##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364237_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2939714 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.95556778652617 31.0 28.0 33.0 25.0 34.0 2 29.031801052755473 31.0 28.0 34.0 24.0 34.0 3 29.114064837599848 31.0 28.0 34.0 25.0 34.0 4 32.58945734176862 35.0 32.0 37.0 26.0 37.0 5 31.791460665901514 35.0 32.0 37.0 20.0 37.0 6 31.808267062714265 35.0 32.0 37.0 19.0 37.0 7 31.387049216352338 35.0 32.0 37.0 17.0 37.0 8 31.19931564771267 35.0 32.0 37.0 16.0 37.0 9 31.586789054989705 37.0 30.0 39.0 10.0 39.0 10 31.528532367434384 37.0 30.0 39.0 10.0 39.0 11 32.13412189076897 37.0 31.0 39.0 15.0 39.0 12 31.967377778926792 37.0 31.0 39.0 10.0 39.0 13 32.06671635403988 37.0 32.0 39.0 10.0 39.0 14 32.49320444097623 38.0 31.0 40.0 10.0 41.0 15 32.76824242086135 38.0 31.0 40.0 10.0 41.0 16 32.79110042677621 38.0 32.0 40.0 10.0 41.0 17 32.78736604989465 38.0 32.0 40.0 10.0 41.0 18 32.67353797002021 38.0 32.0 40.0 8.0 41.0 19 32.544329482391824 38.0 31.0 40.0 2.0 41.0 20 32.23819494005199 38.0 31.0 40.0 2.0 41.0 21 31.776655824342097 38.0 30.0 40.0 2.0 41.0 22 31.821064225975725 38.0 30.0 40.0 2.0 41.0 23 31.68911295452551 38.0 30.0 40.0 2.0 41.0 24 31.690628408069628 38.0 31.0 40.0 2.0 41.0 25 31.57460691754368 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0777936015692653 1101 2 1.096002938217243 1101 3 1.1051941033774781 1101 4 1.1602209437216757 1101 5 1.500712350391371 1101 6 1.3082264831107118 1101 7 1.6418895282167192 1101 8 1.7469640070847667 1101 9 2.600145049293481 1101 10 2.7320028852935963 1101 11 1.9902561418628402 1101 12 1.9953442436624726 1101 13 1.6979748045920218 1101 14 2.2662768679892125 1101 15 2.0122128540517252 1101 16 1.8593134130191586 1101 17 1.8485897212267304 1101 18 1.825930199528976 1101 19 1.9235318842578764 1101 20 2.6408141544379724 1101 21 2.945748249936763 1101 22 2.8720603805622495 1101 23 2.727876976968048 1101 24 2.417822671908855 1101 25 2.633144899770784 1102 1 0.31285700788300375 1102 2 0.5294318492065031 1102 3 0.46818235373809003 1102 4 0.4110003011988965 1102 5 0.8014860840870064 1102 6 0.6244859715487472 1102 7 0.8891226033609279 1102 8 0.8644193775669216 1102 9 2.013551977861866 1102 10 2.030851541932307 1102 11 1.354360186615775 1102 12 1.34635071299126 1102 13 1.04973442297797 1102 14 1.5355985871309557 1102 15 1.0920460635970244 1102 16 0.9985914528502633 1102 17 1.0705696897266108 1102 18 1.0273850325735268 1102 19 1.1378749032552804 1102 20 1.7110741176496198 1102 21 2.1717138435090177 1102 22 2.114301072995307 1102 23 1.9590326366424904 1102 24 1.8139152656837645 1102 25 1.9434548163352616 1103 1 0.5438664614180375 1103 2 0.7108549168667402 1103 3 0.7093378976463924 1103 4 0.7678478753361304 1103 5 1.0358335390726658 1103 6 0.7576869564226136 1103 7 1.076526587930136 1103 8 1.0210303075410856 1103 9 1.8353807991582656 1103 10 1.9561053347834658 1103 11 1.304643261229689 1103 12 1.342656381555301 1103 13 1.066854570925564 1103 14 1.8501141027746826 1103 15 1.4060363984712865 1103 16 1.1675620212166251 1103 17 1.1776163067428058 1103 18 0.9790214441133571 1103 19 1.091728518201542 1103 20 1.634578174918854 1103 21 2.329726966068961 1103 22 1.904209012061127 1103 23 1.9581486655307359 1103 24 1.9163485024914344 1103 25 