FastQCFastQC Report
Thu 26 May 2016
SRR1364235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958289
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT97430.3293457806184588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA77610.26234759349069686No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN64390.2176595998565387No Hit
TATCAACGCAGAGTACTTTTTTTTT62200.21025667201547923No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52100.1761153153055702No Hit
GGTATCAACGCAGAGTACTTTTTTT51740.17489839566046456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3300.013.822292
GGTATCA35700.013.4416871
TATCACG1500.013.3039542
ATAACGC3650.012.7578663
GCGCCGA951.0398471E-612.00092119
CGACCGT658.022003E-411.69320519
TAACGCA4250.011.6275784
CGGCGGA752.0732135E-411.40106818
CGTCGCC600.005871453311.08493614
GTATCAA88850.011.0584361
ACATGGT13250.011.0453553
AACGGAG2850.010.9976735
CTCAACG3450.010.742874
CGTCTCG1158.8122397E-710.74031917
TGAATCG803.780219E-410.685245
CATGGTA10100.010.6324154
ATCACGC1803.45608E-1110.5592313
TCACGCA2001.8189894E-1210.453644
TACATGT19050.010.4256722
CAACGGA2900.010.158714
Produced by FastQC (version 0.11.5)