FastQCFastQC Report
Thu 26 May 2016
SRR1364248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607675
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG45060.1727976070637637No Hit
GTATCAACGCAGAGTACTTTTTTTT37540.1439596575493495No Hit
GGTATCAACGCAGAGTACATGGGAT29080.11151696434563356No Hit
TATCAACGCAGAGTACTTTTTTTTT28160.10798891733057225No Hit
GGTATCAACGCAGAGTACTTTTTTT27280.10461426366399186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAC601.4987563E-615.8016017
TAAGGCG704.540325E-714.8989385
TCGACCG653.212579E-614.68468818
CGCGCTG500.001527497313.26799910
GTTCGAC801.8973606E-613.1244416
CGACCGT751.409244E-512.72672919
AAGTTCG1001.3599674E-712.40688314
GGTATCA33950.011.50495151
CGCGGTT751.985637E-411.45405718
TATACCG855.434706E-511.1542855
TTCGACC951.2915229E-511.0521617
TGGTATC12250.010.9136162
TATGACG700.001516922310.8355914
CGACGGG1501.9790605E-910.81625714
ACACCGT803.8430962E-410.666086
CCGAGTA1252.3169196E-710.6146039
CGGTGAT1803.6379788E-1110.52975611
ACGCCGA1451.49048E-810.4617496
AAACCGT1002.4538025E-510.4290576
GTGGTAT13900.010.2988671
Produced by FastQC (version 0.11.5)