##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364245_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1333786 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.301146510759597 31.0 29.0 33.0 25.0 34.0 2 29.341742978258882 31.0 28.0 34.0 25.0 34.0 3 29.410376927033273 31.0 28.0 34.0 25.0 34.0 4 32.954569173765506 35.0 32.0 37.0 27.0 37.0 5 32.310757497829485 35.0 32.0 37.0 25.0 37.0 6 32.33079144630398 35.0 32.0 37.0 25.0 37.0 7 31.92240509347077 35.0 32.0 37.0 21.0 37.0 8 31.800132854895764 35.0 32.0 37.0 19.0 37.0 9 32.283552234016554 37.0 31.0 39.0 15.0 39.0 10 32.02519519623088 37.0 30.0 39.0 15.0 39.0 11 32.68912329264215 37.0 32.0 39.0 17.0 39.0 12 32.54486776739297 37.0 32.0 39.0 17.0 39.0 13 32.70572490639428 37.0 32.0 39.0 17.0 39.0 14 33.21979238048682 38.0 32.0 40.0 16.0 41.0 15 33.41418188524996 38.0 32.0 40.0 17.0 41.0 16 33.47259830287617 38.0 32.0 40.0 17.0 41.0 17 33.40979812353706 38.0 32.0 40.0 17.0 41.0 18 33.33122779816252 38.0 32.0 40.0 16.0 41.0 19 33.196594506165155 38.0 32.0 40.0 11.0 41.0 20 32.96916146968104 38.0 32.0 40.0 10.0 41.0 21 32.4415191042641 38.0 31.0 40.0 9.0 41.0 22 32.354505895248565 38.0 31.0 40.0 2.0 41.0 23 32.414700709109255 38.0 31.0 40.0 2.0 41.0 24 32.401393476914585 38.0 32.0 40.0 2.0 41.0 25 32.2459262580354 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8052288428750387 1101 2 0.8676452886322501 1101 3 0.8054517407272286 1101 4 0.929824846206337 1101 5 1.1370209128696231 1101 6 0.8932427823270999 1101 7 1.1090670584162368 1101 8 1.2317303579138539 1101 9 2.2835182054883525 1101 10 2.327062839467086 1101 11 1.4421388185404709 1101 12 1.5476046057042296 1101 13 1.050559439907687 1101 14 1.8578684843306732 1101 15 1.6386768710600847 1101 16 1.5212203829025057 1101 17 1.4198274155599293 1101 18 1.5829615195190812 1101 19 1.5243008004402867 1101 20 2.3629550493932427 1101 21 2.739521393334435 1101 22 2.625729834779822 1101 23 2.4580312734943064 1101 24 2.2885859662082524 1101 25 2.4066669788599384 1102 1 0.2826364281350102 1102 2 0.6426239329184504 1102 3 0.5438517875408841 1102 4 0.4310461637935532 1102 5 0.8636680778684536 1102 6 0.7338872302034716 1102 7 0.9853960594755762 1102 8 1.0040660818001612 1102 9 2.034560050622023 1102 10 2.0656209426936094 1102 11 1.218766497958633 1102 12 1.2292759738453682 1102 13 1.0694842822735637 1102 14 1.6960175110168692 1102 15 1.0914913007660019 1102 16 1.0404230305284017 1102 17 1.2111250828403186 1102 18 1.1380354320065038 1102 19 1.101849490261209 1102 20 1.745802369311491 1102 21 2.263856349862884 1102 22 2.1516915054964336 1102 23 2.1246344963972845 1102 24 1.9629822148418512 1102 25 2.0362831913456745 1103 1 0.4767653346302545 1103 2 0.5585907775712862 1103 3 0.6210737945327232 1103 4 0.5426434913978397 1103 5 0.7617423359233868 1103 6 0.6391640326388242 1103 7 0.8939775245156127 1103 8 0.8863194265058212 1103 9 1.7266258590554138 1103 10 1.9301945491874726 1103 11 1.1338052317710492 1103 12 1.1282328636251222 1103 13 1.0884962370027012 1103 14 1.7375001301851682 1103 15 1.2583221720509172 1103 16 1.1976815433153831 1103 17 1.0365993595769112 1103 18 0.7890620809307052 1103 19 0.8945607346280013 