1111 17 1.7617637812988818 1111 18 1.1403206219800524 1111 19 0.673811765166267 1111 20 0.6972285549301453 1111 21 0.4352051316906511 1111 22 0.5181973741588024 1111 23 0.79970756547036 1111 24 0.8574753564739126 1111 25 0.8452591334229176 1112 1 2.2473951508699095 1112 2 1.571340754211235 1112 3 2.2142904831669092 1112 4 2.5855577871992956 1112 5 1.9221727459698421 1112 6 1.0639541933098613 1112 7 0.9091159220860625 1112 8 -3.677977846472711 1112 9 1.6903096170402634 1112 10 1.0510851988360095 1112 11 1.2012005729617918 1112 12 2.219091536608129 1112 13 2.549371535683072 1112 14 2.833185630357576 1112 15 3.654357724943406 1112 16 2.653105544131588 1112 17 1.9726903732176453 1112 18 1.549032542860104 1112 19 1.2568189578889246 1112 20 1.2216600125142385 1112 21 1.0524663438759667 1112 22 0.8012944759146734 1112 23 1.0395147899256187 1112 24 1.1950467548238173 1112 25 1.2471789557203579 1113 1 -0.02286423496971679 1113 2 0.19822200293598513 1113 3 -6.445836786777999 1113 4 -3.843927011821311 1113 5 -1.2009332528059176 1113 6 -1.185396449416114 1113 7 -0.32985903200493993 1113 8 1.4576556977019486 1113 9 -6.289456502119091 1113 10 -2.2155103939001677 1113 11 -0.5669199636633877 1113 12 -4.760652903302091 1113 13 -0.2657552241618575 1113 14 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1.9851349241751386 2204 7 1.9478193901482328 2204 8 3.5306926500953892 2204 9 -2.3129511153260403 2204 10 0.5764816072799874 2204 11 1.7059297564013356 2204 12 3.4948497274573036 2204 13 3.6970388834990757 2204 14 3.9612013365923104 2204 15 4.388681254957191 2204 16 4.005342505636985 2204 17 2.9906215441576975 2204 18 2.7964813884313813 2204 19 2.2166749272133224 2204 20 2.1665703259566484 2204 21 1.797226127864068 2204 22 1.6982787696799377 2204 23 2.282985393612698 2204 24 2.1392043357088824 2204 25 2.179436436991594 2205 1 -2.8968240887428536 2205 2 -3.495303187997372 2205 3 0.07093953399725805 2205 4 -1.9222239668718295 2205 5 -2.516629207749375 2205 6 -0.6657316308343795 2205 7 -0.38194788924368694 2205 8 -4.006825874176688 2205 9 1.3344761055331595 2205 10 1.012583015185001 2205 11 0.8242590776340357 2205 12 2.4622309993528724 2205 13 -4.146025929108621 2205 14 0.12260789168066566 2205 15 2.6958932294263747 2205 16 2.3280985778292127 2205 17 2.259035018540846 2205 18 0.7747845091975307 2205 19 0.9794891459703265 2205 20 0.9745475667550068 2205 21 0.8454098300765551 2205 22 0.9551482869640147 2205 23 1.1678216805187454 2205 24 0.717355746699095 2205 25 1.1657047634609263 2206 1 -6.979563349441612 2206 2 -5.736331332676393 2206 3 -2.1867054728200763 2206 4 -0.7608410605550553 2206 5 -0.5292328514210674 2206 6 -1.122276259665746 2206 7 -1.650712539073723 2206 8 -2.9818884051349386 2206 9 -5.918809363091572 2206 10 -2.5973712304331826 2206 11 -1.8581462795153811 2206 12 -7.5919552724098445 2206 13 -6.703844204623216 2206 14 -7.855747465552643 2206 15 -2.527597813989111 2206 16 -6.568332868800024 2206 17 -3.0596621154945005 2206 18 -2.057550035385681 