##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364245_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1333786 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.56690728497675 29.0 23.0 31.0 7.0 33.0 2 25.172453452053027 30.0 25.0 31.0 7.0 34.0 3 24.8209840259232 30.0 23.0 31.0 7.0 34.0 4 27.956463030801043 33.0 26.0 35.0 7.0 37.0 5 28.366288145174714 33.0 27.0 35.0 7.0 37.0 6 29.070584036719534 35.0 28.0 35.0 2.0 37.0 7 29.16159263929896 35.0 29.0 35.0 2.0 37.0 8 27.325271820217036 33.0 23.0 35.0 2.0 37.0 9 27.113556447586046 32.0 17.0 37.0 2.0 39.0 10 28.830767454449216 34.0 27.0 37.0 2.0 39.0 11 29.430909456239608 35.0 27.0 38.0 2.0 39.0 12 28.06008535102333 34.0 23.0 37.0 2.0 39.0 13 27.74819423805618 33.0 19.0 37.0 2.0 39.0 14 28.166903086402165 33.0 19.0 38.0 2.0 40.0 15 27.605306248528624 33.0 17.0 38.0 2.0 40.0 16 28.467934136360707 34.0 24.0 38.0 2.0 40.0 17 29.059152667669327 34.0 26.0 39.0 2.0 40.0 18 29.631814998807904 35.0 27.0 39.0 2.0 40.0 19 30.31924761543456 36.0 28.0 40.0 2.0 41.0 20 30.36388296173449 36.0 29.0 40.0 2.0 41.0 21 30.51061789522457 36.0 29.0 40.0 2.0 41.0 22 30.415593655953803 36.0 29.0 40.0 2.0 41.0 23 30.008314677167103 36.0 28.0 40.0 2.0 41.0 24 29.98262090020438 36.0 28.0 40.0 2.0 41.0 25 29.671651224409313 36.0 27.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.3560631341058702 1101 2 -0.7251629236906929 1101 3 -3.146140439084757 1101 4 -3.140355155694369 1101 5 -4.12706277590042 1101 6 -2.0890183983922412 1101 7 -1.5284085124870082 1101 8 -5.637075744464397 1101 9 -6.3180146055861215 1101 10 -6.906401888615651 1101 11 -3.698760599384098 1101 12 -0.8734649884141383 1101 13 -0.25115043787868885 1101 14 -7.594350703210548 1101 15 -9.314410870017895 1101 16 -4.430697872474042 1101 17 -2.4689992214639425 1101 18 -7.336528640578443 1101 19 -3.85720904753612 1101 20 -2.827893766071668 1101 21 -2.652742650049266 1101 22 -2.610448637598278 1101 23 -7.641749334793644 1101 24 -4.418998185278539 1101 25 -3.386110215685367 1102 1 1.9165048482497262 1102 2 1.4936881992337625 1102 3 1.8842552569546633 1102 4 2.087227679137886 1102 5 1.6308496970429758 1102 6 1.15847863955819 1102 7 0.7882234260085355 1102 8 2.232096076128304 1102 9 -3.6292267776190634 1102 10 -0.47960372544834584 1102 11 0.3701366132729653 1102 12 -4.360413268977542 1102 13 -6.267257713541138 1102 14 -6.1296370990175895 1102 15 -0.8986618756669031 1102 16 0.7824740784247091 1102 17 -4.025547221145416 1102 18 -1.3214665280277984 1102 19 -0.3853264991358678 1102 20 -0.08426156937709806 1102 21 0.15858987409957948 1102 22 0.18739359073170547 1102 23 0.4190299919101683 1102 24 0.4170228108870866 1102 25 0.7038962754863967 1103 1 -0.7664545712101294 1103 2 1.1146822471353666 1103 3 1.9623035444439267 1103 4 2.706842500123205 1103 5 2.480890352616008 1103 6 1.5238831203995389 1103 7 1.39275770914589 1103 8 3.0680869006784484 1103 9 4.2530780182072725 1103 10 2.696815735938049 1103 11 1.9674000567923429 1103 12 3.408747143672695 1103 13 3.523289214934149 1103 14 -2.7546705192767753 1103 15 1.6946628008112086 1103 16 2.3808273363066057 1103 17 2.4617585929843493 1103 