1.7110290407358448 1104 1 0.5126450451476927 1104 2 0.6544606438771545 1104 3 0.41210773945933 1104 4 0.4617546225219513 1104 5 0.8277069922723364 1104 6 0.6829017977223266 1104 7 0.8951547684937715 1104 8 0.9363088456508244 1104 9 1.6851082201502692 1104 10 1.7769836465043625 1104 11 1.1615264514305288 1104 12 0.9613850685000216 1104 13 0.9971030711707698 1104 14 1.579674455074496 1104 15 0.6301346210052472 1104 16 0.7339953847731522 1104 17 0.36344177064034255 1104 18 0.9636620019308992 1104 19 1.1748615515839163 1104 20 1.6631434427647953 1104 21 2.2351629356632827 1104 22 2.1826071256775315 1104 23 2.01491516853595 1104 24 1.7920233921482627 1104 25 1.6659160744147528 1105 1 -0.07855606571296292 1105 2 0.2875337095520223 1105 3 0.1614665526532555 1105 4 0.5005433538173563 1105 5 0.6033494433904174 1105 6 0.5669146084540948 1105 7 0.4567889676279684 1105 8 0.764641499867988 1105 9 1.6205947852095974 1105 10 1.6085487130577505 1105 11 0.8399136516802095 1105 12 1.018021911852486 1105 13 0.6742165312531867 1105 14 0.7631554961740292 1105 15 0.9468355685890373 1105 16 0.34551099144062647 1105 17 0.7165828274028954 1105 18 0.5333574905766554 1105 19 0.7061438818482273 1105 20 1.2584494857427408 1105 21 1.9177593918328384 1105 22 1.2537220032343335 1105 23 1.4508483247211075 1105 24 1.2058886323819955 1105 25 0.8541725664763149 1106 1 0.14567891221770424 1106 2 0.12701395234913448 1106 3 -0.14932304862808365 1106 4 0.06418925281802501 1106 5 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1107 17 0.28412447487567505 1107 18 0.4404019895261513 1107 19 0.566326641075328 1107 20 0.8599636182033983 1107 21 1.5983839617132602 1107 22 1.2627993992908593 1107 23 1.2659906159929761 1107 24 0.8920145316130821 1107 25 1.1154198844448757 1108 1 -0.12729344870169257 1108 2 0.09113968678144246 1108 3 0.19566834551993395 1108 4 -0.9778335862989316 1108 5 0.16842668924202897 1108 6 0.1321509726398169 1108 7 0.34012701571849036 1108 8 0.4826672314894047 1108 9 1.2616128706153162 1108 10 1.0854396171014216 1108 11 0.5899467934647298 1108 12 0.7483307630694824 1108 13 0.36482771863551733 1108 14 0.9526578784168294 1108 15 0.7830835408972376 1108 16 0.5868910493396058 1108 17 0.4100206785311329 1108 18 0.3914976604967606 1108 19 0.6070855239811621 1108 20 1.2555898124017624 1108 21 1.5705616005297713 1108 22 1.6699323582007857 1108 23 1.1066167136904639 1108 24 1.0878379736064083 1108 25 0.8311695997672857 1109 1 0.08918299847997346 1109 2 0.18520096479811343 1109 3 -0.2893634893247494 1109 4 0.21582496612779778 1109 5 0.33267519396902046 1109 6 0.041695771611323096 1109 7 0.20092501679971164 1109 8 0.2195631765344217 1109 9 1.2314803925787814 1109 10 1.4030422135563256 1109 11 0.33033225010802525 1109 12 0.45114032899616774 1109 13 0.21481726939456536 1109 14 0.7577834316821708 1109 15 0.3859836159713055 1109 16 0.30049450924184384 1109 17 -0.30502061220801835 1109 18 0.28945476703125905 1109 19 0.41456821205316885 1109 20 0.8645836479226894 1109 21 1.1624604014459408 1109 22 1.1121479029271661 1109 23 0.6553559086595477 1109 24 0.641739246427111 1109 25 0.8187639629080152 1110 1 0.26498114377656634 1110 2 0.3224343447643534 1110 3 0.21213222384693253 1110 4 0.39914228239059923 1110 5 0.5535977919036306 1110 6 0.3254081348579412 1110 7 0.36987967919776565 1110 8 