1103 20 1.5211096595984017 1103 21 2.2208419879563195 1103 22 2.007002225858063 1103 23 1.9333128916735802 1103 24 1.7355086945592362 1103 25 1.5636242556182296 1104 1 0.27412717612745396 1104 2 0.40025339568054363 1104 3 0.24065364511923093 1104 4 0.237089028623636 1104 5 0.3347140876571544 1104 6 0.37289440108519756 1104 7 0.550407384147185 1104 8 0.7510062893517571 1104 9 1.5793413153811535 1104 10 1.5429764571521112 1104 11 0.86927509746954 1104 12 0.788599263995458 1104 13 0.8600226734901142 1104 14 1.2573227362237986 1104 15 0.4065233881190693 1104 16 0.5655079538130323 1104 17 0.01589523447213992 1104 18 0.5873846747249161 1104 19 0.8480068271973806 1104 20 1.5627822312341735 1104 21 2.114158826874288 1104 22 1.9368121687990758 1104 23 1.6602355219640614 1104 24 1.509927499456687 1104 25 1.504614852695525 1105 1 -0.01767521457459864 1105 2 0.3652998957023357 1105 3 0.4167630804435767 1105 4 0.7329150706928189 1105 5 0.7499990260970932 1105 6 0.5385338014849381 1105 7 0.6916196416009299 1105 8 0.6896504546018036 1105 9 1.7007370065929983 1105 10 1.7556041675311 1105 11 1.088257410858482 1105 12 1.389766747822442 1105 13 0.8104254255352217 1105 14 1.1021626761403525 1105 15 1.252100902766493 1105 16 0.6635299703697726 1105 17 1.0874605531793549 1105 18 0.6727273604666522 1105 19 0.7450519735295629 1105 20 1.3726255725162702 1105 21 2.2333559968597996 1105 22 1.6937303602031442 1105 23 1.764792862032536 1105 24 1.6945180646710796 1105 25 1.4053366082605194 1106 1 0.19318932283432133 1106 2 0.2709684746861676 1106 3 -0.2124305214191331 1106 4 0.07934583962946817 1106 5 0.1636396181355977 1106 6 -0.07747029583444487 1106 7 0.10150855011106685 1106 8 0.1359387933913503 1106 9 0.9050304378514618 1106 10 0.9884248831432281 1106 11 0.44970547919677983 1106 12 0.561774670782377 1106 13 0.590636238252408 1106 14 1.0386371199659763 1106 15 -0.04202766471626518 1106 16 0.05494522091048992 1106 17 -0.09037085601374883 1106 18 -0.6088989493146357 1106 19 0.044892840164934 1106 20 0.6422004102258967 1106 21 -4.819194237312271 1106 22 -1.3057338287525049 1106 23 -0.3396847574168511 1106 24 0.20004647749814097 1106 25 0.5472888018407431 1107 1 0.35342790416281744 1107 2 -0.024939902895322774 1107 3 0.22721767351376343 1107 4 0.1585204382267591 1107 5 0.39794101269263393 1107 6 -0.011529222329912159 1107 7 0.2523583724567189 1107 8 0.28767131510794997 1107 9 1.2496217144343085 1107 10 1.6137805575297257 1107 11 -0.08551612379503126 1107 12 0.11374126716743405 1107 13 -0.029191289708734303 1107 14 0.7548228904829486 1107 15 0.39465933416159515 1107 16 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1109 3 -0.18865176147555474 1109 4 0.16924878502427276 1109 5 0.3883080947692932 1109 6 0.13145757435003702 1109 7 0.39410863475713853 1109 8 0.2503524582183658 1109 9 1.2440893398104471 1109 10 1.5963047705419022 1109 11 0.4129845220007198 1109 12 0.3448471534312034 1109 13 0.23982730851627565 1109 14 0.7279544977698791 1109 15 0.4805512075232983 1109 16 0.22331541090613882 1109 17 -0.46326630285254566 1109 18 0.22801352173140543 1109 19 0.29684245624666517 1109 20 1.0516510843834865 1109 21 0.9955122961258951 1109 22 