##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364243_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4583802 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.507880794152975 29.0 22.0 31.0 2.0 33.0 2 25.032222377842672 30.0 25.0 31.0 2.0 34.0 3 24.64860742239739 30.0 22.0 31.0 2.0 34.0 4 27.696740609651116 33.0 25.0 35.0 2.0 37.0 5 28.11888886125535 33.0 26.0 35.0 2.0 37.0 6 28.835860711261088 35.0 28.0 35.0 2.0 37.0 7 28.78941346070358 35.0 28.0 35.0 2.0 37.0 8 27.0119333252178 33.0 19.0 35.0 2.0 37.0 9 26.774321403934987 32.0 17.0 37.0 2.0 39.0 10 28.510626986069642 34.0 27.0 37.0 2.0 39.0 11 29.06266195616652 34.0 27.0 38.0 2.0 39.0 12 27.656435421948853 34.0 19.0 37.0 2.0 39.0 13 27.43793863696556 33.0 18.0 37.0 2.0 39.0 14 27.898379336629286 33.0 17.0 38.0 2.0 40.0 15 27.43782584849869 33.0 17.0 38.0 2.0 40.0 16 28.22285888439335 33.0 22.0 38.0 2.0 40.0 17 28.776620587014886 34.0 25.0 39.0 2.0 40.0 18 29.31845354576834 35.0 27.0 39.0 2.0 40.0 19 29.98141739106532 36.0 27.0 40.0 2.0 41.0 20 30.02736636530112 36.0 27.0 40.0 2.0 41.0 21 30.19161407931669 36.0 29.0 40.0 2.0 41.0 22 30.073124013646314 36.0 28.0 40.0 2.0 41.0 23 29.716002130982098 36.0 27.0 40.0 2.0 41.0 24 29.6597549370588 36.0 27.0 40.0 2.0 41.0 25 29.332804732839683 36.0 27.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.4680611656894378 1101 2 -0.49665079950254665 1101 3 -2.8690566814860716 1101 4 -3.132493133617313 1101 5 -3.8294697037943592 1101 6 -1.7962358846752409 1101 7 -1.257348123008473 1101 8 -5.2573180076108486 1101 9 -6.205945231495001 1101 10 -6.259705167567539 1101 11 -3.2917936812910504 1101 12 -0.4689435595038631 1101 13 0.030722194066125752 1101 14 -7.27984985496116 1101 15 -9.08622343523076 1101 16 -4.115153991899998 1101 17 -2.088757203793172 1101 18 -6.846947546383742 1101 19 -3.3395975130844207 1101 20 -2.1366380912241603 1101 21 -2.0856083209545737 1101 22 -2.0736974560816677 1101 23 -6.957438139909581 1101 24 -3.797595277205712 1101 25 -2.6730886508250293 1102 1 1.8057713154457709 1102 2 1.326469404244044 1102 3 1.7574947031661488 1102 4 1.8658134008564637 1102 5 1.3564261588038349 1102 6 0.7684258042545054 1102 7 0.6138689283312893 1102 8 2.1755928848722093 1102 9 -3.33783010161898 1102 10 -0.5479811791006028 1102 11 0.11767704385054856 1102 12 -4.029059971071813 1102 13 -6.162662446127509 1102 14 -6.1554856898839425 1102 15 -1.0811224878042474 1102 16 0.5374739055449211 1102 17 -4.2678557890206505 1102 18 -1.5031780034023043 1102 19 -0.6597114763856844 1102 20 -0.251633826011215 1102 21 -0.10541743316807839 1102 22 -0.11383430229937375 1102 23 0.13774292947628552 1102 24 0.19375770698074035 1102 25 0.4165813189073937 1103 1 -0.8514984492425981 1103 2 0.9840105004916317 1103 3 2.001493066314339 1103 4 2.745747659289375 1103 5 2.4425506243884705 1103 6 1.6574242173890958 1103 7 1.536136908370235 1103 8 3.1521334183000036 1103 9 4.246944271950159 1103 10 2.6963643598297473 1103 11 2.192324403339466 1103 12 3.4373151533200073 1103 13 3.7208503653856795 1103 14 -2.62376612004795 1103 15 1.761947411820369 1103 16 2.3382515992915174 1103 17 2.4585195055951097 1103 18 2.2319416545718624 1103 19 1.8807083066625516 1103 20 1.850243500889274 1103 21 1.6420773162339088 1103 22 1.7135686855901362 1103 23 2.171828571573151 1103 24 1.9504403206585863 1103 25 1.9486331226462141 