0.5754824255563413 1110 9 1.3064067568480482 1110 10 1.1306570247737469 1110 11 0.16818908537453225 1110 12 0.38315024882033555 1110 13 0.11201478334959347 1110 14 1.0241705087673196 1110 15 0.1896468055887226 1110 16 0.4105472628697058 1110 17 0.20570038338374275 1110 18 0.5393698887842291 1110 19 0.3747909789372983 1110 20 1.172514482055039 1110 21 1.2315716521052735 1110 22 1.406495246428765 1110 23 1.2510879707021019 1110 24 0.8666863717542093 1110 25 1.1855727168850834 1111 1 -0.16036298306707408 1111 2 -0.09743126526016255 1111 3 -0.22082476320900923 1111 4 -0.017180014816212008 1111 5 0.04426895570450995 1111 6 -0.34350836052167466 1111 7 0.14679115533306586 1111 8 0.08694982667082485 1111 9 1.0129575403043347 1111 10 0.93515774971101 1111 11 0.029774397970971478 1111 12 0.2530691373663281 1111 13 0.12337024134107821 1111 14 0.08605613171842208 1111 15 0.05591859009894051 1111 16 0.10429708090754275 1111 17 -0.8083069183939529 1111 18 -0.36814776882412303 1111 19 0.03006074876244469 1111 20 0.3882614876856785 1111 21 1.0532156875311216 1111 22 1.0459348903069774 1111 23 0.6880685051176947 1111 24 0.7465479640541481 1111 25 0.7443048627575983 1112 1 0.13516708345035156 1112 2 0.2657929998343391 1112 3 0.11159989714462171 1112 4 0.3011419344025299 1112 5 0.3497572026048452 1112 6 -0.22617257900009946 1112 7 0.17549690840858645 1112 8 0.228975538536222 1112 9 1.0131258507923917 1112 10 1.1729405356224234 1112 11 0.5053217879916794 1112 12 0.2652237825002466 1112 13 -0.2450002466643788 1112 14 0.6995541911974996 1112 15 0.09176404978642694 1112 16 -0.41765461114837876 1112 17 -0.36812769735241346 1112 18 -0.13143840853794586 1112 19 0.2694459992134526 1112 20 0.6230938529423291 1112 21 1.1520988905787135 1112 22 1.1284105259560313 1112 23 0.8564383968778202 1112 24 0.402429607736174 1112 25 0.9227236230796336 1113 1 -1.0105011474006638 1113 2 -0.9932515698887876 1113 3 -1.1572897958780217 1113 4 -1.1468758692574497 1113 5 -1.3774775767303318 1113 6 -1.356118664257778 1113 7 -0.9580775597961413 1113 8 -1.1866410872855901 1113 9 -10.106773444483249 1113 10 -11.759383214070137 1113 11 -5.382765102199098 1113 12 -2.8604640050514334 1113 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22 3.012792304750892 2203 23 2.9782879483397977 2203 24 2.665924776659555 2203 25 2.863118723523371 2204 1 1.110084348062145 2204 2 1.1717428667062073 2204 3 1.1768589517460413 2204 4 1.2477787772312467 2204 5 1.468317459313031 2204 6 1.4630924949733703 2204 7 1.8120463081392693 2204 8 1.5887365221978307 2204 9 2.643851579467082 2204 10 2.8770876011242947 2204 11 1.948931495867253 2204 12 2.2026714991638414 2204 13 1.705033308828618 2204 14 2.5520712452040826 2204 15 2.1282298206808647 2204 16 2.1899193499850753 2204 17 2.133697509349112 2204 18 2.2227408088421754 2204 19 2.18362787401081 2204 20 2.823719403077927 2204 21 3.0866066119123943 2204 22 3.04446974421683 2204 23 3.0497296224690587 2204 24 2.701782093305109 2204 25 2.955576468635492 2205 1 0.399129446250857 2205 2 0.4786948212894728 2205 3 0.5428483118813645 2205 4 0.4862753153406274 2205 5 0.7659037963248174 2205 6 0.4806086126478881 2205 7 0.40462248339271056 2205 8 0.9571015465336963 2205 9 1.5430399010028992 2205 10 1.7926736124127487 2205 11 1.2281941091325699 2205 12 1.290319620523718 2205 13 