FastQCFastQC Report
Thu 26 May 2016
SRR1364246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813223
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN36990.20400138317239522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCG801.2918827E-714.2472225
TAACGCA707.235223E-613.5766654
CGCGCCA1301.4551915E-1113.15091610
TCGCGGT1251.0186341E-1012.92141117
ACATGGT8600.012.708043
AGGACCG1051.999615E-812.6641965
GGTATCA12150.011.9659241
GACCTAC1900.011.0027231
TTGCGCG2700.010.90889918
TGCGCGC2800.010.85892919
CTCGACG700.0014950710.85412413
ACGGCCC1502.1300366E-910.76724615
GAACTGT1951.8189894E-1210.7158586
TTTGCGC2750.010.7102617
CAAGACG909.4789735E-510.5596294
CGACCTA909.5029325E-510.55718
ATTTGCG2700.010.55670816
CGCCAGT1451.4495527E-810.47984512
TACATGT10100.010.1612112
GTATTAA2450.010.0841281
Produced by FastQC (version 0.11.5)