18 2.241954787742735 1103 19 1.853993182295941 1103 20 1.78776326191376 1103 21 1.627508190851664 1103 22 1.680214168729922 1103 23 2.0999976711124297 1103 24 1.7098812115841255 1103 25 1.8581226941055782 1104 1 1.4013803708816752 1104 2 0.7544529052451594 1104 3 1.4677082303430176 1104 4 1.7956860978332614 1104 5 1.1429396889290793 1104 6 0.4741658874483079 1104 7 0.17070069439808222 1104 8 1.5130070078289535 1104 9 -3.819764416678101 1104 10 -1.2532071334740529 1104 11 -0.3344069822468114 1104 12 1.2077707501197352 1104 13 1.1497758649642051 1104 14 0.9010731789954995 1104 15 -4.058485475925266 1104 16 -5.0102018504349815 1104 17 -1.749209877245523 1104 18 -1.060113728857928 1104 19 -0.8333626001894849 1104 20 -0.5378401311328354 1104 21 -0.9763523555021436 1104 22 -0.9315287806579775 1104 23 -1.2031777408332225 1104 24 -7.1629713348202415 1104 25 -4.092454412941439 1105 1 -2.3614869043576086 1105 2 0.1282274708670279 1105 3 1.1269555612873141 1105 4 1.732254670978783 1105 5 1.5974875032150102 1105 6 1.447756206314697 1105 7 0.6652528773205333 1105 8 3.030666437349673 1105 9 3.383455542701416 1105 10 2.060053980224609 1105 11 1.8836731979055727 1105 12 2.327923926662116 1105 13 2.2624489758594954 1105 14 2.969890468609165 1105 15 3.462785914843881 1105 16 3.101352164670118 1105 17 1.7729739316667903 1105 18 2.108447564532746 1105 19 1.0050620067924 1105 20 0.9912605223505402 1105 21 0.9217687879639804 1105 22 0.9393515632692981 1105 23 1.4383726941448636 1105 24 1.539607287168792 1105 25 1.549982972792023 1106 1 2.401446140392423 1106 2 1.7418211731502886 1106 3 2.3415194588925665 1106 4 2.719421367580434 1106 5 1.6760937505202307 1106 6 1.063641569919092 1106 7 1.117510100833229 1106 8 3.008664476230937 1106 9 3.884679858360027 1106 10 1.9995999042621975 1106 11 1.0417233689374648 1106 12 2.264287686516134 1106 13 -3.9700452220364006 1106 14 0.5699565771267494 1106 15 2.69872288736558 1106 16 2.2178374857114953 1106 17 1.7225503390763777 1106 18 1.5235119549332232 1106 19 1.4848362718634078 1106 20 0.8379264947268403 1106 21 0.5057620744779925 1106 22 0.6047951720497586 1106 23 1.1545799971739292 1106 24 1.013294940019584 1106 25 0.998371288388249 1107 1 1.9967755828133988 1107 2 1.3739859441638949 1107 3 2.13896859876132 1107 4 2.124757301820811 1107 5 1.7177077408089723 1107 6 0.551941145094986 1107 7 0.052892815179941266 1107 8 2.022201870016204 1107 9 3.4757147393514636 1107 10 1.8461928832062746 1107 11 0.6069245352360078 1107 12 2.2342741665360286 1107 13 2.4321868981207686 1107 14 -3.7660650799171194 1107 15 0.7407260733722261 1107 16 1.0657382319224524 1107 17 0.685777351453325 1107 18 0.7846927555863061 1107 19 0.454959100987157 1107 20 0.5750940066534689 1107 21 0.17999115351032202 1107 22 -0.0015909114954979486 1107 23 0.6897915002321184 1107 24 0.8135934801264177 1107 25 0.8746826254592541 1108 1 1.2414278087972512 1108 2 0.6014334466891462 1108 3 0.9174424675847632 1108 4 1.4423764360333529 1108 5 0.6713252043082925 1108 6 -0.11826454016026133 1108 7 -0.3991310066902045 1108 8 -4.669324943084117 1108 9 -6.05347728043569 1108 10 -2.774452571842872 1108 11 -1.1701648862795402 1108 12 -0.0055662348897591585 1108 13 1.3317484069339223 1108 14 1.6939010227891238 1108 15 -4.194863385791194 1108 16 -0.6175873824127223 1108 17 -0.030158425534875022 1108 18 -0.13016409014690922 1108 19 -0.3476932272852622 1108 20 -0.28371971592573786 1108 21 -0.5054842965238464 1108 22 -0.5646454185143419 1108 23 -0.18645709507595853 1108 24 -0.2700167663464015 1108 25 0.13459786801811546 1109 1 0.44948117266903864 1109 2 0.43651230405738417 1109 3 0.6157876478314108 1109 4 -1.4391537965167416 1109 5 -3.1293281779004047 1109 6 -1.4429875403557375 1109 7 -1.0946609520773478 1109 8 1.3069794427244972 1109 9 -4.326342111450671 1109 10 -1.1219745804612202 1109 11 -0.562284512137353 1109 12 -7.093037972692688 1109 13 -2.0010331468252325 1109 14 0.04059821637673977 1109 15 1.5060996480334872 1109 16 1.2635728251057579 1109 17 0.8006055490079582 1109 18 0.36686533021640244 1109 19 -0.23873443516874815 1109 20 -0.284227959751302 1109 21 -0.22597994669709465 1109 22 -0.376859531362463 1109 23 -0.16657244462765775 1109 24 -0.26729269707439585 1109 25 0.07169361264375951 1110 1 0.3981577810939996 1110 2 0.43740351939866073 1110 3 -3.0956596788968156 1110 4 -2.917223462011588 1110 5 -0.5979823645582911 1110 6 -0.7687605133107276 1110 7 -0.9167592003352105 1110 8 -4.993677325430607 1110 9 -8.302794765011917 1110 10 -3.424563313190596 1110 11 -1.3816533926208514 1110 12 0.9983551791873388 1110 13 1.4757723834337568 1110 14 1.8720625423972166 1110 15 2.3839683825592637 1110 16 1.8361197997889533 1110 17 1.2697670530960217 1110 18 0.7615245251784906 1110 19 -0.09286853164056197 1110 20 -0.03910332183872356 1110 21 -0.38540286896192555 1110 22 -0.542916502278203 1110 23 -0.2522445528975119 1110 24 -0.18531302802832528 1110 25 0.010519447243307667 1111 1 0.8692446986106219 1111 2 0.7825102038493696 1111 3 1.0111479605885023 1111 4 1.7449399704136788 1111 5 -2.1017995675826313 1111 6 -0.9040313571159331 1111 7 -1.0844814645548588 1111 8 1.3240115277494766 1111 9 2.878126014214004 1111 10 1.47472829361325 1111 11 1.077188362405316 1111 12 2.3397274461115316 1111 13 2.626192973341446 1111 14 2.8000180506342076 1111 15 3.0861031929705796 1111 16 2.1377942766571714 1111 17 1.5727787961034494 1111 18 0.8329230591825514 1111 19 0.4708962559602128 1111 20 0.4954528621390182 1111 21 0.25353098575513044 1111 22 0.17428906169882552 1111 23 0.4874319965765501 1111 24 0.5792244022306647 1111 25 0.4270559988546232 1112 1 2.327954536609905 1112 2 1.6045867240264116 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3.7616648433886795 2204 9 -2.5610152171367844 2204 10 0.4073988509784314 2204 11 1.8742348999852396 2204 12 3.597730019803574 2204 13 3.718642303779376 2204 14 4.162772906467243 2204 15 4.539788046390775 2204 16 4.175826608663446 2204 17 3.2372940170790407 2204 18 2.9873483517389516 2204 19 2.3567226021217955 2204 20 2.304409008839464 2204 21 2.0009560370279047 2204 22 1.9169839484965898 2204 23 2.473912573244423 2204 24 2.438987089728151 2204 25 2.3423780119643673 2205 1 -3.1295924247447857 2205 2 -3.762025741464207 2205 3 -0.2530811891925602 2205 4 -2.2627388385967357 