FastQCFastQC Report
Thu 26 May 2016
SRR1364244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199726
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN21120.1760401958447179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG655.4574208E-513.1520535
ACGGCCC1102.73576E-912.9571115
GCCCCCG1405.2750693E-1112.21670418
CGCGCCA550.00307471712.0872410
GTGCACG1103.828427E-812.08673611
ACATGGT5500.011.2309053
TGCGCGC1451.2114469E-911.14013719
CAACGGA600.00586019411.0869194
GCCTAAT600.005863842611.0859911
GGTATCA9150.010.9042541
GTCTGCG1053.455225E-610.85929317
GTATAAG1355.6596036E-810.5580871
CTTATCC1002.3885668E-510.453383
CGGCCCC1659.240466E-1010.36568816
TTGCGCG1106.0260354E-610.36568818
TCTGCGC1201.5182595E-610.29370418
GTGTTAT1502.431625E-810.1357631
GGACCGC750.002649806210.1319516
AGTAGTC750.00265072510.1315287
CCCCCGC1606.1409082E-910.09574919
Produced by FastQC (version 0.11.5)