##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364235_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2958289 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.200796473907722 28.0 16.0 31.0 2.0 31.0 2 23.862885607187128 28.0 16.0 31.0 2.0 34.0 3 23.47940414205644 28.0 16.0 31.0 2.0 34.0 4 26.297313413260166 32.0 19.0 35.0 2.0 37.0 5 26.7745483960492 32.0 22.0 35.0 2.0 37.0 6 27.284150061065702 33.0 25.0 35.0 2.0 37.0 7 27.176892115679028 33.0 25.0 35.0 2.0 37.0 8 25.55400807696611 32.0 17.0 35.0 2.0 37.0 9 25.28694627198357 32.0 11.0 37.0 2.0 39.0 10 26.84186636261704 32.0 17.0 37.0 2.0 39.0 11 27.267093918139842 34.0 19.0 37.0 2.0 39.0 12 25.982054829666744 32.0 12.0 37.0 2.0 39.0 13 25.748111154792518 32.0 12.0 37.0 2.0 39.0 14 26.177605365804354 32.0 13.0 38.0 2.0 40.0 15 25.73303081612378 32.0 11.0 38.0 2.0 40.0 16 26.433818332150782 32.0 17.0 38.0 2.0 40.0 17 26.95193606845038 33.0 17.0 38.0 2.0 40.0 18 27.46972692661197 34.0 17.0 39.0 2.0 40.0 19 28.079575389693165 34.0 22.0 40.0 2.0 41.0 20 28.095839182716766 34.0 20.0 40.0 2.0 41.0 21 28.243045557753145 35.0 22.0 40.0 2.0 41.0 22 28.126596150680342 35.0 22.0 40.0 2.0 41.0 23 27.794615400996996 34.0 18.0 40.0 2.0 41.0 24 27.73139676346699 34.0 18.0 40.0 2.0 41.0 25 27.441546109930435 34.0 16.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.8380291227904593 1101 2 -0.9913768291205258 1101 3 -3.1837238972649864 1101 4 -3.419779158030252 1101 5 -4.02693175548842 1101 6 -2.2132031659251368 1101 7 -1.8754079849806082 1101 8 -5.212738106469988 1101 9 -6.398742688858263 1101 10 -6.665605329212344 1101 11 -3.7773062721723107 1101 12 -1.3936514159937978 1101 13 -0.7257919491972942 1101 14 -7.101220748656644 1101 15 -8.872054762121593 1101 16 -4.469537009294029 1101 17 -2.72016054210194 1101 18 -7.017471590411766 1101 19 -3.87534786131733 1101 20 -2.7562238024475008 1101 21 -2.6668695939770473 1101 22 -2.6773127601578786 1101 23 -7.132130660255644 1101 24 -4.3267007551540395 1101 25 -3.4210597570261427 1102 1 1.3166375349370476 1102 2 0.909366705784759 1102 3 1.2773959396847445 1102 4 1.4097800215418168 1102 5 0.9402504568844741 1102 6 0.31268067823036816 1102 7 0.22380419064218415 1102 8 1.5904711736777841 1102 9 -3.345372656212046 1102 10 -0.8946502633195657 1102 11 -0.3095823264191928 1102 12 -4.104528219545408 1102 13 -6.0288350422439905 1102 14 -6.17714529646215 1102 15 -1.6021543138836947 1102 16 -0.09887262861423451 1102 17 -4.352949927581456 1102 18 -1.9012251353471967 1102 19 -1.1517160961766812 1102 20 -0.7530123078261326 1102 21 -0.5346850239008418 1102 22 -0.5542309971199622 1102 23 -0.30749167532157884 1102 24 -0.26600630029750505 1102 25 -0.0317992835310541 1103 1 -1.3204240149320405 1103 2 0.4447920349361105 1103 3 1.2639724929311704 1103 4 2.017711633269222 1103 5 1.6846636305523113 1103 6 0.8826913929024833 1103 7 0.922525032254832 1103 8 2.339922539680579 1103 9 3.3107899820810047 1103 10 1.8524235690890727 1103 11 1.4746822238041304 1103 12 2.8419542247476244 1103 13 2.908429927148081 1103 14 -2.9447436686958888 1103 15 1.0592577414312707 1103 16 1.634120469283367 1103 17 1.6963222887208254 1103 18 1.4219235525480123 1103 19 1.207784254406409 1103 20 1.2344454019569575 1103 21 1.0475621648219402 1103 22 1.0512840893666535 1103 23 