FastQCFastQC Report
Thu 26 May 2016
SRR1364247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276136
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65090.5100553546017039No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA46270.3625789100848186No Hit
TATCAACGCAGAGTACTTTTTTTTT43360.3397756978879994No Hit
GGTATCAACGCAGAGTACTTTTTTT38400.3009083671332836No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29220.228972460615483No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN27100.21235981117999964No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18020.1412075202016086No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17420.13650582696515104No Hit
AAACAAAAAAAAAAAAAAAAAAAAA13680.10719860579123228No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA13310.10429922829541678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT702.4934707E-816.28999519
AAGTTCG651.905064E-716.08241514
GTTCGAC807.683411E-915.44155816
AGTTCGA755.8236765E-815.20399615
TCGACCG801.2836426E-714.25374618
CGCACGG400.00528710814.24646211
ATAACGC1051.364242E-913.5749973
TATAACG1205.0931703E-1113.4597542
CGGCAGC500.00149543213.304543514
GATCGCC500.001502093913.29669911
TTCGACC957.3572664E-813.00341817
ACATGGT8850.012.5626243
GTATTAG1457.2759576E-1212.4495911
TTGCGCG4000.012.115684518
GGTATCA25200.012.0270621
TCGCACG658.0039917E-411.69538118
TAACGCA1302.6011548E-911.6953814
ATCCGAC658.0039917E-411.69538116
TAGCACC658.0039917E-411.6953814
TGCGCGC4150.011.67776819
Produced by FastQC (version 0.11.5)