FastQCFastQC Report
Thu 26 May 2016
SRR1364238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472853
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48970.3324839614000854No Hit
TATCAACGCAGAGTACTTTTTTTTT44490.30206680503756994No Hit
GGTATCAACGCAGAGTACTTTTTTT36720.24931204947133218No Hit
GTGGTATCAACGCAGAGTACATGGG24310.16505381052963194No Hit
GGTATCAACGCAGAGTACATGGGAT21590.14658625130953326No Hit
GGTCGGGAGTGGGTAATTTGCGCGC20100.13646983100146448No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18900.12832237840436214No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18560.1260139335018498No Hit
GGTATCAACGCAGAGTACATGGGAG16690.11331748653803198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG350.00221819316.2323842
ACGAAAC851.555236E-814.63843817
ACCGTCC400.005393830614.2038178
GTTCGAC1550.013.58505216
AAGTTCG1600.012.56231314
TCGACCG1600.012.56231318
TTCGACC1800.012.22997817
CGACCGT1650.012.18163819
ATCTCCG802.97529E-511.83169210
CGACCTC657.5350975E-411.78004514
CTACACG1052.837496E-711.7233884
AGGACCG658.2467694E-411.6540195
GATAGTC2000.011.3630547
GGTACAT2650.011.0753111
GGGTATC3250.011.0698141
TGTCCGA600.006037353511.042912510
GGTATCA29250.011.0374461
TAGATAG2350.010.879155
CGAAGAG803.8852077E-410.6528638
AAACGAA1355.0931703E-810.63476315
Produced by FastQC (version 0.11.5)