1.3292502182262353 1103 24 1.1277662723973094 1103 25 1.218268909134018 1104 1 1.1956067916796442 1104 2 0.6476841236519881 1104 3 1.2923708265807186 1104 4 1.5224340174492283 1104 5 0.7309580533174369 1104 6 0.19882278811966003 1104 7 0.04260662987574193 1104 8 1.253617190481652 1104 9 -3.661946944068138 1104 10 -1.3275188855805702 1104 11 -0.628481071638241 1104 12 0.7504471086373599 1104 13 1.0231226613883564 1104 14 0.7474002822188694 1104 15 -3.8536958341139176 1104 16 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2.0566842237705956 1106 4 2.3320571478695165 1106 5 1.4550754232396272 1106 6 1.052111798778121 1106 7 0.9641316850525818 1106 8 2.4001816939263527 1106 9 3.263772094387125 1106 10 1.6624412131397612 1106 11 0.8360423418238199 1106 12 2.096396893184945 1106 13 -3.8567972098838084 1106 14 0.326300043391047 1106 15 2.174971477641556 1106 16 1.7902434903518092 1106 17 1.5203937002716543 1106 18 1.2437551602985444 1106 19 1.019068807339007 1106 20 0.7554740224456538 1106 21 0.3365742854012197 1106 22 0.348657044615738 1106 23 0.7177126333638846 1106 24 0.7326022237793381 1106 25 0.6361367188710005 1107 1 1.7732253529988853 1107 2 1.2701147530568768 1107 3 2.035453997334745 1107 4 1.8964006968244682 1107 5 1.5158909359678283 1107 6 0.643214392980088 1107 7 0.3179471763017361 1107 8 1.960198690389781 1107 9 3.1261871424208145 1107 10 1.5503475943779073 1107 11 0.6025660701007993 1107 12 2.203938634911541 1107 13 2.5298139848088113 1107 14 -3.1292778690510197 1107 15 1.042371793850485 1107 16 1.2508511606129495 1107 17 0.9812980055131959 1107 18 1.1180370643880622 1107 19 0.7798166517298455 1107 20 0.8797429363809819 1107 21 0.5270354592218105 1107 22 0.3966075045942006 1107 23 0.944079900790161 1107 24 1.0467332515704229 1107 25 1.139578017101968 1108 1 1.7174555130222835 1108 2 1.2291797375205533 1108 3 1.3982751386098613 1108 4 1.9105218771104795 1108 5 1.242430053080028 1108 6 0.5872995975488955 1108 7 0.35672191698722244 1108 8 -3.762177952312193 1108 9 -5.049111870843753 1108 10 -1.8697950887209416 1108 11 -0.2393803424128862 1108 12 0.6594802497033108 1108 13 1.9769156835240516 1108 14 2.2071724751216593 1108 15 -3.4975985200820965 1108 16 -0.05355885513047909 1108 17 0.5274368918812016 1108 18 0.5582679795143015 1108 19 0.4912691934751976 1108 20 0.4205094744694975 1108 21 0.17025682749259374 1108 22 0.18352800936605362 1108 23 0.4014397750286527 1108 24 0.29126002111685523 1108 25 0.663423226867728 1109 1 0.5126707956409753 1109 2 0.3596146204404924 1109 3 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1110 15 2.1524963799808816 1110 16 1.7603992942509876 1110 17 1.1458171986537948 1110 18 0.8668935348261044 1110 19 0.27291187954899243 1110 20 0.10578553960764836 1110 21 -0.06704917491364881 1110 22 -0.461886864167834 1110 23 0.00188988182533123 1110 24 0.22087895470718522 1110 25 0.40398057055366365 1111 1 1.2950043400254536 1111 2 1.2144932906146124 1111 3 1.3546820068302274 1111 4 2.04999287815734 1111 5 -1.1253936554725357 1111 6 0.17083017728565864 1111 7 -0.30626342977379295 1111 8 1.9630779091384447 1111 9 3.418064784412511 1111 10 1.9514556088797868 1111 11 1.7203737833919917 1111 12 2.8442932970345893 1111 13 3.0050695473288833 1111 14 3.1060529102326946 1111 15 3.4569952172283145 1111 