1104 1 2.077862036608984 1104 2 1.5134137178571194 1104 3 2.0881864717811744 1104 4 2.504725466573994 1104 5 1.582323879465953 1104 6 0.9255362716338631 1104 7 0.7290960149949548 1104 8 2.2714974695209342 1104 9 -3.307964538271534 1104 10 -0.5122677642803843 1104 11 0.1813116124391172 1104 12 1.8220003705644423 1104 13 2.029178744060978 1104 14 1.781421431719636 1104 15 -3.348715357708709 1104 16 -4.259214755980452 1104 17 -0.9201361417944902 1104 18 -0.2366760945070574 1104 19 0.09315371676767015 1104 20 0.3609301277444885 1104 21 0.015351697864470992 1104 22 -0.04914989310414697 1104 23 -0.2482416135052361 1104 24 -6.5702365339536115 1104 25 -3.513174567019803 1105 1 -2.3532946088315008 1105 2 0.0670153871250605 1105 3 1.1583731949345655 1105 4 1.6398594054334268 1105 5 1.5631442113475345 1105 6 1.2661034208472834 1105 7 0.47600893879473105 1105 8 2.838786436343284 1105 9 3.179246794809515 1105 10 1.8093667790639678 1105 11 1.6800280517947925 1105 12 2.2669918254406767 1105 13 2.4392758661254774 1105 14 3.00339043896669 1105 15 3.531275104686628 1105 16 3.090813722967365 1105 17 1.8297619255954665 1105 18 1.944304085095279 1105 19 1.0275120811073357 1105 20 1.0940613279612066 1105 21 0.9789996920238764 1105 22 1.007372232166663 1105 23 1.5048249941259684 1105 24 1.6428874173381018 1105 25 1.753466084508279 1106 1 2.373358457253726 1106 2 1.593871214626251 1106 3 2.2636230896560257 1106 4 2.475672773461657 1106 5 1.6788424441529806 1106 6 1.1643525155112506 1106 7 1.0753601319613963 1106 8 2.6167409768032073 1106 9 3.6800745009134133 1106 10 1.728400723002924 1106 11 0.8947084534747347 1106 12 2.0063116518394857 1106 13 -4.181695588987257 1106 14 0.3010078327036716 1106 15 2.4193875688934092 1106 16 1.9008933337367218 1106 17 1.5942581715023714 1106 18 1.361493590621734 1106 19 1.0625501595676141 1106 20 0.7376576754938284 1106 21 0.24567015505343903 1106 22 0.38973871551899464 1106 23 0.7877260073539425 1106 24 0.7806885154422574 1106 25 0.8122583718827556 1107 1 2.1253391363935457 1107 2 1.4358976674028447 1107 3 2.192350477913042 1107 4 2.1566515108143953 1107 5 1.7856074884006077 1107 6 0.8577150656773149 1107 7 0.42814967811514393 1107 8 2.175656359794761 1107 9 3.535364583895653 1107 10 1.929922203348827 1107 11 0.8097432180398627 1107 12 2.488294197919231 1107 13 2.8250690253131268 1107 14 -3.3199385823053866 1107 15 1.2639183432453507 1107 16 1.4922442394781186 1107 17 1.1576828684228708 1107 18 1.367427316737107 1107 19 0.9417887732626831 1107 20 1.032557141717355 1107 21 0.5513098547831312 1107 22 0.3723925243005013 1107 23 1.009009528251255 1107 24 1.2069470679104164 1107 25 1.2143372818562845 1108 1 1.718270558114714 1108 2 1.049419970469387 1108 3 1.3553523473614284 1108 4 1.823834686120879 1108 5 1.1049156477129287 1108 6 0.39666762210880435 1108 7 0.0030895669659685154 1108 8 -4.624241133250749 1108 9 -5.670621861148639 1108 10 -2.361356889026979 1108 11 -0.789670104234407 1108 12 0.3437387488929531 1108 13 1.7584382029301118 1108 14 2.1237280908920155 1108 15 -3.80988951889751 1108 16 -0.3930528345324902 1108 17 0.2507034747081818 1108 18 0.262822160277139 1108 19 -0.010949525406424243 1108 20 -0.02940347147401212 1108 21 -0.37246599393650826 1108 22 -0.30957425330033317 1108 23 0.036942014524434796 1108 24 0.02428597463456228 1108 25 0.39959424124445064 