FastQCFastQC Report
Thu 26 May 2016
SRR1364243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4583802
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT135550.29571521632042574No Hit
TATCAACGCAGAGTACTTTTTTTTT101340.22108284781934298No Hit
GGTATCAACGCAGAGTACTTTTTTT100360.21894488461761658No Hit
GTGGTATCAACGCAGAGTACATGGG68840.1501810069457625No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51870.11315933803423446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGA400.00540397214.20070813
GTGGTAT18900.013.2275471
GGTATCA52650.012.6622681
TGGTATC17750.012.5936132
TATATCG802.9595187E-511.8390895
ACCGGTC855.492932E-511.1430378
GGACCGA2700.010.5239796
AGGACCG3700.010.4951935
TACACCG2151.8189894E-1210.1320575
TTGACCG5250.010.02181118
GTAGGAC2850.09.9695423
CTTACAC7650.09.7805773
ATGCGTA2350.09.66856713
GACAACG1201.7635597E-59.4715817
GACCGGG1201.7635597E-59.4715817
ACCGTCC1701.7511411E-89.4715818
GTACTAG2002.8194336E-109.4697211
TGACCGA4700.09.36263219
AATGCGT2650.09.28881612
GTATCAA101250.09.2218711
Produced by FastQC (version 0.11.5)