2206 19 -2.268982537415173 2206 20 -2.1662420812191776 2206 21 -2.037411845375381 2206 22 -2.0226934631962976 2206 23 -1.6316765209268738 2206 24 -1.5105206377012017 2206 25 -1.6442874653185093 2207 1 -6.975710385187938 2207 2 -5.303450643774628 2207 3 -1.0712738258036794 2207 4 -0.08046422172266787 2207 5 0.40988205272051204 2207 6 -0.06616228202083718 2207 7 -0.02065131224588157 2207 8 1.4299067458724402 2207 9 -1.7380832929578318 2207 10 -0.5781256019561134 2207 11 -0.43558310639717845 2207 12 -4.245693747788298 2207 13 -4.7795175969271995 2207 14 -1.055761105655165 2207 15 -5.047336505730957 2207 16 -0.9817553496857592 2207 17 -0.08671574530494297 2207 18 0.0664119183930616 2207 19 -0.1941287365655029 2207 20 -0.18909294561736445 2207 21 -0.3567784340263138 2207 22 -0.35271300838732245 2207 23 -0.49700558224574465 2207 24 -0.6603532888698496 2207 25 -0.07573220519213564 2208 1 -4.083472274145397 2208 2 -4.54630360408299 2208 3 -1.5151206064066471 2208 4 -0.22158871351484422 2208 5 -3.096734109773884 2208 6 -1.2222705567411474 2208 7 -0.9104833943799662 2208 8 -5.4069107173153945 2208 9 -8.589876824502277 2208 10 -4.082076400837526 2208 11 -1.836040551298563 2208 12 -0.18945518854999932 2208 13 0.9112453879581182 2208 14 0.8006667566583303 2208 15 1.2088402285141058 2208 16 -4.972405831097959 2208 17 -7.445622216049852 2208 18 -3.2958855860931706 2208 19 -2.5894624132598203 2208 20 -1.6361164616747033 2208 21 -1.5068850639999738 2208 22 -1.0937473522836534 2208 23 -0.8539163975871347 2208 24 -0.859044247012541 2208 25 -0.5480924174687871 2209 1 0.7269004874873133 2209 2 0.8401420678401372 2209 3 -2.449861815683665 2209 4 0.25727371494970797 2209 5 0.472111169615161 2209 6 0.26229163173350756 2209 7 0.2704357086213669 2209 8 1.7842553226570104 2209 9 -3.7193681333702457 2209 10 -0.6732579867140736 2209 11 -0.0028942546244152823 2209 12 -4.284746722833578 2209 13 0.1754285964384117 2209 14 1.4017406385677553 2209 15 2.3128860500665596 2209 16 -3.5633250798421408 2209 17 -0.39097957752127144 2209 18 0.13689173826240264 2209 19 0.18805869516314289 2209 20 0.4092010634173562 2209 21 0.30319499740634726 2209 22 0.0970251819902046 2209 23 0.6058427866415457 2209 24 0.09976760868260115 2209 25 0.1851920920093555 2210 1 -0.7803746447878197 2210 2 -3.422820544260407 2210 3 -1.3251442599316263 2210 4 -3.692075786123933 2210 5 -3.9479079874729592 2210 6 -2.0434165489746725 2210 7 -1.3123029129103578 2210 8 -5.627151916336437 2210 9 -5.045812283090257 2210 10 -2.4235702542677195 2210 11 -1.4166698149516996 2210 12 0.3591630072830476 2210 13 -7.496143126857451 2210 14 -2.1481397173470818 2210 15 -5.914858746643755 2210 16 -2.054932450759857 2210 17 -1.3882270618377213 2210 18 -0.8443283507163066 2210 19 -1.2091912451902438 2210 20 -0.9591150074849217 2210 21 -1.3688644246530757 2210 22 -1.1632796472801417 2210 23 -0.8142781205483267 2210 24 -0.8085643212010503 2210 25 -0.6535146992836403 2211 1 0.23425000431115706 2211 2 0.126782257176135 2211 3 -3.617891492776657 2211 4 -3.045719086588747 2211 5 -0.8462275434942299 2211 6 -0.9474097304739679 2211 7 -1.0553142482199824 2211 8 -4.725901012029297 2211 9 0.27175774535996666 2211 10 -0.08268303318489956 2211 11 -6.138176552350856 2211 12 -0.6639807872543884 2211 13 0.6202236631034701 2211 14 1.1734639936209312 2211 15 -4.338319782574228 2211 16 -1.0222420586562606 2211 17 -6.453846175134096 2211 18 -2.3841611521628003 2211 19 -1.4536783301418765 2211 20 -0.896405847587495 2211 21 -0.877181064999057 2211 22 -0.5742935858001914 2211 23 -0.3541596824347799 2211 24 -0.2608465088133123 2211 25 -0.015105702683936073 2212 1 2.076653546605698 2212 2 1.2271357945504207 2212 3 2.2152899074375263 2212 4 2.622642003826055 2212 5 -0.9215676267672741 2212 6 0.3343283411541158 2212 7 1.067829166912123 2212 8 -1.4605199014072063 2212 9 2.7520061695269575 2212 10 1.9653873080644537 2212 11 1.7801167719277657 2212 12 3.356834410527206 2212 13 3.444158229852576 2212 14 3.9006203174810636 2212 15 4.2961860851441145 2212 16 3.360820985117421 2212 17 2.8297821391436173 2212 18 2.353454316106607 2212 19 1.715639055801045 2212 20 1.5745872723473902 2212 21 1.3726701558907912 2212 22 1.4413011864028604 2212 23 1.5993451785238904 2212 24 1.62162943871407 2212 25 2.0040133591732108 2213 1 2.304859464723336 2213 2 1.9361590457081164 2213 3 2.398072214445147 2213 4 2.848943708483567 2213 5 1.5291608598208057 2213 6 -0.4129455284643804 2213 7 0.844942951225903 2213 8 -5.974811489629712 2213 9 0.5330290429842002 2213 10 0.7812600748039529 2213 11 1.0915024509856508 2213 12 2.8871215812966042 2213 13 -2.1760849669206763 2213 14 1.0856893543970187 2213 15 -4.029860119053197 2213 16 -0.17381195146727535 2213 17 1.2462583107768914 2213 18 1.676961069002246 2213 19 1.358954586789931 2213 20 1.6159691570315928 2213 21 1.3363251720038534 2213 22 1.3951045683693373 2213 23 1.7705793573830526 2213 24 1.7848865903505384 2213 25 2.01540674382559 2214 1 2.158229483336388 2214 2 1.467971001771101 2214 3 1.0069306010364762 2214 4 1.8192899294533404 2214 5 1.767465984512178 2214 6 0.06306468336356019 2214 7 0.4205286259936578 2214 8 1.9723311811877977 2214 9 3.106488186056442 2214 10 1.5067194654441352 2214 11 1.4754822525186846 2214 12 2.656278287258271 2214 13 0.9091951957188726 2214 14 2.457628367363217 2214 15 3.3461674365175895 2214 16 2.127604640690066 2214 17 1.469402521550922 2214 18 1.151733790791475 2214 19 0.9363209674130566 2214 20 0.42974159243926024 2214 21 0.37293288276617176 2214 22 0.20430031873273435 2214 23 -6.3912910883546346 2214 24 -2.610262441704265 2214 25 -0.916474831979329 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 255401.0 3 8.0 4 2058.0 5 11507.0 6 14933.0 7 13865.0 8 12214.0 9 10328.0 10 9202.0 11 8659.0 12 8288.0 13 7950.0 14 7591.0 15 7548.0 16 7641.0 17 8430.0 18 10302.0 19 13864.0 20 20086.0 21 28123.0 22 37052.0 23 45608.0 24 54894.0 25 63962.0 26 72063.0 27 80530.0 28 86531.0 29 100464.0 30 108248.0 31 115759.0 32 142701.0 33 172181.0 34 200352.0 35 222201.0 36 235786.0 37 221783.0 38 189562.