FastQCFastQC Report
Thu 26 May 2016
SRR1364245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333786
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN23630.1771648525325652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGT250.006038459518.99627713
AGCCCGT400.005282802514.248286
ATCGTGC400.00528543814.2472098
TCGCCAG604.0981034E-412.66418613
GGTATCA14700.012.5396731
ACAACCG550.00306995612.0899055
AGGACCG550.00306995612.0899055
ACATGGT8050.011.6862133
AAAGGTA1001.930628E-611.3990525
GTCTTAG1354.7093636E-911.2612871
TAACTCC600.005863748511.0861634
CGATTCG600.005883476711.0811629
TTGCGCG4400.011.01251918
GAATAGG951.3569632E-511.0019811
GCCTTAC700.001488918410.8590981
TACATGT8950.010.8283592
GTATTAG1855.456968E-1210.7857261
CTATACT803.761019E-410.6902294
TGCGCGC4650.010.62437119
TTTGCGC4750.010.60111217
Produced by FastQC (version 0.11.5)