1.1057843641038403 2205 14 1.2726524385157134 2205 15 0.407125646472025 2205 16 0.35103832633604526 2205 17 0.3299909996148145 2205 18 0.8421345544982515 2205 19 0.8606619259121402 2205 20 1.4803765923318544 2205 21 1.5729801731663287 2205 22 1.0580952813890114 2205 23 0.25071133096381715 2205 24 0.5456709686420353 2205 25 0.7108986038768172 2206 1 -1.2100046851787347 2206 2 -3.043895704700688 2206 3 -0.8748602626764601 2206 4 -2.0185271848609254 2206 5 -0.40371744063324044 2206 6 -0.8814856173110002 2206 7 -0.7819473504985694 2206 8 -0.03675446914432712 2206 9 1.1173487605773076 2206 10 0.636177183037784 2206 11 0.46550991105148753 2206 12 0.26635468147795294 2206 13 0.23509311064690053 2206 14 0.7134823793979095 2206 15 0.019600140884300288 2206 16 0.3474857752148921 2206 17 0.5745305589390881 2206 18 -0.10759935195641646 2206 19 0.386532432837555 2206 20 0.9506884008589047 2206 21 1.4613177099953987 2206 22 1.4547334073019336 2206 23 1.1097041917116783 2206 24 0.8778830778990603 2206 25 0.7646313003226126 2207 1 0.3763259720639134 2207 2 0.43600722018792837 2207 3 0.20165765033276983 2207 4 0.32740785873807 2207 5 0.6689430086224455 2207 6 0.5155828168384353 2207 7 0.4893223571593275 2207 8 0.6889311641707074 2207 9 1.0582380990610787 2207 10 1.5538595300866405 2207 11 0.3408321862636505 2207 12 0.6351050267027389 2207 13 0.6003089381570632 2207 14 1.6175774454242386 2207 15 0.854577964173231 2207 16 0.1526216832549281 2207 17 0.23638955476966572 2207 18 0.551879240236886 2207 19 0.7831695299476777 2207 20 1.4235278394701858 2207 21 1.576461955271128 2207 22 1.412552113076881 2207 23 0.7826041014758971 2207 24 1.1186599401844042 2207 25 0.9711868347565016 2208 1 0.2842289655323498 2208 2 0.5135396721674361 2208 3 0.4654469433059134 2208 4 0.3854421434880706 2208 5 0.5066185711608462 2208 6 0.5898974926792739 2208 7 1.105971888028602 2208 8 0.9342108360343637 2208 9 1.829534028044712 2208 10 1.9258298111023748 2208 11 1.204087672497284 2208 12 1.240463677467929 2208 13 1.0428504434172226 2208 14 1.654334105080089 2208 15 1.242636717617124 2208 16 1.171146648331245 2208 17 1.081529940158397 2208 18 0.7742589772188353 2208 19 1.0471121945448942 2208 20 1.7385970098004684 2208 21 2.3259804745204917 2208 22 1.910732475112841 2208 23 1.984125025407245 2208 24 1.6558442576296422 2208 25 1.5666988095148255 2209 1 0.2772042941061308 2209 2 0.22605036620176122 2209 3 0.2588382681148502 2209 4 0.2823385953128863 2209 5 0.5505866597543765 2209 6 0.28432857005308065 2209 7 0.4319614177359128 2209 8 0.4544276954090023 2209 9 1.4179093237033058 2209 10 1.3959017484601546 2209 11 0.39504436182827973 2209 12 0.9327379262332727 2209 13 0.5832475746277481 2209 14 0.986316646431483 2209 15 0.610516326932931 2209 16 0.4509251645093286 2209 17 0.1772924503083786 2209 18 0.30025775582070224 2209 19 0.16566690515650606 2209 20 1.0000713515988089 2209 21 1.683282748796458 2209 22 1.5553575752525255 2209 23 1.3400601634537992 2209 24 0.90975116750597 2209 25 0.8696986476591775 2210 1 0.16802279525147057 2210 2 0.38920379456208565 2210 3 0.477677477940027 2210 4 0.5911288441906919 2210 5 0.43737646093152804 2210 6 0.5775707474940184 2210 7 0.8393069063102878 2210 8 0.8018308464810424 2210 9 1.7739127033383113 2210 10 1.762255024669578 2210 11 0.9692080620573442 2210 12 