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68917384415139 17.239648161659275 14.61308468983502 23.45809330435431 2 16.007759790950065 20.505239896489986 39.8121410894848 23.674859223075153 3 17.337553312025825 25.699539516355685 30.877779405381318 26.08512776623717 4 10.40561076293911 15.62349718576996 38.135914038064236 35.83497801322669 5 11.592532779636251 38.2631983696697 35.4554927519514 14.688776098742645 6 31.94419604309145 34.986897870312575 19.010722861398968 14.058183225197013 7 29.859361105726478 31.902675085154108 20.638832734869954 17.59913107424946 8 26.066936385549027 32.701497812877044 23.54831228261944 17.68325351895449 9 26.74672165735218 14.833175815945838 23.27072257591511 35.14937995078687 10 15.388819634967515 30.03215852100804 32.12498168222027 22.45404016180417 11 35.31690093444285 22.9091384625444 22.719942152622462 19.0540184503903 12 23.05101982337088 25.335482637674904 31.021984983332125 20.591512555622096 13 27.857001096938266 20.801211093692064 27.94365614293121 23.39813166643846 14 21.93657517645036 20.415088635754376 29.064512121623537 28.58382406617172 15 23.21410508818372 27.612080075905272 26.049822255674144 23.123992580236862 16 23.716562628605274 27.98727697201165 25.807593047719045 22.48856735166403 17 22.64336570176734 27.995733951164283 26.62234468074632 22.73855566632206 18 22.25519561735888 27.313163153692432 27.960644227322582 22.470997001626106 19 24.041152443791695 26.85788431489957 27.009906229437338 22.091057011871403 20 24.60661231431001 26.383618881993314 26.690572469595125 22.31919633410155 21 25.414182329066744 25.88777530909115 26.064959234620822 22.63308312722129 22 24.784597313706772 26.33258897836704 25.904091285662307 22.978722422263882 23 23.69002250862728 26.319131717779047 26.70449387897651 23.28635189461716 24 23.83659120106467 26.384613017624027 26.55716077019532 23.221635011115982 25 23.479053194627728 26.207382238764467 27.20113479410895 23.112429772498857 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6181.0 1 6181.0 2 3727.0 3 1273.0 4 1273.0 5 1273.0 6 1699.0 7 2125.0 8 2125.0 9 2125.0 10 2887.5 11 3650.0 12 3650.0 13 3650.0 14 5745.0 15 7840.0 16 7840.0 17 7840.0 18 14833.0 19 21826.0 20 21826.0 21 21826.0 22 37203.5 23 52581.0 24 52581.0 25 52581.0 26 77487.0 27 102393.0 28 102393.0 29 102393.0 30 132137.5 31 161882.0 32 161882.0 33 161882.0 34 195245.0 35 228608.0 36 228608.0 37 228608.0 38 258490.5 39 288373.0 40 288373.0 41 288373.0 42 312712.0 43 337051.0 44 337051.0 45 337051.0 46 349789.0 47 362527.0 48 362527.0 49 362527.0 50 347100.0 51 331673.0 52 331673.0 53 331673.0 54 299365.0 55 267057.0 56 267057.0 57 267057.0 58 229290.5 59 191524.0 60 191524.0 61 191524.0 62 155283.5 63 119043.0 64 119043.0 65 119043.0 66 91587.0 67 64131.0 68 64131.0 69 64131.0 70 47964.0 71 31797.0 72 31797.0 73 31797.0 74 22947.5 75 14098.0 76 14098.0 77 14098.0 78 10563.0 79 7028.0 80 7028.0 81 7028.0 82 5071.0 