2205 5 -2.842666684871965 2205 6 -0.9246955157922052 2205 7 -0.5228579230889068 2205 8 -4.906588503855279 2205 9 1.0567903818894742 2205 10 0.7910549542648155 2205 11 0.7573367518370944 2205 12 2.3923092234203374 2205 13 -4.2961268667996535 2205 14 0.10415266650897337 2205 15 2.494942584731934 2205 16 2.185542310280553 2205 17 2.120401302768485 2205 18 0.7318547419319614 2205 19 0.9363011536780448 2205 20 0.9276810484847431 2205 21 0.829708645269946 2205 22 0.8491098831166539 2205 23 1.0189373515438511 2205 24 0.7047723879371581 2205 25 1.453152225144681 2206 1 -7.663963208836307 2206 2 -6.164363041124236 2206 3 -2.8599629009188554 2206 4 -1.328571767202856 2206 5 -1.1327596372220796 2206 6 -1.51194863219175 2206 7 -2.1631768481334284 2206 8 -3.4472804524819054 2206 9 -6.212712748000982 2206 10 -3.0763415704775063 2206 11 -2.1878739761322556 2206 12 -7.628696118978887 2206 13 -7.046491795837479 2206 14 -7.96819771933097 2206 15 -2.7824102294712922 2206 16 -6.622369432205012 2206 17 -3.154558899055484 2206 18 -2.2956417504321855 2206 19 -2.5006821445789313 2206 20 -2.51696710865734 2206 21 -2.3733335542875516 2206 22 -2.4727217773441943 2206 23 -1.980653199424797 2206 24 -1.9783208072492862 2206 25 -2.1904072157921277 2207 1 -7.262015520903905 2207 2 -5.7453527455186695 2207 3 -1.5171486320025593 2207 4 -0.3315920849583307 2207 5 0.18066942837993238 2207 6 -0.3200343742136802 2207 7 -0.4510094385788861 2207 8 1.076781936426734 2207 9 -2.20970917940463 2207 10 -1.0268932672698732 2207 11 -0.7363631336153702 2207 12 -4.743752587572505 2207 13 -5.0016249003795785 2207 14 -1.2751988678447397 2207 15 -5.3539223025386455 2207 16 -1.4997358491155097 2207 17 -0.6101643982487914 2207 18 -0.17496679350145072 2207 19 -0.3595718462388078 2207 20 -0.4755734418904076 2207 21 -0.6641420259438711 2207 22 -0.5733018451611969 2207 23 -0.6984172208637212 2207 24 -0.7921973146736079 2207 25 -0.16460804162821674 2208 1 -4.104788599818161 2208 2 -4.339185616491356 2208 3 -1.4376973877679582 2208 4 -0.09739207235277192 2208 5 -3.011751776680228 2208 6 -1.2214322429960767 2208 7 -0.7989092488369884 2208 8 -5.050848886236558 2208 9 -8.364615674058712 2208 10 -3.8544264752374637 2208 11 -1.448871235509685 2208 12 -0.02982918234044618 2208 13 1.051555510580485 2208 14 1.0252844393524398 2208 15 1.3308739499099538 2208 16 -4.822113947551134 2208 17 -7.80337862472053 2208 18 -3.4981490451241015 2208 19 -2.778131149910191 2208 20 -1.6369133188262772 2208 21 -1.5280038614827092 2208 22 -1.0455904660721913 2208 23 -0.7346102289171696 2208 24 -0.7732083871841127 2208 25 -0.4497362404849987 2209 1 0.9914727566129216 2209 2 0.9658085225022361 2209 3 -2.18690808217438 2209 4 0.3015887442573906 2209 5 0.7862917468491979 2209 6 0.22726020273989533 2209 7 0.25071487464423825 2209 8 1.7788885266380028 2209 9 -3.9223527874487125 2209 10 -0.6623291240380063 2209 11 0.23827034815530368 2209 12 -4.458451951121987 2209 13 0.1517373070666892 2209 14 1.5519631321959295 2209 15 2.4915015572389017 2209 16 -3.0936397511875313 2209 17 -0.19941794142778946 2209 18 0.43679635743695755 2209 19 0.3456986960658348 2209 20 