1109 1 0.6290616065050081 1109 2 0.547568181687776 1109 3 0.662400031412048 1109 4 -1.5841792836525599 1109 5 -2.92352355068353 1109 6 -1.2553409774639057 1109 7 -0.971243500212104 1109 8 1.3376978116926175 1109 9 -4.0773649537269385 1109 10 -1.2028818461803716 1109 11 -0.5440349212663307 1109 12 -7.108227377336409 1109 13 -1.9090428377785074 1109 14 0.05962867967920715 1109 15 1.535136704226428 1109 16 1.1271413415365217 1109 17 0.6387584522262841 1109 18 0.3846324139856243 1109 19 -0.22760457706565873 1109 20 -0.18554354133380357 1109 21 -0.25247335406891125 1109 22 -0.39251258303236014 1109 23 -0.20458624701263517 1109 24 -0.28162344440814024 1109 25 -0.034249431109650175 1110 1 0.08833713476072091 1110 2 -0.07570464257250364 1110 3 -3.4514443277304387 1110 4 -3.052119408599463 1110 5 -0.8931781418375948 1110 6 -0.9277815085868326 1110 7 -1.131639447807359 1110 8 -5.062122735593899 1110 9 -8.27838149876284 1110 10 -3.545314720513119 1110 11 -1.6201022057068073 1110 12 0.6872890259294344 1110 13 1.1207010523432963 1110 14 1.2586041889035897 1110 15 1.909518009098992 1110 16 1.4511898127542935 1110 17 0.934430290672406 1110 18 0.4428888877107475 1110 19 -0.4044214121031757 1110 20 -0.46545053743393794 1110 21 -0.5411611227545414 1110 22 -0.8364615810994671 1110 23 -0.41262790188251586 1110 24 -0.20309141594647784 1110 25 0.01055209773889132 1111 1 1.0373699476624587 1111 2 0.9445623113511239 1111 3 1.0726884488208803 1111 4 1.9039226958237592 1111 5 -1.8154033932613203 1111 6 -0.5558027602824467 1111 7 -0.8642578895861064 1111 8 1.597142424451377 1111 9 3.011873072376112 1111 10 1.5510647778511135 1111 11 1.227175546950761 1111 12 2.4503595723382787 1111 13 2.667582378572046 1111 14 2.847299607864322 1111 15 3.1759894178697863 1111 16 2.3346510079442204 1111 17 1.6942396578500478 1111 18 1.0846559613247777 1111 19 0.650463611229064 1111 20 0.6540966043297374 1111 21 0.44687963404343023 1111 22 0.3765829141764563 1111 23 0.7118713932740128 1111 24 0.6653393837922721 1111 25 0.649458629879458 1112 1 2.210228643481056 1112 2 1.5100027290321378 1112 3 2.044354426497609 1112 4 2.289519208003572 1112 5 1.7384101660607136 1112 6 0.9275762760502886 1112 7 0.6924903841113199 1112 8 -4.102441578733288 1112 9 1.311226799811692 1112 10 0.6088048908454624 1112 11 0.9362898886591502 1112 12 1.79204545416885 1112 13 2.25470936707357 1112 14 2.5672614071998296 1112 15 3.3432833716002825 1112 16 2.169478454241279 1112 17 1.5978748453318659 1112 18 1.2675039395220722 1112 19 0.9543355815871877 1112 20 0.8822345707376513 1112 21 0.7056854613672421 1112 22 0.5173052052976708 1112 23 0.9915615009095511 1112 24 1.0868567693051112 1112 25 1.03776233664534 1113 1 -0.264347125508273 1113 2 -0.16966858295781861 1113 3 -6.532228624349848 1113 4 -4.052117954582744 1113 5 -1.4818700599279886 1113 6 -1.4618369066239119 1113 7 -0.686924556252773 1113 8 1.1086286033131643 1113 9 -6.087941561770229 1113 10 -2.401601888815552 1113 11 -0.9535155099598072 1113 12 -5.018508720344649 1113 13 -0.5199997352490939 1113 14 0.6398085509537523 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2204 9 -2.29547537275624 2204 10 0.7355274185880631 2204 11 2.0679174480749936 2204 12 3.8279296738325392 2204 13 4.096894567036816 2204 14 4.3780767643052805 2204 15 4.774622116274561 2204 16 4.34744911785981 2204 17 