FastQCFastQC Report
Thu 26 May 2016
SRR1364236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10230227
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN204080.19948726455434468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52550.013.0191171
GGACCGA10400.012.5113626
AGGACCG11900.011.8918735
GTATCAA132450.010.8975181
ACCGAGT10300.010.235468
CGAGTGG10100.09.68584310
ACGGCCC4550.09.39745215
GTCCTAC9100.09.1888911
CAGGACC24950.09.1793734
TAGGACA13150.09.1056584
ACATGGT39100.09.0656423
TCGCGCA7700.09.00795618
GTATTAA16900.08.9961161
TGGTATC18600.08.9402362
TACATGT47550.08.7927782
GACCGAG14050.08.7203297
GTATAAT17250.08.6483321
GTTCGCG7850.08.59404716
CTAGGAC16600.08.5871623
CCAGGAC37300.08.3056813
Produced by FastQC (version 0.11.5)