0.44821516706869957 2209 21 0.5052473475692132 2209 22 0.3793364760734761 2209 23 0.7462197176669534 2209 24 0.22641632122789446 2209 25 0.2418072982526276 2210 1 -0.36930102937504117 2210 2 -3.1145663465641285 2210 3 -0.9157144067603085 2210 4 -3.269302251901891 2210 5 -3.6904537459121656 2210 6 -1.5289051817598747 2210 7 -0.7078636196363028 2210 8 -5.4201643578328635 2210 9 -4.658604697981442 2210 10 -1.859571482485162 2210 11 -0.8750066114558592 2210 12 0.7275785572820439 2210 13 -7.256062984744787 2210 14 -1.6993531410339529 2210 15 -5.421390636688553 2210 16 -1.5425475392241879 2210 17 -0.8564455802752491 2210 18 -0.33932412231775544 2210 19 -0.6946832023735965 2210 20 -0.5669627292526727 2210 21 -0.8580315774916585 2210 22 -0.6380293126152452 2210 23 -0.2732895673486908 2210 24 -0.34130405771080774 2210 25 -0.23468569202880118 2211 1 0.47234392829280125 2211 2 0.38063495406421666 2211 3 -3.4953879209669694 2211 4 -2.640801117643914 2211 5 -0.6009933982485443 2211 6 -0.7111567316304033 2211 7 -0.9155826219906835 2211 8 -4.96647652305127 2211 9 0.15076960965739872 2211 10 0.16569932767806606 2211 11 -6.174321450069705 2211 12 -0.45347558970863844 2211 13 0.6768141384047013 2211 14 1.3877164747347877 2211 15 -4.0906500049323995 2211 16 -0.7213970986043705 2211 17 -6.2358439832575705 2211 18 -2.193687059173161 2211 19 -1.3992086080913602 2211 20 -0.9205958175587057 2211 21 -0.7524233698448874 2211 22 -0.5513805584187743 2211 23 -0.44255175596048346 2211 24 -0.32150444798705635 2211 25 0.0568662405029805 2212 1 1.9678400686759971 2212 2 1.1055239177059626 2212 3 2.170805032842104 2212 4 2.501549345773739 2212 5 -1.3820584662719746 2212 6 0.313256750447767 2212 7 0.9082317359640371 2212 8 -1.7986937361646582 2212 9 2.5434722291109644 2212 10 2.0925970407412287 2212 11 1.870320522124235 2212 12 3.35374680437841 2212 13 3.4168580757311062 2212 14 3.7998411544905473 2212 15 4.325875749262188 2212 16 3.3541973951802966 2212 17 2.543335076865418 2212 18 2.3342194503656692 2212 19 1.7120553459142762 2212 20 1.5458519967712157 2212 21 1.2189257623712244 2212 22 1.194531978736567 2212 23 1.4081354196863707 2212 24 1.639600038819566 2212 25 2.0865432914760866 2213 1 1.9650953232677644 2213 2 1.5363788631377169 2213 3 2.0247487037925858 2213 4 2.416975986988554 2213 5 1.2291916243022492 2213 6 -0.8842053499318325 2213 7 0.2294459413281693 2213 8 -6.505187125929197 2213 9 0.05361693801233969 2213 10 0.3127304135114706 2213 11 0.23270412682387942 2213 12 2.008988844438548 2213 13 -2.7159900569352864 2213 14 0.41112985047118755 2213 15 -4.0142378812971415 2213 16 -0.6321560885913762 2213 17 0.5484115888214127 2213 18 0.7734988873840152 2213 19 0.5734389715994226 2213 20 0.8178199578474654 2213 21 0.4145425243239522 2213 22 0.6032325682708191 2213 23 0.7592074423285418 2213 24 0.8189445229780823 2213 25 1.307491337310008 2214 1 2.8335694903313495 2214 2 1.976050157448892 2214 3 1.537629251280908 2214 4 2.535221336245421 2214 5 2.1545025506495 2214 6 0.750848632968772 2214 7 1.0059670282042674 2214 8 2.630528703285041 2214 9 3.5453580011072248 2214 10 2.100204208357212 2214 11 2.04244707024111 