FastQCFastQC Report
Thu 26 May 2016
SRR1364241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615297
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96560.3692123686143486No Hit
TATCAACGCAGAGTACTTTTTTTTT78450.2999659312116367No Hit
GGTATCAACGCAGAGTACTTTTTTT71900.2749209745585301No Hit
GTGGTATCAACGCAGAGTACATGGG35330.13508981962660455No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35250.13478392702626127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG601.4972211E-615.8031595
CGAAATC1450.013.07247513
GGTATCA31250.012.8638041
CGCGCCA1700.012.26576110
GGACCGA1650.012.0680976
CGCGGTC907.6651595E-611.58366513
GACGAAA1657.2759576E-1211.48837211
TGGTATC10500.011.289052
GTGGTAT11850.011.2011681
ACCGAGT1950.011.1839888
GACCGAC600.005960663311.0624237
ACGAAAT1553.255991E-1011.00660112
AGGACCG2950.010.9282875
AGACGAA1408.6674845E-910.832110
TTTGCGC1951.8189894E-1210.76651417
TCCCGAT1355.3269105E-810.60338517
GTCATAT2600.010.5749481
ACGGCCC2450.010.51682715
CGGTTTG2001.8189894E-1210.49613814
GTCGGTT1909.094947E-1210.47558112
Produced by FastQC (version 0.11.5)