1.0371382174347339 1109 23 0.6284642332339132 1109 24 0.4243974041520673 1109 25 0.6626032093988385 1110 1 0.3059084777781784 1110 2 0.4416394415149938 1110 3 0.19552126537151793 1110 4 0.40827762976267934 1110 5 0.5700867496795041 1110 6 0.29582975239683407 1110 7 0.2570110060918722 1110 8 0.374805574186972 1110 9 1.3839173342515814 1110 10 1.0452148281515399 1110 11 0.11290210371001308 1110 12 0.26592070364689846 1110 13 0.11199978313585035 1110 14 1.0162861137089578 1110 15 0.1322192422836892 1110 16 0.35816537385533564 1110 17 0.16583409027688134 1110 18 0.4251324577287363 1110 19 0.209212435363483 1110 20 1.2667041073716163 1110 21 1.0789976167713036 1110 22 1.510411654044585 1110 23 1.2090988457556762 1110 24 0.8880096872218886 1110 25 1.1835772921315026 1111 1 -0.10755692975734732 1111 2 0.07014677611220677 1111 3 -0.179754136583405 1111 4 -0.012557995838420766 1111 5 0.1290994518113422 1111 6 -0.41588706710967216 1111 7 0.017511406496630855 1111 8 0.011139601467267113 1111 9 1.0345178424423942 1111 10 0.9031108825485425 1111 11 -0.0633396457751374 1111 12 0.09392195829244798 1111 13 -0.18564607435699543 1111 14 0.07923953746648493 1111 15 -0.05517958460292505 1111 16 -0.07516327599564931 1111 17 -0.7139461950329462 1111 18 -0.34395405212702457 1111 19 -0.10240223805266879 1111 20 0.28838566223498674 1111 21 0.9776404623232509 1111 22 0.9262423850768151 1111 23 0.7149190620402095 1111 24 0.7188723187057064 1111 25 0.7679632546135124 1112 1 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2.717277045391299 2203 22 2.807043078413759 2203 23 2.5907820387093565 2203 24 2.2789546447711118 2203 25 2.3830730432257923 2204 1 0.8873999910614074 2204 2 1.0915229976105785 2204 3 1.0080864252053097 2204 4 1.0666760291746513 2204 5 1.3291374037154782 2204 6 1.307792910370523 2204 7 1.7213081049323051 2204 8 1.4446395713068156 2204 9 2.607605418346207 2204 10 2.8736780498787375 2204 11 1.9591009883986672 2204 12 2.116934384399336 2204 13 1.6933816028859354 2204 14 2.6030776059910323 2204 15 2.232526009155869 2204 16 2.2536836898627186 2204 17 1.9384797107860123 2204 18 1.9903569825776017 2204 19 2.142143746585603 2204 20 2.9225167395663902 2204 21 3.211410368222637 2204 22 3.0512926524995017 2204 23 3.1163810900291082 2204 24 2.837925682601636 2204 25 3.0996804903073993 2205 1 0.47386864868617096 2205 2 0.5638430289094813 2205 3 0.6089330171023697 2205 4 0.6109618055613666 2205 5 0.9285385948126716 2205 6 0.35439350157205496 2205 7 0.5410894473075487 2205 8 1.034346796137278 2205 9 1.8431351088348187 2205 10 1.8251484463315286 2205 11 1.0499710544741347 2205 12 1.2796234819475885 2205 13 1.2185239694308834 2205 14 1.4584016800651085 2205 15 0.30107930423498885 2205 16 0.3080329334725249 2205 17 0.3049159827387129 2205 18 0.8938411592425268 2205 19 0.9129662122437523 2205 20 1.7198237296550687 2205 21 1.5282628721349951 2205 22 1.2005710388670927 2205 23 0.27589139258518713 2205 24 0.3879169884614839 2205 25 0.5969961745362298 2206 1 -1.3760605240514039 2206 2 -3.5089080368721817 2206 3 -1.333151867685281 2206 4 -2.2269435961978488 2206 5 -0.538356742729821 2206 6 -1.0041536626392684 2206 7 -0.7838855945142633 2206 