2214 12 3.1602456170496502 2214 13 1.0612816881101992 2214 14 2.9366477027855566 2214 15 4.174634954833106 2214 16 2.71744923604707 2214 17 1.96751303911336 2214 18 1.639153136607053 2214 19 1.5574183310453265 2214 20 1.181635745088201 2214 21 0.9605844005281448 2214 22 0.8738745365678149 2214 23 -5.683046183967008 2214 24 -1.9163112947641494 2214 25 -0.5351753489669164 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 126729.0 3 3.0 4 962.0 5 5928.0 6 7752.0 7 7331.0 8 6615.0 9 5459.0 10 4831.0 11 4706.0 12 4273.0 13 4118.0 14 3774.0 15 3786.0 16 3832.0 17 4218.0 18 5099.0 19 6976.0 20 10265.0 21 14397.0 22 19123.0 23 23770.0 24 28079.0 25 32943.0 26 37172.0 27 41479.0 28 44849.0 29 51948.0 30 54886.0 31 61053.0 32 74620.0 33 89738.0 34 103571.0 35 114307.0 36 120929.0 37 112188.0 38 92077.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.23531378301323 17.19148520578922 14.250598191714339 23.32260281948321 2 16.212621988740484 21.000734054966216 38.899503592511586 23.887140363781715 3 17.217920540997465 26.36197990044144 30.88584577815347 25.534253780407628 4 10.727260697486978 15.993600153981259 37.55383329122908 35.72530585730268 5 12.048091373609859 38.71107604448452 33.98707544334235 15.25375713856327 6 31.546245153163415 34.670091927363025 19.071226231804868 14.712436687668687 7 30.215003818147927 32.40472668674953 19.97763226725833 17.40263722784422 8 26.55744210394196 32.48357234818279 22.699884482210486 18.25910106566476 9 27.16693838190008 15.426510236862024 22.76206990635408 34.64448147488382 10 16.0688731488218 30.24335139165309 30.7186599965126 22.969115463012514 11 34.93227952798218 23.342650331481206 22.03844595777814 19.686624182758468 12 23.776543333285733 26.076859076160847 29.782798063604037 20.363799526949382 13 28.194947069367878 21.094914375260707 27.419983991161317 23.2901545642101 14 22.518443140414377 20.828798956241506 28.405141833311287 28.247616070032826 15 23.327498727985887 27.458594719394064 26.14253923327424 23.07136731934581 16 24.04023277469511 28.210516347664928 25.097008245419023 22.652242632220943 17 22.831341237584475 28.33798790312883 25.9774596570549 22.853211202231787 18 22.590502702605203 27.043413038642143 27.658488705575362 22.70759555317729 19 24.340948835279857 26.636871995388766 26.872017933917153 22.150161235414227 20 25.085560821865077 25.841774131459914 26.528215523048793 22.544449523626213 21 26.13447367531067 25.40111754515837 25.70504172945186 22.7593670500791 22 25.479367632303813 25.896637117593784 25.594432398510396 23.029562851592004 23 24.20176985297323 26.04858602666955 26.36200062231963 23.387643498037587 24 24.30083556247703 26.115367299495446 26.185341992858223 23.398455145169304 25 23.868423485412748 25.93574949774936 26.8078956130389 23.38793140379899 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2793.0 1 2793.0 2 1843.0 3 893.0 4 893.0 5 893.0 6 1219.0 7 1545.0 8 1545.0 9 1545.0 10 1890.0 11 2235.0 12 2235.0 13 2235.0 14 3240.0 15 4245.0 16 4245.0 17 4245.0 18 7697.0 19 11149.0 20 11149.0 21 11149.0 22 18651.0 23 26153.0 24 26153.0 25 26153.0 26 38420.5 27 50688.0 28 50688.0 29 50688.0 