FastQCFastQC Report
Thu 26 May 2016
SRR1364243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4583802
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN87940.1918494734283898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33950.012.6501671
GGACCGA2550.011.54707056
CGACCTA752.0736872E-411.4010718
AGACCCG2450.011.2428785
AGGACCG3150.010.8551925
TGACCGA4350.010.70238919
TTGACCG4750.010.60099518
AACTGGC7600.010.50098617
AACCGTC1106.0653474E-610.3617747
ACATGGT20400.010.2948863
GTATCAA74950.010.1418221
GTCCTAG7400.09.886821
GTGGTAT11950.09.70039751
CGCGGTT2106.730261E-119.50089218
ACAACCG2600.09.4982945
GTCCTAA2950.09.3405751
TACATGT23550.09.3202632
CATGGTA14400.09.0410214
TTCGCGG2252.6193447E-108.86778916
GTCCCAG17500.08.7414891
Produced by FastQC (version 0.11.5)