FastQCFastQC Report
Thu 26 May 2016
SRR1364239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909081
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN62090.21343510201331625No Hit
GTATCAACGCAGAGTACTTTTTTTT60020.20631945277563601No Hit
TATCAACGCAGAGTACTTTTTTTTT42380.1456817462284481No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA38240.13145044775308765No Hit
GGTATCAACGCAGAGTACTTTTTTT34390.11821602767334426No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31030.10666598833102274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA707.2503735E-613.57457215
ACATGGT14000.012.7614173
GGTATCA32500.012.6591991
TATCACG1301.9826984E-1012.4255252
TACATGT21200.012.0566222
GGACCGA1501.7826096E-1011.3971346
GCGCCGA1553.1286618E-1011.03443119
CATGGTA11000.010.9718574
GTATCAA79400.010.6983981
CGAGGTT909.488396E-510.55909254
TACAACG1451.4406396E-810.4848242
TTGCGCG5550.010.44367918
ACATGTG18200.010.3908443
AGGACCG1752.3646862E-1010.3116925
GCGCCAC1252.58185E-69.87700513
ATCACGC1752.561137E-99.7747033
ATGGTAT5150.09.7742415
TGCGCGC5950.09.74141519
TAACGCA2054.1836756E-119.7349694
GTATAGA3950.09.6219831
Produced by FastQC (version 0.11.5)