FastQCFastQC Report
Thu 26 May 2016
SRR1364246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813223
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG23160.12772835994248913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCCG603.9058426E-412.736979519
GCTTATA3200.012.7338041
CGCGCCA1900.012.46540210
CGCCAGT1600.012.43389612
CCAAGAC3450.012.08674053
CGAGTAT907.0162714E-611.67556518
ACGGATC658.18673E-411.6644498
GTGCACG3250.011.659945511
ACCGTGC907.66314E-611.5834468
CAAGACT2700.011.5831264
ACGGCCC3150.011.52393415
GCCCCCG3000.011.46328218
GCGAGTA1001.8055998E-611.46328217
GGCGAGT1104.6260357E-711.28959616
CCTATAA4050.011.2311931
ACCAGTT3600.011.05235212
CAGTTGA3450.010.79814314
GGCCCCC3300.010.71064217
TATAAGC4100.010.6328483
TCGTGAC1803.8198777E-1110.5263413
Produced by FastQC (version 0.11.5)