3.375106991344264 2204 18 3.25343586662639 2204 19 2.6093183265930087 2204 20 2.478102881495886 2204 21 2.270905355723368 2204 22 2.0854466583410236 2204 23 2.607167263325856 2204 24 2.5313058399256185 2204 25 2.483372191942408 2205 1 -3.1723635779504313 2205 2 -3.774416763997003 2205 3 -0.3800691478366467 2205 4 -2.204439213443848 2205 5 -2.789024566432623 2205 6 -1.002803113681633 2205 7 -0.6721803013645591 2205 8 -4.646938367162804 2205 9 0.9284233833103954 2205 10 0.6231470400619088 2205 11 0.46318153763698433 2205 12 2.1804953460259995 2205 13 -4.417787037578297 2205 14 -0.16594890016411057 2205 15 2.249987274674716 2205 16 1.9114136148902219 2205 17 1.8389371025459234 2205 18 0.46178447892918584 2205 19 0.677636987730299 2205 20 0.5825385414132818 2205 21 0.5441040539272635 2205 22 0.6165796366839942 2205 23 0.835623238644768 2205 24 0.5546627098486248 2205 25 1.1780214921848753 2206 1 -7.427499394758655 2206 2 -5.741050802913623 2206 3 -2.445775998138732 2206 4 -0.9506020613310007 2206 5 -0.5950438828074702 2206 6 -1.1439223732225834 2206 7 -1.523564365873714 2206 8 -2.7291546365304526 2206 9 -5.665804692825812 2206 10 -2.4502304796705587 2206 11 -1.660041092145768 2206 12 -7.429796022741385 2206 13 -6.393600007219177 2206 14 -7.505261243368871 2206 15 -2.341463847636863 2206 16 -6.219068535345045 2206 17 -2.8369424791880498 2206 18 -1.9838730370707651 2206 19 -2.0022623498908203 2206 20 -2.058822700156526 2206 21 -1.9537392015431898 2206 22 -2.0415609521933646 2206 23 -1.51262083940966 2206 24 -1.4530427880712615 2206 25 -1.733706878012235 2207 1 -6.872473982117334 2207 2 -5.21551936919478 2207 3 -1.16496958651706 2207 4 0.005146252174224486 2207 5 0.6050193064117302 2207 6 0.15725809571144822 2207 7 0.12247728089498011 2207 8 1.6763348465017067 2207 9 -1.6812332946053523 2207 10 -0.47415241037868583 2207 11 -0.2598661249372327 2207 12 -4.272645292040117 2207 13 -4.896419227698104 2207 14 -1.0704940490773858 2207 15 -4.736623334320324 2207 16 -0.9280055464354291 2207 17 -0.1254118043114687 2207 18 0.04825229766179717 2207 19 -0.1425943398081735 2207 20 -0.12532804337306658 2207 21 -0.3318658234018024 2207 22 -0.2911183537516848 2207 23 -0.45119236767653703 2207 24 -0.5696504773394082 2207 25 -0.032102186242482844 2208 1 -3.976996267290545 2208 2 -4.454302707889237 2208 3 -1.4867753443122282 2208 4 -0.18175475997501422 2208 5 -3.071546116045237 2208 6 -1.361799474223254 2208 7 -0.8046999304984013 2208 8 -5.5375374285075125 2208 9 -8.445524632543801 2208 10 -4.040146132094808 2208 11 -1.6651324078358165 2208 12 -0.1668631054845484 2208 13 0.8635107906541926 2208 14 0.8555512580624622 2208 15 1.1777114161149278 2208 16 -4.68599103526201 2208 17 -7.169692688327402 2208 18 -3.095519008423981 2208 19 -2.6394729615201555 2208 20 -1.599082070121007 2208 21 -1.4720431062890746 2208 22 -1.163314346293916 2208 23 -0.881928678984881 2208 24 -0.8612761759560819 2208 25 -0.6441832756907857 2209 1 0.7542962994239133 2209 2 0.6972311143335297 2209 3 -2.41487865383446 2209 4 0.143355253735848 2209 5 0.37169119336446244 2209 6 0.03080203664770309 2209 7 0.024042617549170586 2209 8 1.6758309778070135 2209 9 -4.008580127638847 2209 10 -0.9716454474113014 2209 11 -0.14669316455744053 2209 12 -4.534918863050155 2209 13 -0.0479030100063369 2209 14 1.2196792747026244 2209 15 2.1180402522123067 