FastQCFastQC Report
Thu 26 May 2016
SRR1364237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939714
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT129960.44208382175953176No Hit
TATCAACGCAGAGTACTTTTTTTTT109140.37126060562354024No Hit
GGTATCAACGCAGAGTACTTTTTTT87520.2977160363219007No Hit
GTACTTTTTTTTTTTTTTTTTTTTT54450.18522209983692292No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45260.15396055534654052No Hit
GTGGTATCAACGCAGAGTACATGGG44050.14984450868349777No Hit
GAGTACTTTTTTTTTTTTTTTTTTT35500.12076004672563385No Hit
GGTATCAACGCAGAGTACATGGGAT31230.1062348242039872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG951.8189894E-1115.9681712
TCGACCG400.005111970514.31986518
ATAACGC2050.012.9482533
TACCGTG751.5118321E-512.6401777
GGTATCA47100.012.2986351
ACCGCGT658.1973796E-411.66309210
TAACGCA2150.011.46454054
TAATACC3100.011.0093744
AGGACCG2350.010.8922525
TATAACG2400.010.6662392
AATACCG803.8459082E-410.6653315
CGACCTA1002.2742171E-510.50123518
CGAAATC1553.8235157E-910.39296613
ACCGAGT1856.184564E-1110.2487928
GTCGGTT1951.6370905E-1110.20485812
CTGGTCG2151.8189894E-1210.1375689
AACGCCG2450.010.06071955
ACGACCA856.723785E-410.0379585
TGGTCGG2002.7284841E-119.95007510
GTGGTAT15100.09.857331
Produced by FastQC (version 0.11.5)