83 3114.0 84 3114.0 85 3114.0 86 2244.0 87 1374.0 88 1374.0 89 1374.0 90 906.0 91 438.0 92 438.0 93 438.0 94 257.0 95 76.0 96 76.0 97 76.0 98 44.0 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23979215201280832 2 0.2521019682284027 3 0.25747073542523513 4 0.3215508067531422 5 0.26747965141361557 6 0.2958957692197072 7 0.2946302740947396 8 0.2946686224318598 9 0.3053294601512842 10 0.29689282598483324 11 0.29643264593939045 12 0.29773648940147834 13 0.2957040275341061 14 0.2934414756440124 15 0.2911405754167985 16 0.2939400040265754 17 0.2918691938220829 18 0.2914090137766401 19 0.28347090799275215 20 0.32922047417718847 21 0.2896066419319892 22 0.2937866106780945 23 0.2984651078067628 24 0.29969225459461013 25 0.29650934261363093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2607675.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.19605949416277 #Duplication Level Percentage of deduplicated Percentage of total 1 93.00724571646904 74.58814610866168 2 4.137199039907123 6.635741206871694 3 1.2164973104952543 2.926748720608993 4 0.5302566340076621 1.7009797027221183 5 0.28902635646934755 1.1589387439398444 6 0.1765639089477191 0.849583784789593 7 0.11413086155119068 0.6406991754155539 8 0.07629710101840252 0.48949814820031634 9 0.05961898761033182 0.4303087089641931 >10 0.3405678033244033 5.074423816784906 >50 0.0326475881280198 1.8246591437959359 >100 0.018867756310524647 2.812914884453161 >500 8.459497260639055E-4 0.4635858694825565 >1k 1.879888279910629E-4 0.19063555509151747 >5k 4.699720699776573E-5 0.21313643021797773 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 5655 0.21685984641490985 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.8348337120231625E-5 0.0 0.0 0.0 3 0.0 3.8348337120231625E-5 0.0 0.0 0.0 4 0.0 3.8348337120231625E-5 0.0 0.0 0.0 5 0.0 3.8348337120231625E-5 0.0 0.0 0.0 6 0.0 3.8348337120231625E-5 0.0 0.0 0.0 7 0.0 3.8348337120231625E-5 0.0 3.8348337120231625E-5 0.0 8 0.0 3.8348337120231625E-5 0.0 3.8348337120231625E-5 0.0 9 0.0 3.8348337120231625E-5 0.0 3.8348337120231625E-5 0.0 10 0.0 3.8348337120231625E-5 0.0 3.8348337120231625E-5 0.0 11 0.0 3.8348337120231625E-5 0.0 3.8348337120231625E-5 0.0 12 0.0 3.8348337120231625E-5 0.0 3.8348337120231625E-5 0.0 13 0.0 3.8348337120231625E-5 0.0 7.669667424046325E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCGCG 575 0.0 11.731884 18 CGAGCGC 65 8.040324E-4 11.689927 10 ACATGGT 1450 0.0 11.665531 3 TGCGCGC 605 0.0 11.307182 19 GACACCG 90 9.535749E-5 10.553813 7 GGTATCA 2215 0.0 10.552703 1 GTCTTGG 470 0.0 10.512544 1 TACATGT 1735 0.0 10.35078 2 CATGGTA 1075 0.0 10.342605 4 ATTTGCG 625 0.0 10.337477 16 CTCAACG 205 3.6379788E-12 10.198148 4 TCGACGG 75 0.002645037 10.134975 14 CGACGGT 75 0.0026458816 10.134585 15 AAGTCGT 75 0.0026526481 10.131466 6 TTTGCGC 670 0.0 9.926218 17 GACCGAA 115 1.0254826E-5 9.9142685 19 GTATCAA 4840 0.0 9.815829 1 TCGTGAC 215 1.0913936E-11 9.718765 13 ATTAACC 245 0.0 9.696755 3 CCGTCTA 80 0.004523327 9.498432 9 >>END_MODULE