FastQCFastQC Report
Thu 26 May 2016
SRR1364240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618016
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT125720.48021096891692033No Hit
TATCAACGCAGAGTACTTTTTTTTT96710.36940186767384153No Hit
GGTATCAACGCAGAGTACTTTTTTT89720.34270226003202425No Hit
GTGGTATCAACGCAGAGTACATGGG50950.19461301993570704No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44440.16974686174568834No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29240.1116876291053989No Hit
GGTATCAACGCAGAGTACATGGGAT27140.10366628775377996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTTA400.005356320614.21935813
TAGACCG604.1526012E-412.64522655
GGTATCA45900.012.3533611
ACGGCCC3300.012.14097115
GCCCCCG3350.011.95930218
AGGACCG2300.011.9579865
GTGGTAT15600.011.913191
ACTGCGT1052.7835995E-711.7417728
TGCACGG3500.011.64633312
TGGTATC15750.011.561132
TCGCACG1751.8189894E-1211.4467618
GTGCACG3600.011.32282411
TATCACG855.419588E-511.1573412
AACTGGC4150.011.03344617
ACCGCCT951.3871553E-510.9811728
TATAACG1303.3527613E-810.9427762
TTAGACT2350.010.8964194
ACCGAGT2100.010.8385588
ATCACGC1159.0201684E-710.7207493
ATTAGCC1605.620677E-1010.6692063
Produced by FastQC (version 0.11.5)