FastQCFastQC Report
Thu 26 May 2016
SRR1364237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939714
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN58610.19937313629829295No Hit
GTATCAACGCAGAGTACTTTTTTTT42600.14491205607076063No Hit
TATCAACGCAGAGTACTTTTTTTTT30070.10228886211379747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACG1950.012.6708774
ACGAAAC1207.403287E-1012.66741717
ACATGGT15700.012.6507013
TATCACG1103.8073267E-812.0934832
GGTATCA32150.011.9998531
TACATGT18350.011.4956352
ACGCTAT1104.984886E-711.22525610
TTGACCG2150.011.04735618
TGCGCGC6250.010.79319219
CATGGTA12700.010.7752334
CCTATAC2300.010.74273
GTATCAA75550.010.66577151
TTGCGCG6150.010.50486718
TAACGCA1303.8274084E-710.2341694
TTTGCGC6550.010.15327417
ACATGTG17050.010.0883953
GGTCCGA856.6075573E-410.0570176
ACGGCCC1803.929017E-1010.02888615
TCAACTC16150.09.8856384
ATTTGCG6650.09.85823216
Produced by FastQC (version 0.11.5)