FastQCFastQC Report
Thu 26 May 2016
SRR1364241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615297
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN55760.21320714243927172No Hit
GTATCAACGCAGAGTACTTTTTTTT30960.11838043633285245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.013.626591
GTACTAG1354.7257345E-911.2603171
TTACGCT700.0014893910.8594134
TTACGCC1053.4598743E-610.8594124
GTATCAA46050.010.6046881
CAACGGA2053.6379788E-1210.1972544
ACATGGT10300.09.87099553
ACCGAGT1356.4674896E-79.84956558
AGACCCG1752.584784E-99.7695355
TACATGT12100.09.7366382
TCAACGG2054.1836756E-119.7337433
GTCGAAA900.00111113779.50271516
TTGACCG2401.8189894E-129.50235118
GTATTAG3200.09.5008931
GTACTAA1002.7538463E-49.5008931
GCGTTGG1106.832437E-59.5008921
CTGGCTA4750.09.40196519
AACTGGC4150.09.38786417
GCCCTAG2252.7284841E-119.2897621
GTATTAC1752.667366E-89.2294381
Produced by FastQC (version 0.11.5)