8 -0.15852933035346695 2206 9 0.9640682748808977 2206 10 0.5976323921520468 2206 11 0.29951978363067866 2206 12 0.10743566153854545 2206 13 -0.038421824104268865 2206 14 0.5271756264252616 2206 15 -0.21497824797482679 2206 16 0.18200425180396707 2206 17 0.16317106881070487 2206 18 -0.17996017365909722 2206 19 0.16877742048939126 2206 20 0.8432702516992094 2206 21 1.47292856745635 2206 22 1.1792034656114687 2206 23 0.8719925204250245 2206 24 0.8457747673185381 2206 25 0.5697688257693017 2207 1 0.3465040114841571 2207 2 0.4419888976898818 2207 3 0.2760998209034149 2207 4 0.2548381041494423 2207 5 0.5683309572346289 2207 6 0.3253470756910062 2207 7 0.5329575649924223 2207 8 0.5642361950915813 2207 9 0.8725293347950362 2207 10 1.6298358978535923 2207 11 0.2797732086628102 2207 12 0.4238153137029457 2207 13 0.6276761687311918 2207 14 1.6435501512960542 2207 15 0.8422884943479048 2207 16 -0.00404643320246123 2207 17 0.18829002202243572 2207 18 0.5825610191544897 2207 19 0.8405263745214953 2207 20 1.3466088034780412 2207 21 1.6325405338759964 2207 22 1.5760843619991185 2207 23 0.6785185397309874 2207 24 1.06162870097819 2207 25 0.8406457980682447 2208 1 0.15124906403711336 2208 2 0.46308784537649217 2208 3 0.5003113384340772 2208 4 0.41704782742604607 2208 5 0.47904706278141873 2208 6 0.4350939955601021 2208 7 0.8665757777059113 2208 8 0.9052012228159754 2208 9 1.7883188645919752 2208 10 1.820663847954389 2208 11 1.0698015837152184 2208 12 1.0008641326529286 2208 13 0.8204918462847317 2208 14 1.75842145072545 2208 15 1.2640162775975057 2208 16 1.1932266580978563 2208 17 1.0141299458371975 2208 18 0.6824426774064776 2208 19 0.9954015120616404 2208 20 1.942526295385555 2208 21 2.4827750380526084 2208 22 2.137481747881953 2208 23 2.118650597080645 2208 24 1.7182582690433534 2208 25 1.514565051618419 2209 1 0.44352306433191657 2209 2 0.3174845360721186 2209 3 0.3812788607374884 2209 4 0.31226098790063617 2209 5 0.5652097062941905 2209 6 0.33228702973643465 2209 7 0.5229471947124225 2209 8 0.39212614812031177 2209 9 1.2546883864227603 2209 10 1.228813103571479 2209 11 0.4524282036810092 2209 12 0.6693286425628244 2209 13 0.4147824539342224 2209 14 0.9710578273366366 2209 15 0.6249396259284623 2209 16 0.4614225677939032 2209 17 -0.00403800416760447 2209 18 0.23574835521256432 2209 19 0.06345282686904596 2209 20 0.8388026260445827 2209 21 1.6537386062239463 2209 22 1.3811596990332156 2209 23 1.1710641384621567 2209 24 0.685748752219574 2209 25 0.614691630639058 2210 1 -0.05290374810174825 2210 2 0.22724495931436905 2210 3 0.3046346464433505 2210 4 0.3665698317306223 2210 5 0.09534698717544643 2210 6 0.279924674803965 2210 7 0.4080012214564164 2210 8 0.3873323006715843 2210 9 1.6449947884705303 2210 10 1.6488357178045767 2210 11 0.7805450022640414 2210 12 0.7518557946724442 2210 13 0.6073747896052879 2210 14 1.3704441445694684 2210 15 0.7336151209327468 2210 16 0.8471473909612328 2210 17 0.6154278097251193 2210 18 0.6680423124952455 2210 19 0.9073008536731493 2210 20 1.7218551312992716 2210 21 1.8579072094366467 2210 22 1.6687900834983047 2210 23 1.878065070316545 2210 24 1.7745075360455473 2210 25 1.6525503541141546 