FastQCFastQC Report
Thu 26 May 2016
SRR1364240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618016
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN51570.1969812254776136No Hit
GTATCAACGCAGAGTACTTTTTTTT35320.13491132216151466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGT1205.0931703E-1113.46153617
TTGCGCG4000.012.35246818
GGTATCA25100.012.3025461
ACATGGT13350.011.8869513
TAGCGCT907.4445416E-611.6140984
CTTCGCG1604.5474735E-1111.28393515
CGCGGTT1701.0913936E-1111.17870218
TTGACCG3150.011.16095918
CTACTCG700.001494514910.8549429
TTTGCGC4600.010.74168717
TGCGCGC4600.010.74106919
AGGACCG1605.475158E-1010.6845155
GTATCAA58050.010.6716291
CATGGTA9900.010.6542554
TAGATAG2500.010.6370295
TGACCGA3050.010.59207519
TACATGT15500.010.4819352
TTCGCGG1856.002665E-1110.27291116
ATTTGCG4450.010.24982716
CGCGCCA1502.4552719E-810.13108510
Produced by FastQC (version 0.11.5)