1.0016631922028054 2210 13 0.9566013069532531 2210 14 1.5223264891135173 2210 15 0.959023682856639 2210 16 0.9705774727764762 2210 17 1.0338101807690236 2210 18 0.808104197862825 2210 19 1.08419841335229 2210 20 1.6173728948195922 2210 21 2.103688776261045 2210 22 1.9204879891063378 2210 23 1.9821987924462903 2210 24 1.9950661484786885 2210 25 1.8574519814301098 2211 1 0.26509795260060187 2211 2 0.13134127450778266 2211 3 0.09098366083524922 2211 4 0.14222350049082877 2211 5 0.33239065320599437 2211 6 0.11337477069529811 2211 7 0.4284491992881989 2211 8 0.40870237554435107 2211 9 1.3149444199864675 2211 10 1.2857500265207449 2211 11 0.538713183617265 2211 12 0.08696246407741981 2211 13 -0.13170425888656112 2211 14 0.5818970185470604 2211 15 0.381042688163312 2211 16 0.4279685624963321 2211 17 0.21861580831437522 2211 18 -0.00643115941225858 2211 19 -0.02225351803114961 2211 20 0.6154970463684002 2211 21 1.4748056465559927 2211 22 1.4836349717053743 2211 23 1.2724591898299735 2211 24 1.050704800829667 2211 25 0.8682010866997629 2212 1 0.2937614664598165 2212 2 0.2741156067832655 2212 3 0.042966887346491234 2212 4 0.2575798161311056 2212 5 0.21585569921900571 2212 6 0.2988233889221199 2212 7 0.4339844505793984 2212 8 0.293480814766383 2212 9 -9.174081528448536 2212 10 -1.8081478391115802 2212 11 -0.754445431636519 2212 12 0.07916106329059858 2212 13 0.2531815555541286 2212 14 0.7929836308692799 2212 15 0.4376395920510845 2212 16 0.308833026346214 2212 17 0.45360927453577915 2212 18 0.5438154648021296 2212 19 0.6475280670842807 2212 20 1.1567335553255553 2212 21 1.5931778022880891 2212 22 1.364651475546129 2212 23 1.1957122510262437 2212 24 1.0101110220931453 2212 25 0.9630264723309807 2213 1 0.839275392976564 2213 2 0.8296476378933448 2213 3 0.6927327594586892 2213 4 0.8974906789681469 2213 5 0.8352165519664219 2213 6 0.8646115830091965 2213 7 1.1912888922018077 2213 8 1.4530871087464057 2213 9 2.1284716325210766 2213 10 -8.204056760202622 2213 11 -1.8541570466975301 2213 12 -0.008527894285165871 2213 13 0.8350904555744663 2213 14 1.468553410947905 2213 15 1.121671891812582 2213 16 1.4662157537213432 2213 17 1.2586135251441988 2213 18 1.3500590372257761 2213 19 1.786135990801867 2213 20 2.5050543016173172 2213 21 2.3227011344409263 2213 22 2.6670324240679797 2213 23 2.3186804480470045 2213 24 2.3297885836621326 2213 25 2.02235426839254 2214 1 -1.5979930257016441 2214 2 -1.8181625955594676 2214 3 -1.723674377484759 2214 4 -1.6507773877213268 2214 5 -1.6531695548894234 2214 6 -1.757532474578145 2214 7 -9.681440408629577 2214 8 -3.387234688280035 2214 9 -2.6375336919282617 2214 10 -2.009537373555389 2214 11 -2.5247838345276428 2214 12 -11.871073318183715 2214 13 -4.788526997117749 2214 14 -13.10213038914232 2214 15 -6.342931426796717 2214 16 -4.222387991240506 2214 17 -3.389071033456972 2214 18 -2.96300519823982 2214 19 -3.1009440395385717 2214 20 -2.9037873084089085 2214 21 -12.056479723320923 2214 22 -14.772269101381795 2214 23 -8.966606918531912 2214 24 -7.315467004778753 2214 25 -15.328428782606162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 124283.0 3 4.0 4 2134.0 5 19593.0 6 23808.0 7 18786.0 8 15734.0 9 12229.0 10 10539.0 11 9527.0 12 8921.0 13 8726.0 14 8443.0 15 8579.0 16 8949.0 17 9637.0 18 11057.0 19 13378.0 20 16863.0 21 21897.0 22 27664.0 23 33162.0 24 38630.0 25 44522.0 26 50207.0 27 55239.0 28 62006.0 29 71295.0 30 84207.0 31 101457.0 32 124499.0 33 150951.0 34 182491.0 35 217982.0 36 268929.0 37 359473.0 38 713913.