2211 1 0.555084223342341 2211 2 0.3600173841382457 2211 3 0.4003485332770289 2211 4 0.42207404614377 2211 5 0.7456348370616794 2211 6 0.3284746452997851 2211 7 0.8501661296236662 2211 8 0.8388142943458092 2211 9 1.9021496702297362 2211 10 1.9492362413902136 2211 11 1.2385033441759887 2211 12 0.3919685404479267 2211 13 0.5135930115011789 2211 14 1.2971034532095445 2211 15 1.11847203269879 2211 16 1.0061321587807086 2211 17 0.6858053911844024 2211 18 0.5507234670786758 2211 19 0.5062373242584357 2211 20 1.3416764265187808 2211 21 2.215951823270302 2211 22 2.1875134094767397 2211 23 2.134261967097032 2211 24 1.9976893623466054 2211 25 1.7891754015511765 2212 1 0.2373054911307655 2212 2 0.22097056315364938 2212 3 0.08445363979818055 2212 4 0.11090026277154408 2212 5 0.19903527443263158 2212 6 -0.07263433335260316 2212 7 0.32519734060993244 2212 8 0.046504427736277165 2212 9 -9.604598944256097 2212 10 -2.072730085785018 2212 11 -0.8965438652564863 2212 12 -0.20459079246291623 2212 13 -0.07965817378211426 2212 14 0.75064054814667 2212 15 0.38105562491394807 2212 16 0.15435472606746004 2212 17 0.2480753551194823 2212 18 0.5092309595508269 2212 19 0.5818718642163461 2212 20 1.2491137016970555 2212 21 1.7190440552804773 2212 22 1.2780578071672757 2212 23 1.3208000455014357 2212 24 0.9952637322617832 2212 25 0.9614071514254476 2213 1 0.26371891425037575 2213 2 0.15152537629754192 2213 3 -0.07207128761375614 2213 4 0.2445129804614865 2213 5 0.14108935130213496 2213 6 -0.08711611219469972 2213 7 0.24835832753042197 2213 8 0.36393490731837375 2213 9 0.9130130401291048 2213 10 -9.15904761703461 2213 11 -3.0165662920279672 2213 12 -1.0459219781477955 2213 13 -0.12724814714636778 2213 14 0.27648498696858326 2213 15 0.19529808983153174 2213 16 0.3764393546616702 2213 17 0.11409875672697467 2213 18 0.15913436684419935 2213 19 0.5309386626561334 2213 20 1.3068751107825136 2213 21 1.11608779184035 2213 22 1.3965223573073864 2213 23 0.8970540381913494 2213 24 1.1163524601562287 2213 25 0.7181398353168618 2214 1 -2.0762240198896933 2214 2 -2.4611672520921566 2214 3 -2.3664158391478267 2214 4 -2.398198055175257 2214 5 -2.150529751053245 2214 6 -2.2465882643039556 2214 7 -10.734851706285966 2214 8 -4.135565596703053 2214 9 -3.1059525336803375 2214 10 -2.403735027586361 2214 11 -3.1178553650018976 2214 12 -11.907204647617224 2214 13 -5.428626937065033 2214 14 -13.473062185703157 2214 15 -7.127953872231661 2214 16 -5.084568280895201 2214 17 -4.021519775422355 2214 18 -3.704648045240546 2214 19 -3.9154229362288007 2214 20 -4.0297529349833106 2214 21 -13.23438415549252 2214 22 -15.853688924400425 2214 23 -10.218197729277712 2214 24 -8.550702640529263 2214 25 -16.262216905502996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43035.0 3 0.0 4 658.0 5 6854.0 6 8294.0 7 6818.0 8 5578.0 9 4687.0 10 4114.0 11 3816.0 12 3585.0 13 3577.0 14 3494.0 15 3460.0 16 3697.0 17 4128.0 18 4553.0 19 5591.0 20 7086.0 21 9306.0 22 11637.0 23 14229.0 24 17153.0 25 19875.0 26 22772.0 27 25512.0 28 28678.0 29 34044.0 30 40654.0 31 48273.0 32 59715.0 33 72945.0 34 88378.0 35 104244.0 36 127828.0 37 166683.0 38 318835.