2209 16 -3.1859431860328655 2209 17 -0.35511017056391125 2209 18 0.20860364843698065 2209 19 0.005592840113052944 2209 20 0.15488168598053065 2209 21 0.18489652800527168 2209 22 0.13416271678778457 2209 23 0.5207633760492456 2209 24 0.07940683594810238 2209 25 0.22166727160262312 2210 1 -0.664749909731011 2210 2 -3.464621096492234 2210 3 -1.3007771162291988 2210 4 -3.6717405377227266 2210 5 -3.779266502310044 2210 6 -1.9992188091761136 2210 7 -1.1541650821615121 2210 8 -5.350269153840742 2210 9 -4.862144810030092 2210 10 -2.2805825539354316 2210 11 -1.2806539477014915 2210 12 0.37703095482936533 2210 13 -7.335234383354276 2210 14 -2.05609833226357 2210 15 -5.534828306084716 2210 16 -1.877269935975196 2210 17 -1.2097173904211331 2210 18 -0.7247266429421018 2210 19 -1.0774787802598134 2210 20 -0.9627148381466348 2210 21 -1.2816108305595897 2210 22 -1.02527238546703 2210 23 -0.6322404283546454 2210 24 -0.7229151009567509 2210 25 -0.5929945419840479 2211 1 0.12970159184786212 2211 2 0.021903622573343995 2211 3 -3.4175313283017807 2211 4 -3.2212824257328876 2211 5 -0.998584581151313 2211 6 -1.1553074867024868 2211 7 -1.2562846979983995 2211 8 -5.252070233448258 2211 9 -0.08770749659805688 2211 10 -0.29150050987710685 2211 11 -6.23232145711334 2211 12 -0.8029970032196196 2211 13 0.37501430064952856 2211 14 1.0036380556144096 2211 15 -4.440021055223159 2211 16 -1.2043214395916735 2211 17 -6.415158646613701 2211 18 -2.4433268691159604 2211 19 -1.546695970974568 2211 20 -1.0905271985641711 2211 21 -1.0204783901608003 2211 22 -0.8135578074410681 2211 23 -0.5763968261310843 2211 24 -0.5999351826002588 2211 25 -0.3498253893531533 2212 1 1.952233758104505 2212 2 1.137390218494474 2212 3 2.0199717917558893 2212 4 2.4538155500882475 2212 5 -1.2956536686254125 2212 6 0.09041794590331875 2212 7 0.7928342655968592 2212 8 -1.5937638741477755 2212 9 2.5305450861308856 2212 10 1.9203047729557525 2212 11 1.5826432629362088 2212 12 3.113235448055793 2212 13 3.166338762690774 2212 14 3.487745906642573 2212 15 4.032143066555946 2212 16 3.151919978216835 2212 17 2.4649462167391576 2212 18 2.2386880677332215 2212 19 1.6077239101446246 2212 20 1.503592068657774 2212 21 1.161530182831381 2212 22 1.24794673506592 2212 23 1.32248298513219 2212 24 1.4778507789404927 2212 25 1.8333509796828977 2213 1 2.312298691737542 2213 2 1.8838186757294402 2213 3 2.385808956087729 2213 4 2.6358061123413243 2213 5 1.6159029130449873 2213 6 -0.47566213184913764 2213 7 0.7490947487665345 2213 8 -6.2350614867769245 2213 9 0.5247261344974667 2213 10 0.629533246319582 2213 11 0.8522309507866552 2213 12 2.5365932101572817 2213 13 -2.2169014083507648 2213 14 0.9605725149177182 2213 15 -3.839017331039404 2213 16 -0.08777922716894437 2213 17 0.947742812572713 2213 18 1.2140003754252895 2213 19 1.0834142971065823 2213 20 1.2655289425913452 2213 21 0.9876293907687987 2213 22 1.084965844144751 2213 23 1.3955892574796813 2213 24 1.5323995275597326 2213 25 1.7843354230489332 2214 1 1.620259315449612 2214 2 0.7371600435216763 2214 3 0.2227949924957322 2214 4 0.996455676025569 2214 5 0.9726849634686552 2214 6 -0.4443881865578483 2214 7 -0.18663608942095067 2214 8 1.5333247772740783 2214 9 2.357566613291702 2214 10 0.9610234896595351 2214 11 0.8608968214467438 2214 12 1.856194500134297 2214 13 0.19529330080926144 2214 14 