FastQCFastQC Report
Thu 26 May 2016
SRR1364238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472853
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN32310.219370161176981No Hit
GTATCAACGCAGAGTACTTTTTTTT15440.10483055674938369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGT10350.013.586833
TCCGGTG852.704328E-713.40945612
CGAAACC957.369272E-813.00219218
CTCAACG1501.8189894E-1212.6688014
CAGTCCG604.1000865E-412.6636259
GTACTAT550.0030691312.0904761
CATGGTA8200.011.7031914
CTACACT1251.8264473E-811.4019214
TCGGTTT1850.011.2949713
CAACGGA1354.7130015E-911.2611574
GTCCTAT1104.96917E-711.2268711
TATTAGC1803.6379788E-1211.083692
CGACCGT951.357403E-511.00185519
CTACACG700.001489221410.8589734
ACGAAAC1053.4581917E-610.85897317
CATTCCG1158.8406705E-710.7365519
TACATGT12650.010.6643752
GGTATCA19350.010.456991
CGGGATT1002.3936684E-510.4517614
TTTACAC2650.010.3979793
Produced by FastQC (version 0.11.5)