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45933938690353 19.065521347984294 12.869086294873064 24.606052970239112 2 17.427325842966233 21.42777874423376 36.62231633503896 24.522579077761048 3 19.918201482270113 25.640348937031636 29.115923719695747 25.3255258610025 4 13.509813418344471 16.801420917598474 35.024359230041405 34.66440643401565 5 13.835163286177432 38.65199305407683 32.696148851493405 14.81669480825233 6 31.87635939956619 35.48485532352304 18.15464297040942 14.484142306501353 7 29.74610467409119 32.4832918221773 20.046664437410126 17.723939066321382 8 28.258255848228448 33.524792858444705 19.198705135580497 19.01824615774635 9 27.943256030171476 15.646804777650313 19.181681175068043 37.228258017110164 10 16.20198687910028 30.45870665417057 30.18609184629803 23.153214620431115 11 35.42205260474462 22.881714302852174 21.728473742614643 19.96775934978856 12 24.775870743193646 26.720136280785205 27.64357056257062 20.86042241345053 13 29.313850597033053 20.992201007376128 25.884153244731817 23.809795150859 14 23.424511587908178 20.961776510184357 26.59396998412759 29.019741917779875 15 25.3740485715828 28.174876891610634 22.892687271702165 23.558387265104393 16 24.341009250387245 28.357407732298086 24.200356580010826 23.101226437303847 17 23.185073165391948 28.59999445177127 25.370850356023837 22.84408202681294 18 22.869490177143557 26.753872648876087 27.597491934558775 22.779145239421585 19 24.296032047720693 26.454739642259746 27.147517433783115 22.101710876236446 20 24.88759663291986 25.56741521709422 26.91685963679084 22.62812851319508 21 25.786764347496806 25.016365538263113 26.097578350248764 23.099291763991317 22 25.20667973301462 25.721260907576333 26.029349796731815 23.04270956267723 23 24.306645362477383 25.615338416446914 26.501746648601593 23.576269572474107 24 24.124244055536145 25.9124941331362 26.516799848848592 23.446461962479063 25 23.958391907504918 25.68184084469766 27.073450699103823 23.286316548693602 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 143.0 1 143.0 2 225.0 3 307.0 4 307.0 5 307.0 6 681.0 7 1055.0 8 1055.0 9 1055.0 10 1322.5 11 1590.0 12 1590.0 13 1590.0 14 2041.5 15 2493.0 16 2493.0 17 2493.0 18 5339.5 19 8186.0 20 8186.0 21 8186.0 22 15226.5 23 22267.0 24 22267.0 25 22267.0 26 34784.5 27 47302.0 28 47302.0 29 47302.0 30 61236.0 31 75170.0 32 75170.0 33 75170.0 34 91092.5 35 107015.0 36 107015.0 37 107015.0 38 119092.0 39 131169.0 40 131169.0 41 131169.0 42 144552.5 43 157936.0 44 157936.0 45 157936.0 46 171557.0 47 185178.0 48 185178.0 49 185178.0 50 182715.0 51 180252.0 52 180252.0 53 180252.0 54 163182.5 55 146113.0 56 146113.0 57 146113.0 58 128184.5 59 110256.0 60 110256.0 61 110256.0 62 92123.5 63 73991.0 64 73991.0 65 73991.0 66 57701.0 67 41411.0 68 41411.0 69 41411.0 70 31440.5 71 21470.0 72 21470.0 73 21470.0 74 15817.5 75 10165.0 76 10165.0 77 10165.0 78 8031.0 79 5897.0 80 5897.0 81 5897.0 82 4296.5 83 2696.0 84 2696.0 85 2696.0 86 1966.0 87 1236.0 88 1236.0 89 1236.0 90 847.0 91 458.0 92 458.0 93 458.0 94 242.5 95 27.0 96 27.0 97 27.0 98 15.