FastQCFastQC Report
Thu 26 May 2016
SRR1364247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276136
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT226161.772224903928735No Hit
TATCAACGCAGAGTACTTTTTTTTT176541.383394873273695No Hit
GGTATCAACGCAGAGTACTTTTTTT157391.2333324974767579No Hit
GTACTTTTTTTTTTTTTTTTTTTTT93190.7302513211757995No Hit
ACGCAGAGTACTTTTTTTTTTTTTT76570.6000144185259251No Hit
GAGTACTTTTTTTTTTTTTTTTTTT65490.5131898167593423No Hit
GTGGTATCAACGCAGAGTACATGGG30790.2412752245842136No Hit
GTGGTATCAACGCAGAGTACTTTTT30640.24009980127509922No Hit
ATCAACGCAGAGTACTTTTTTTTTT28950.2268566986590771No Hit
GCAGAGTACTTTTTTTTTTTTTTTT25900.20295642470708453No Hit
GGTATCAACGCAGAGTACATGGGAT20940.1640890939523687No Hit
GTATAACGCAGAGTACTTTTTTTTT15220.11926628509814001No Hit
GGTATCAACGCAGAGTACATGGGAA14050.11009798328704778No Hit
GGTATCAACGCAGAGTACATGGGAG13530.10602318248211788No Hit
GGTCGGGAGTGGGTAATTTGCGCGC13090.1025752741087157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2050.016.6568012
ATAACGC2050.016.6554973
TAACGCA2000.016.1234464
TATCACG1055.456968E-1215.3568672
AAGTTCG902.1682354E-914.83371814
GCGCCGA602.4748964E-514.305634519
TTAGTAC852.752313E-713.3897133
TTAGTCT500.001517996313.2781324
TTCAACG957.5353455E-812.9796422
GGTATCA46050.012.8507911
TCGACCG905.152997E-712.71611918
AGTTCGA1001.3702629E-712.39821615
ACGCCGA1700.012.2739046
CGGCTTA701.0527982E-412.26052314
CCGGCTT550.00311509712.066298513
CGACCGT959.91482E-712.0468519
GTATAAC2900.011.772791
TGGGCCC1001.967046E-611.3812565
TAGGACA1251.8750143E-811.3812564
CGCAAGT752.1067189E-411.3803641
Produced by FastQC (version 0.11.5)