FastQCFastQC Report
Thu 26 May 2016
SRR1364239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909081
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT194710.6693179048641135No Hit
TATCAACGCAGAGTACTTTTTTTTT161790.5561550194030348No Hit
GGTATCAACGCAGAGTACTTTTTTT138190.47502974307006235No Hit
GTACTTTTTTTTTTTTTTTTTTTTT92150.31676670398658546No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68200.23443829855545445No Hit
GAGTACTTTTTTTTTTTTTTTTTTT63090.2168726137223405No Hit
GTGGTATCAACGCAGAGTACATGGG44030.15135364054833814No Hit
GGTATCAACGCAGAGTACATGGGAT29660.10195659728965951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1550.013.4525522
ATAACGC2200.012.9235683
GGTATCA55250.012.26345351
TATAACG2350.012.099492
CGCTCGG657.65754E-411.75817219
AGGACCG2450.011.6050365
TAACGCA2550.011.5214034
GCAACGC600.00598341111.0568293
ACGTGCC951.3978104E-510.9736968
TACGCTG700.001521956310.8313665
CGACCGT803.5819196E-410.74770419
ATCACGC2050.010.6330483
ATAAACG2200.010.3388553
GTGGTAT17650.010.3085011
CGGTTTG1502.2466338E-810.18935614
TGGTATC17350.010.1607832
CGGTATA750.002700340510.1097972
TAAACGC1804.129106E-1010.00379854
AGTGACG1252.3936518E-69.93565619
GGACCGA2201.8189894E-129.9080696
Produced by FastQC (version 0.11.5)