FastQCFastQC Report
Thu 26 May 2016
SRR1364236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10230227
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT201680.19714127555527358No Hit
TATCAACGCAGAGTACTTTTTTTTT149910.14653633785447773No Hit
GGTATCAACGCAGAGTACTTTTTTT125480.12265612483476661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73400.013.3077121
GGACCGA11600.012.1583796
AGGACCG13950.011.6029745
TGGTATC23500.011.4403772
ACCGAGT12150.011.3742818
CGAGTGG12350.011.03204610
GGCGCTA2400.010.2497712
GTATCAA158050.010.2285271
GTGGTAT30300.010.0897331
CCGAGTG14150.09.9631519
CGCGCCA4900.09.65460310
GCGCTAG2357.2759576E-129.25988613
GTATTAG8600.09.024751
CGATACT4200.09.0148414
CTTACAC14950.08.9272763
TAGCACA15950.08.9018034
TAATACT13400.08.7591964
GTTCGCG7700.08.70838816
ATAACGC2304.3473847E-108.6423633
TAGGACA10850.08.6367994
Produced by FastQC (version 0.11.5)