30 65436.0 31 80184.0 32 80184.0 33 80184.0 34 95975.5 35 111767.0 36 111767.0 37 111767.0 38 126041.5 39 140316.0 40 140316.0 41 140316.0 42 153752.0 43 167188.0 44 167188.0 45 167188.0 46 175628.5 47 184069.0 48 184069.0 49 184069.0 50 177609.0 51 171149.0 52 171149.0 53 171149.0 54 154827.5 55 138506.0 56 138506.0 57 138506.0 58 119971.0 59 101436.0 60 101436.0 61 101436.0 62 83525.5 63 65615.0 64 65615.0 65 65615.0 66 51394.0 67 37173.0 68 37173.0 69 37173.0 70 28259.0 71 19345.0 72 19345.0 73 19345.0 74 14212.0 75 9079.0 76 9079.0 77 9079.0 78 6914.5 79 4750.0 80 4750.0 81 4750.0 82 3486.0 83 2222.0 84 2222.0 85 2222.0 86 1587.5 87 953.0 88 953.0 89 953.0 90 614.5 91 276.0 92 276.0 93 276.0 94 160.5 95 45.0 96 45.0 97 45.0 98 28.5 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20175650366700504 2 0.21165314375769426 3 0.21825090381815374 4 0.28145444621550986 5 0.2238739947787726 6 0.245841536798257 7 0.2472660531749471 8 0.24404214769085894 9 0.25108975502816794 10 0.24606646043668173 11 0.24314245313715993 12 0.24539168952140747 13 0.2452417404291243 14 0.24066829311448762 15 0.2413430640297619 16 0.24209280949117773 17 0.23946870037622225 18 0.2412680894836203 19 0.23414550760016972 20 0.2802548534772445 21 0.23924377673779754 22 0.24224275858346092 23 0.24591651134439857 24 0.2477908749979382 25 0.24554163861369066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1333786.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.19869095104755 #Duplication Level Percentage of deduplicated Percentage of total 1 90.69450325892095 69.10802424785291 2 5.338241725090687 8.135340628643444 3 1.651351370298069 3.7749243815079443 4 0.755664537060247 2.3032259408848073 5 0.4070197950666812 1.5507187787622376 6 0.24645704316137415 1.126782243873752 7 0.1625139344209364 0.8668344344925818 8 0.11678932973164197 0.71193552340811 9 0.09043817166379726 0.6202143263508726 >10 0.4819174555664222 6.789053994627388 >50 0.037149843435497196 1.9233965082580473 >100 0.01707059906884325 2.380806247586054 >500 4.905202860049367E-4 0.2798790035446801 >1k 3.924162288039494E-4 0.42886374020721946 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 2363 0.1771648525325652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 7.497454614158494E-5 0.0 11 0.0 0.0 0.0 7.497454614158494E-5 0.0 12 0.0 0.0 0.0 7.497454614158494E-5 0.0 13 0.0 0.0 0.0 7.497454614158494E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCCGT 25 0.0060384595 18.996277 13 AGCCCGT 40 0.0052828025 14.24828 6 ATCGTGC 40 0.005285438 14.247209 8 TCGCCAG 60 4.0981034E-4 12.664186 13 GGTATCA 1470 0.0 12.539673 1 ACAACCG 55 0.003069956 12.089905 5 AGGACCG 55 0.003069956 12.089905 5 ACATGGT 805 0.0 11.686213 3 AAAGGTA 100 1.930628E-6 11.399052 5 GTCTTAG 135 4.7093636E-9 11.261287 1 TAACTCC 60 0.0058637485 11.086163 4 CGATTCG 60 0.0058834767 11.081162 9 TTGCGCG 440 0.0 11.012519 18 GAATAGG 95 1.3569632E-5 11.001981 1 GCCTTAC 70 0.0014889184 10.859098 1 TACATGT 895 0.0 10.828359 2 GTATTAG 185 5.456968E-12 10.785726 1 CTATACT 80 3.761019E-4 10.690229 4 TGCGCGC 465 0.0 10.624371 19 TTTGCGC 475 0.0 10.601112 17 >>END_MODULE