1.7258268278598905 2214 15 2.8375770998902894 2214 16 1.2104117176983031 2214 17 0.4769263106868493 2214 18 0.5385934449605543 2214 19 0.2582344516955217 2214 20 -0.2721521117082055 2214 21 -0.3423674103262684 2214 22 -0.4816160143446204 2214 23 -6.978827686301425 2214 24 -3.4503691618563543 2214 25 -1.770576011920305 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 474824.0 3 12.0 4 3834.0 5 21452.0 6 28134.0 7 26163.0 8 22084.0 9 18951.0 10 16832.0 11 15512.0 12 14860.0 13 13863.0 14 13381.0 15 13318.0 16 13494.0 17 15007.0 18 18206.0 19 24980.0 20 36090.0 21 50515.0 22 66286.0 23 81965.0 24 96892.0 25 112300.0 26 127122.0 27 139786.0 28 151374.0 29 173933.0 30 185934.0 31 202327.0 32 248581.0 33 300190.0 34 348239.0 35 386003.0 36 408928.0 37 382671.0 38 329759.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.364556995234956 17.508316143539425 14.581121069713495 23.546005791512123 2 15.506958641725612 20.13587116946725 39.72959144628235 24.62757874252479 3 16.984198345488227 24.799964663631005 31.169257708838817 27.046579282041954 4 10.404033132742992 15.708777218690152 37.73992455270863 36.14726509585823 5 11.731946196625186 38.26362551507553 35.22997467893816 14.77445360936112 6 32.46473637505225 35.59220600995805 17.9455743595655 13.9974832554242 7 29.812888967050505 31.79675763453787 20.346722593052423 18.043630805359204 8 25.636177119059283 34.095090816826485 22.19207146356828 18.076660600545953 9 25.95808853478746 15.648702839320425 22.68927599713526 35.70393262875685 10 15.447746486359618 29.45293888487019 31.984435491262193 23.114879137508005 11 34.799338901978835 23.43376377032356 22.460205941307866 19.306691386389748 12 22.80817454677174 25.45228769235447 30.866178314733045 20.87335944614075 13 27.624062258777094 21.25738528972559 27.39861283090943 23.719939620587883 14 21.593390633639668 20.898105310310555 28.525967992552825 28.98253606349695 15 22.76888545050776 27.969218177312417 25.953521923726118 23.3083744484537 16 23.545175549207126 28.32729172868489 25.384735347969524 22.742797374138462 17 22.432131778893453 28.303079031553217 26.56662631982086 22.69816286973247 18 22.19316820004099 27.644985950174792 27.680572570567918 22.4812732792163 19 23.649904609348873 27.010984305104856 26.96588616854149 22.373224917004777 20 24.027054579382074 26.54247578153018 26.987535909503563 22.44293372958418 21 24.753856225132726 26.121544007980713 26.35518237769145 22.769417389195112 22 24.35215651089405 26.159654883014692 26.383828689128958 23.104359916962295 23 23.427820602586287 26.352098452072926 26.95609339752789 23.263987547812896 24 23.437294590971224 26.5763437482706 26.706208736367948 23.28015292439023 25 23.184965964967542 26.354883020980978 27.280872136770085 23.179278877281398 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10235.0 1 10235.0 2 6719.0 3 3203.0 4 3203.0 5 3203.0 6 4516.5 7 5830.0 8 5830.0 9 5830.0 10 8093.5 11 10357.0 12 10357.0 13 10357.0 14 16275.5 15 22194.0 16 22194.0 17 22194.0 18 37085.0 19 51976.0 20 51976.0 21 51976.0 22 79293.0 23 106610.0 24 106610.0 25 106610.0 26 149813.5 27 193017.0 28 193017.0 29 193017.0 30 240783.0 31 288549.0 32 288549.0 33 288549.0 34 339003.0 35 389457.0 36 389457.0 37 389457.0 38 436006.5 39 482556.0 40 482556.0 41 482556.0 42 519966.0 43 557376.0 44 557376.0 45 557376.0 46 577338.5 47 597301.0 48 