16 2.5272399493554794 1111 17 2.0520627114336065 1111 18 1.4286715938630934 1111 19 1.130377323156715 1111 20 1.1353412934711073 1111 21 0.8984097826767545 1111 22 0.9106978514095623 1111 23 1.1851198012315436 1111 24 1.128384970809197 1111 25 1.1678146049806308 1112 1 1.841043802320666 1112 2 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2.3527871819661925 2204 6 2.118433384912027 2204 7 2.2081076038177088 2204 8 3.7960106521876398 2204 9 -1.9607829057700634 2204 10 0.7500337184055681 2204 11 1.9024625373659632 2204 12 3.599479458915816 2204 13 3.889182858650255 2204 14 4.108252425840458 2204 15 4.463829452847737 2204 16 4.2144768834166655 2204 17 3.2905405140664925 2204 18 3.087326790969108 2204 19 2.53620096071279 2204 20 2.4756490253274386 2204 21 2.088719275812739 2204 22 2.0614155930349476 2204 23 2.46642547144954 2204 24 2.3595968898591693 2204 25 2.418877557417744 2205 1 -2.922019379864153 2205 2 -3.135530180288761 2205 3 -0.059330722735115415 2205 4 -1.602061678789255 2205 5 -2.401060212511105 2205 6 -0.8220725620173432 2205 7 -0.5257972917320544 2205 8 -4.201507180160526 2205 9 0.9265390658166055 2205 10 0.5301203372836909 2205 11 0.31333653441382836 2205 12 2.0062513136554188 2205 13 -3.843796181672765 2205 14 -0.050957056067005624 2205 15 2.131834015428531 2205 16 1.7677347082664703 2205 17 1.667807974166987 2205 18 0.49695876458795496 2205 19 0.6436020462214174 2205 20 0.49177966985296706 2205 21 0.4220438395171051 2205 22 0.5856896293021769 2205 23 0.6718120940597032 2205 24 0.3105759167310289 2205 25 0.8206191727622993 2206 1 -6.327330764243261 2206 2 -4.975718150220313 2206 3 -2.0792210389372805 2206 4 -0.8447937777001471 2206 5 -0.6623406769698548 2206 6 -1.0883370918208257 2206 7 -1.5483679943112776 2206 8 -2.617997947816196 2206 9 -5.327518572963562 2206 10 -2.3522516174360995 2206 11 -1.7019662939762732 2206 12 -6.962382979415629 2206 13 -5.837271751374125 2206 14 -7.022511037794025 2206 15 -2.12123646558447 2206 16 -5.622233103209815 2206 17 -2.6493980999934053 2206 18 -1.8794084496369017 2206 19 -1.8891540134040135 2206 20 -1.8213324415789707 2206 21 -1.7343196481629697 2206 22 -1.7238802560554625 2206 23 -1.352316010131478 2206 24 -1.3238399975878536 2206 25 -1.5484094741092065 2207 1 -6.238203296249473 2207 2 -4.637825005231331 2207 3 -0.6550313487572588 2207 4 0.2064156258430927 2207 5 0.5850289704062668 2207 6 0.14850898597287454 2207 7 0.1854138893301318 2207 8 1.5721215306563572 2207 9 -1.5332253995466019 2207 10 -0.5194907120229999 2207 11 -0.19212796460576342 2207 12 -3.8817270488086812 2207 13 -4.276057588843461 2207 14 -1.0352446127651689 2207 15 -4.482661858785686 2207 16 -0.8953748715559833 2207 17 -0.001181011000340959 2207 18 0.08936503605600166 2207 19 -0.06977782759299345 2207 20 0.01288512781093587 2207 21 -0.16773853531559269 2207 22 -0.14025800990335924 2207 23 -0.3148018748592847 2207 24 -0.39419960251093045 2207 25 0.13114748125437004 2208 1 -3.660774714816153 2208 2 -4.002075552345371 2208 3 -1.3379739535196187 2208 4 -0.2721531201750089 2208 5 -2.7921894780783845 2208 6 -1.1114577001050634 2208 7 -0.860209621529755 2208 8 -4.964334710426645 2208 9 -7.678209466981652 2208 10 -3.7218524589975033 2208 11 -1.5886637415574896 2208 12 -0.062131977091809176 2208 13 0.8258055446068937 