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026840887518687406 2 0.01214587647493676 3 0.0015744654689732835 4 0.0 5 0.004048625491645586 6 0.0012745672844069438 7 5.997963691326794E-4 8 0.038911789447482574 9 0.005698065506760455 10 0.0026990836610970574 11 0.004948320045344606 12 0.00517324368376936 13 0.0017993891073980381 14 0.0018743636535396234 15 1.4994909228316985E-4 16 2.998981845663397E-4 17 0.002024312745822793 18 6.747709152742644E-4 19 0.0018743636535396234 20 0.6983878973088636 21 0.014320138313042723 22 0.005997963691326795 23 0.006672734606601059 24 0.0017993891073980381 25 5.248218229910945E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1333786.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.42360267298107 #Duplication Level Percentage of deduplicated Percentage of total 1 73.99521016571607 36.571098669340806 2 13.212855323748403 13.060538233930474 3 4.930861204172666 7.311027749719405 4 2.5062033856951618 4.954624014091104 5 1.424968958075043 3.5213549802616364 6 0.8778165342854648 2.6030913366178847 7 0.5926237364583093 2.0502720059705046 8 0.4072510861727507 1.6102252696913604 9 0.294419824880291 1.309615959953895 >10 1.5651911670662138 14.04282367143981 >50 0.12068498414584733 4.097260051661538 >100 0.06593395188620182 5.742606466599693 >500 0.0045634382429279 1.5078619782744556 >1k 0.0014162394547017623 1.617599612447411 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3811 0.2857279953455802 No Hit GTGGTATCAACGCAGAGTACATGGG 3202 0.24006849674535496 No Hit TATCAACGCAGAGTACTTTTTTTTT 3126 0.2343704312385945 No Hit GGTATCAACGCAGAGTACTTTTTTT 2930 0.21967542019484385 No Hit GGTATCAACGCAGAGTACATGGGAT 2071 0.1552722850592224 No Hit GGTATCAACGCAGAGTACATGGGAG 1682 0.12610718661014583 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1449 0.10863811735915656 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.4994909228316987E-4 0.0 2 0.0 0.0 0.0 1.4994909228316987E-4 0.0 3 0.0 0.0 0.0 1.4994909228316987E-4 0.0 4 0.0 0.0 0.0 1.4994909228316987E-4 0.0 5 0.0 0.0 0.0 1.4994909228316987E-4 0.0 6 0.0 0.0 0.0 1.4994909228316987E-4 7.497454614158494E-5 7 0.0 0.0 0.0 1.4994909228316987E-4 7.497454614158494E-5 8 0.0 0.0 0.0 1.4994909228316987E-4 7.497454614158494E-5 9 0.0 0.0 0.0 1.4994909228316987E-4 7.497454614158494E-5 10 0.0 0.0 0.0 1.4994909228316987E-4 7.497454614158494E-5 11 0.0 0.0 0.0 2.249236384247548E-4 7.497454614158494E-5 12 0.0 0.0 0.0 2.249236384247548E-4 7.497454614158494E-5 13 0.0 0.0 0.0 2.249236384247548E-4 7.497454614158494E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGGAT 65 3.4570476E-6 14.580029 10 ATAACGC 40 0.005349418 14.221394 3 AAGTTCG 115 2.3646862E-11 14.108947 14 GTTCGAC 95 4.5838533E-9 14.066328 16 TCGACCG 105 1.2769306E-9 13.635726 18 AGACCCG 70 7.412951E-6 13.544184 5 AGTTCGA 120 4.7293724E-11 13.522095 15 TAATACC 120 5.2750693E-11 13.431315 4 AACGCCG 85 2.7648457E-7 13.38484 5 CCGAGTA 85 2.778761E-7 13.379321 9 ACGCCGA 100 1.0353688E-8 13.2733 6 CCCCCGC 75 1.40861885E-5 12.726196 19 CCTCTAG 165 0.0 12.639816 1 TGCACGG 105 2.060915E-8 12.636025 12 ACGGCCC 85 3.7391546E-6 12.352365 15 GGTATCA 2555 0.0 12.244048 1 CGACCGT 110 3.5706762E-8 12.147734 19 CGGTTTG 55 0.0029629944 12.147275 14 TAACGCA 65 8.158474E-4 11.668835 4 TGTACCG 65 8.158474E-4 11.668835 5 >>END_MODULE