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.75187084221841 19.608141366360464 13.539511323388853 24.100476468032273 2 16.18743340359835 22.216049273512358 37.90690996778878 23.68960735510052 3 18.455726198117954 27.340966155079155 29.835257186768732 24.36805046003416 4 12.578978771404293 17.855138288962806 37.08935631153235 32.47652662810056 5 13.09726987129223 39.18329163271936 33.506202363923045 14.213236132065363 6 29.81611491389146 37.41015123507396 18.94615775555149 13.827576095483085 7 27.73748997340529 32.91536295422431 21.80460335505677 17.54254371731364 8 26.877124394755935 33.94767505965661 20.527625809771298 18.647574735816157 9 26.94504525528772 15.814346841314952 20.038094317966106 37.20251358543122 10 15.027738241353317 30.56248009949572 31.73052063801357 22.6792610211374 11 33.90118224653717 23.737086985082527 23.24735599354056 19.114374774839746 12 23.583818017847005 27.25598996578037 29.19486409612929 19.965327920243332 13 28.386003382031827 22.126863272637962 26.62589768248157 22.861235662848642 14 22.529275454609778 21.53368548242248 28.10423232042846 27.832806742539283 15 24.250911995341035 29.068873507164657 23.961835665310975 22.718378832183333 16 23.163745329869478 28.712760141960402 25.583079593685465 22.540414934484655 17 22.11127107066463 28.576978342867402 26.796959619435356 22.514790967032607 18 21.541358243074075 27.267015457464762 29.239407010286833 21.952219289174334 19 23.21433005847261 26.735171024473182 28.662226027641907 21.388272889412306 20 23.58394905235642 26.10305973031387 28.55479856469873 21.75819265263098 21 24.44163724035583 25.503025758497966 27.803228224218113 22.252108776928097 22 24.04754882299892 26.10701580889845 27.56394097457874 22.2814943935239 23 22.935551359902025 26.117569015665666 28.17662567399772 22.77025395043459 24 23.022578786268625 26.391027267760713 28.03237242921125 22.55402151675941 25 22.82001085142897 26.160618157264082 28.569227184360336 22.450143806946617 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 998.0 1 998.0 2 1340.0 3 1682.0 4 1682.0 5 1682.0 6 4424.0 7 7166.0 8 7166.0 9 7166.0 10 7425.5 11 7685.0 12 7685.0 13 7685.0 14 8968.5 15 10252.0 16 10252.0 17 10252.0 18 19781.5 19 29311.0 20 29311.0 21 29311.0 22 49402.5 23 69494.0 24 69494.0 25 69494.0 26 105525.0 27 141556.0 28 141556.0 29 141556.0 30 172889.0 31 204222.0 32 204222.0 33 204222.0 34 236368.0 35 268514.0 36 268514.0 37 268514.0 38 291286.0 39 314058.0 40 314058.0 41 314058.0 42 336081.5 43 358105.0 44 358105.0 45 358105.0 46 374820.5 47 391536.0 48 391536.0 49 391536.0 50 378803.5 51 366071.0 52 366071.0 53 366071.0 54 328479.0 55 290887.0 56 290887.0 57 290887.0 58 249797.5 59 208708.0 60 208708.0 61 208708.0 62 171090.5 63 133473.0 64 133473.0 65 133473.0 66 102154.5 67 70836.0 68 70836.0 69 70836.0 70 52921.0 71 35006.0 72 35006.0 73 35006.0 74 25317.0 75 15628.0 76 15628.0 77 15628.0 78 12027.0 79 8426.0 80 8426.0 81 8426.0 82 6007.0 83 3588.0 84 3588.0 85 3588.0 86 2566.5 87 1545.0 88 1545.0 89 1545.0 90 1218.5 91 892.0 92 892.0 93 892.0 94 482.0 95 72.0 96 72.0 97 72.0 98 37.