597301.0 49 597301.0 50 581278.0 51 565255.0 52 565255.0 53 565255.0 54 520062.0 55 474869.0 56 474869.0 57 474869.0 58 414348.5 59 353828.0 60 353828.0 61 353828.0 62 291532.0 63 229236.0 64 229236.0 65 229236.0 66 178336.0 67 127436.0 68 127436.0 69 127436.0 70 95788.0 71 64140.0 72 64140.0 73 64140.0 74 46324.0 75 28508.0 76 28508.0 77 28508.0 78 21117.5 79 13727.0 80 13727.0 81 13727.0 82 9498.5 83 5270.0 84 5270.0 85 5270.0 86 3693.5 87 2117.0 88 2117.0 89 2117.0 90 1363.0 91 609.0 92 609.0 93 609.0 94 358.5 95 108.0 96 108.0 97 108.0 98 73.0 99 38.0 100 38.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21525798889219036 2 0.22583872514563239 3 0.23146724051344275 4 0.2936208850207753 5 0.23842652889457266 6 0.26229317933017177 7 0.26349305663726313 8 0.26096240631685225 9 0.269557891025834 10 0.26438751062982213 11 0.26209683577082954 12 0.2639511916090616 13 0.2627731302530083 14 0.2588680750172019 15 0.25923894618484833 16 0.2594571056952285 17 0.25699190322793175 18 0.2587153633599357 19 0.2519960504402241 20 0.2974168605013916 21 0.25697008727689374 22 0.2596752652056088 23 0.26342760878414906 24 0.26464930204227843 25 0.2627076823998942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4583802.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.22243149135144 #Duplication Level Percentage of deduplicated Percentage of total 1 94.99355964349428 80.00588569193805 2 2.98104320195231 5.021414136983748 3 0.8461246271354259 2.1378802032617608 4 0.36748689572731114 1.238025595974515 5 0.2006483901244896 0.8449547645554892 6 0.1318446877471282 0.6662568108768678 7 0.09062670410694346 0.5342960964553817 8 0.06170302487478442 0.4157423028260549 9 0.04461256758597044 0.3381641025446413 >10 0.24312009095253895 3.7431481248975653 >50 0.021787210698944095 1.2696845745200636 >100 0.01616714839144476 2.563597765135512 >500 8.675495608315201E-4 0.46713519620515154 >1k 3.827415445895743E-4 0.5648290908817409 >5k 2.5516102972393522E-5 0.18898554294357295 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 8794 0.1918494734283898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.726380415209907E-5 0.0 0.0 0.0 0.0 3 8.726380415209907E-5 0.0 0.0 0.0 0.0 4 8.726380415209907E-5 0.0 0.0 0.0 0.0 5 8.726380415209907E-5 0.0 0.0 0.0 0.0 6 1.0907975519012383E-4 0.0 0.0 0.0 0.0 7 1.0907975519012383E-4 0.0 0.0 0.0 0.0 8 1.0907975519012383E-4 0.0 0.0 0.0 0.0 9 1.0907975519012383E-4 0.0 0.0 0.0 0.0 10 1.0907975519012383E-4 0.0 0.0 0.0 0.0 11 1.0907975519012383E-4 0.0 0.0 6.54478531140743E-5 0.0 12 2.1815951038024766E-4 0.0 0.0 8.726380415209907E-5 2.1815951038024768E-5 13 2.617914124562972E-4 0.0 0.0 1.0907975519012383E-4 2.1815951038024768E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3395 0.0 12.650167 1 GGACCGA 255 0.0 11.5470705 6 CGACCTA 75 2.0736872E-4 11.40107 18 AGACCCG 245 0.0 11.242878 5 AGGACCG 315 0.0 10.855192 5 TGACCGA 435 0.0 10.702389 19 TTGACCG 475 0.0 10.600995 18 AACTGGC 760 0.0 10.500986 17 AACCGTC 110 6.0653474E-6 10.361774 7 ACATGGT 2040 0.0 10.294886 3 GTATCAA 7495 0.0 10.141822 1 GTCCTAG 740 0.0 9.88682 1 GTGGTAT 1195 0.0 9.7003975 1 CGCGGTT 210 6.730261E-11 9.500892 18 ACAACCG 260 0.0 9.498294 5 GTCCTAA 295 0.0 9.340575 1 TACATGT 2355 0.0 9.320263 2 CATGGTA 1440 0.0 9.041021 4 TTCGCGG 225 2.6193447E-10 8.867789 16 GTCCCAG 1750 0.0 8.741489 1 >>END_MODULE