FastQCFastQC Report
Thu 26 May 2016
SRR1364242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4668859
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT172880.37028318910466135No Hit
TATCAACGCAGAGTACTTTTTTTTT141290.30262211816634427No Hit
GGTATCAACGCAGAGTACTTTTTTT116310.24911868188780167No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57700.12358479876989217No Hit
GTGGTATCAACGCAGAGTACATGGG53510.11461044336528475No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52980.11347526237138453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54000.013.51761
ACCGGTT550.003115670312.0671058
GGACCGA1850.011.2750566
GTGGTAT16450.011.17991351
CGGTTTG2700.010.95778814
TGGTATC15800.010.6223642
TATCACG1951.6370905E-1110.2115032
AGGACCG2800.010.1585325
ATCACGC2350.010.0863793
ACGGCCC3250.09.98533715
CGCGCCA2107.2759576E-129.9283210
CTTCGCG1451.512235E-79.87393915
ACCGCGT1252.6539492E-69.85611310
GTATCAA111600.09.8536341
CGCAATT1651.0657459E-89.76400611
TTGCGCG2650.09.72489818
TAGACAG7250.09.5466865
GACCGTT800.0045923479.48129757
ACGATAC3100.09.4811963
AGACGAA2500.09.47703310
Produced by FastQC (version 0.11.5)