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027111480912769065 2 0.012450190732839997 3 0.0013946934973946446 4 0.0 5 0.0037078436881955185 6 0.0017688795576712565 7 0.0010885412662592347 8 0.041840804921839336 9 0.005714841647860982 10 0.003061522311354098 11 0.004796384954454753 12 0.005884926220713987 13 0.0020750317888066664 14 0.001700845728530054 15 2.041014874236065E-4 16 7.143552059826227E-4 17 0.002789386994789289 18 0.0011565750954004369 19 0.001598794984818251 20 0.734119033348142 21 0.015341628471341089 22 0.005680824733290382 23 0.005578773989578578 24 0.0017688795576712565 25 3.0615223113540977E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2939714.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.51026928617484 #Duplication Level Percentage of deduplicated Percentage of total 1 73.34124292870989 33.37779715468353 2 13.378228067328196 12.176935238319372 3 5.08436201837283 6.9417205381364075 4 2.4828399582020775 4.519788603690065 5 1.4267633459767302 3.2466192041522417 6 0.8823879770277704 2.409462866965013 7 0.6430616425762011 2.048613596487698 8 0.423792441236143 1.5429526497681836 9 0.3164726964258229 1.2962481872454954 >10 1.767951121474039 14.778697044137656 >50 0.14756875402761202 4.675362406449775 >100 0.09751763216221561 8.293491298348087 >500 0.0053898773714513275 1.6167413879814347 >1k 0.002109082449687865 1.7208672405934673 >5k 1.5622832960650855E-4 0.5047028779867676 >10k+ 1.5622832960650855E-4 0.8499997050548433 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 12996 0.44208382175953176 No Hit TATCAACGCAGAGTACTTTTTTTTT 10914 0.37126060562354024 No Hit GGTATCAACGCAGAGTACTTTTTTT 8752 0.2977160363219007 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5445 0.18522209983692292 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4526 0.15396055534654052 No Hit GTGGTATCAACGCAGAGTACATGGG 4405 0.14984450868349777 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 3550 0.12076004672563385 No Hit GGTATCAACGCAGAGTACATGGGAT 3123 0.1062348242039872 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.401691457060108E-5 0.0 0.0 0.0 0.0 6 3.401691457060108E-5 0.0 0.0 0.0 0.0 7 6.803382914120217E-5 0.0 0.0 0.0 0.0 8 6.803382914120217E-5 0.0 0.0 0.0 0.0 9 1.0205074371180326E-4 0.0 0.0 0.0 0.0 10 1.0205074371180326E-4 0.0 0.0 3.401691457060108E-5 0.0 11 1.0205074371180326E-4 3.401691457060108E-5 0.0 6.803382914120217E-5 0.0 12 1.0205074371180326E-4 3.401691457060108E-5 0.0 6.803382914120217E-5 3.401691457060108E-5 13 1.0205074371180326E-4 3.401691457060108E-5 0.0 1.0205074371180326E-4 3.401691457060108E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 95 1.8189894E-11 15.968171 2 TCGACCG 40 0.0051119705 14.319865 18 ATAACGC 205 0.0 12.948253 3 TACCGTG 75 1.5118321E-5 12.640177 7 GGTATCA 4710 0.0 12.298635 1 ACCGCGT 65 8.1973796E-4 11.663092 10 TAACGCA 215 0.0 11.4645405 4 TAATACC 310 0.0 11.009374 4 AGGACCG 235 0.0 10.892252 5 TATAACG 240 0.0 10.666239 2 AATACCG 80 3.8459082E-4 10.665331 5 CGACCTA 100 2.2742171E-5 10.501235 18 CGAAATC 155 3.8235157E-9 10.392966 13 ACCGAGT 185 6.184564E-11 10.248792 8 GTCGGTT 195 1.6370905E-11 10.204858 12 CTGGTCG 215 1.8189894E-12 10.137568 9 AACGCCG 245 0.0 10.0607195 5 ACGACCA 85 6.723785E-4 10.037958 5 TGGTCGG 200 2.7284841E-11 9.950075 10 GTGGTAT 1510 0.0 9.85733 1 >>END_MODULE