FastQCFastQC Report
Thu 26 May 2016
SRR1364245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333786
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38110.2857279953455802No Hit
GTGGTATCAACGCAGAGTACATGGG32020.24006849674535496No Hit
TATCAACGCAGAGTACTTTTTTTTT31260.2343704312385945No Hit
GGTATCAACGCAGAGTACTTTTTTT29300.21967542019484385No Hit
GGTATCAACGCAGAGTACATGGGAT20710.1552722850592224No Hit
GGTATCAACGCAGAGTACATGGGAG16820.12610718661014583No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14490.10863811735915656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT653.4570476E-614.58002910
ATAACGC400.00534941814.2213943
AAGTTCG1152.3646862E-1114.10894714
GTTCGAC954.5838533E-914.06632816
TCGACCG1051.2769306E-913.63572618
AGACCCG707.412951E-613.5441845
AGTTCGA1204.7293724E-1113.52209515
TAATACC1205.2750693E-1113.4313154
AACGCCG852.7648457E-713.384845
CCGAGTA852.778761E-713.3793219
ACGCCGA1001.0353688E-813.27336
CCCCCGC751.40861885E-512.72619619
CCTCTAG1650.012.6398161
TGCACGG1052.060915E-812.63602512
ACGGCCC853.7391546E-612.35236515
GGTATCA25550.012.2440481
CGACCGT1103.5706762E-812.14773419
CGGTTTG550.002962994412.14727514
TAACGCA658.158474E-411.6688354
TGTACCG658.158474E-411.6688355
Produced by FastQC (version 0.11.5)