FastQCFastQC Report
Thu 26 May 2016
SRR1364244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199726
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG16560.1380315171964265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGT508.828749E-515.1761498
CCATGCG400.005345257514.2228969
GGACCGA759.840078E-713.911476
AGACCCG551.9872434E-413.7953495
ATTCGTC500.00145799713.34926416
GTCGGTT655.5508557E-513.1282812
GCCCCCG905.164602E-712.71358618
ACGGCCC1051.8913852E-812.71358615
TTCGCGG603.9651606E-412.71358616
CGGTTTG603.9693702E-412.711987514
AGGACCG905.510901E-712.6457375
TTGACCG657.7760447E-411.73561718
ACAACCG752.1051448E-411.3811635
CGTTCGC600.00571483311.12438715
ATTGGCG600.00571483311.12438717
CGAGTGG600.005958159511.06225210
GGGTATC1553.2014214E-1011.0177031
GGCCTAT803.815577E-410.67341
GACTATC803.8244092E-410.670737
CTGGTCG909.695317E-510.5354799
Produced by FastQC (version 0.11.5)