FastQCFastQC Report
Thu 26 May 2016
SRR1364242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4668859
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN99550.21322126026937202No Hit
GTATCAACGCAGAGTACTTTTTTTT55260.11835868249608739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.012.4235181
GTATCAA81750.011.1351521
CGCGGTT1659.276846E-1010.36556418
TACATGT23200.010.1570622
GTGGTAT11050.010.1470111
GTACCGT1151.03039765E-59.9107356
ACATGGT18600.09.8606323
ACGGCCC2700.09.85400215
TTGCGCG4250.09.83712418
TATTCCG1554.085632E-89.8040635
TATCACG1951.6370905E-109.7454012
GCCCCCG4100.09.73351818
ACCGTCG900.00111654979.4979928
TTCGCGG1951.6789272E-99.258430516
CAACGGA3800.09.2529144
CTTACAC7200.09.239023
GTATTAC4350.09.1744161
TGGTATC11450.09.1283362
TGCGCGC4600.09.08845119
CTGGTCG3350.09.0727089
Produced by FastQC (version 0.11.5)