2208 14 0.8806384144437445 2208 15 1.273928444529659 2208 16 -4.501116536146821 2208 17 -6.561884025367611 2208 18 -2.8555717008491506 2208 19 -2.203117522556692 2208 20 -1.3436521361027403 2208 21 -1.2839962238299236 2208 22 -0.9417594763697963 2208 23 -0.765080904264785 2208 24 -0.7830820394158664 2208 25 -0.5496079286069424 2209 1 0.7291188235337636 2209 2 0.6402941653026737 2209 3 -2.175317000353388 2209 4 0.12443610168069341 2209 5 0.4315888558144394 2209 6 0.15755958770417067 2209 7 0.25656266413317397 2209 8 1.5666275810883512 2209 9 -3.3606229479107306 2209 10 -0.4288618231249117 2209 11 0.20567707197005802 2209 12 -3.695069213882185 2209 13 0.20864183529948122 2209 14 1.384090936782961 2209 15 2.1465875398970056 2209 16 -2.649866680806955 2209 17 -0.1373700875177093 2209 18 0.4733387395496429 2209 19 0.32078738997830314 2209 20 0.4308512056489242 2209 21 0.4287481984446977 2209 22 0.27275611122119514 2209 23 0.8558795076944428 2209 24 0.38419205480651186 2209 25 0.5226849078232796 2210 1 0.1374134715291575 2210 2 -2.220601236786802 2210 3 -0.4650721857576876 2210 4 -2.4502985226505274 2210 5 -2.7599945966726374 2210 6 -1.1872596828008852 2210 7 -0.4354255295713152 2210 8 -4.444841290365851 2210 9 -4.038585056214629 2210 10 -1.6015713228081516 2210 11 -0.6931490688833648 2210 12 0.9555351414221853 2210 13 -6.412330942839205 2210 14 -1.2731572485068838 2210 15 -4.667845221122231 2210 16 -1.1887026226266144 2210 17 -0.483191023219824 2210 18 0.005879559445602922 2210 19 -0.29559408684031396 2210 20 -0.18869388766175277 2210 21 -0.5030437502549816 2210 22 -0.2792333398440938 2210 23 -0.012432259751843588 2210 24 -0.07189681158600791 2210 25 0.01300651814782583 2211 1 0.4477683138686466 2211 2 0.29640124541504065 2211 3 -3.08742570093532 2211 4 -2.378201922702207 2211 5 -0.5048510661641465 2211 6 -0.5703464637973852 2211 7 -0.6420940731223723 2211 8 -4.156456669609909 2211 9 0.7467438455366988 2211 10 0.22670420737832941 2211 11 -5.5078287509871835 2211 12 -0.27255278927719573 2211 13 0.8792510527692414 2211 14 1.3851873038806524 2211 15 -3.539199473266084 2211 16 -0.5889737923925864 2211 17 -5.341871635760167 2211 18 -1.700532802729036 2211 19 -0.9058995164177901 2211 20 -0.4558881723264072 2211 21 -0.4553324150741176 2211 22 -0.2693756939641432 2211 23 -0.12401036816370947 2211 24 -0.05818754987316055 2211 25 0.13619084376147228 2212 1 2.3099434821791576 2212 2 1.4490678688267131 2212 3 2.3790339686403996 2212 4 2.707262737810339 2212 5 -0.3744181369515722 2212 6 0.8595498840705673 2212 7 1.292146424021702 2212 8 -0.9801866104978849 2212 9 3.0099881017905723 2212 10 2.491336384768946 2212 11 2.1406968335586214 2212 12 3.6213847097628893 2212 13 3.6623147162855005 2212 14 3.908994320154246 2212 15 4.299687268545167 2212 16 3.5482075428891022 2212 17 2.9597542191920425 2212 18 2.61005623940164 2212 19 2.1956773162070107 2212 20 2.0196340455389716 2212 21 1.8848856715944748 2212 22 1.8316269129328973 2212 23 2.028968756913123 2212 24 2.0020798224141174 2212 25 2.3901202309228253 2213 1 2.4095588496093825 2213 2 1.9954967786165767 2213 3 2.3974254933194388 2213 4 2.6068851487701963 2213 5 1.4317051415725501 2213 6 -0.3328942910441981 2213 7 0.8412851792800424 2213 8 -5.546173726527261 2213 9 0.8054812874600827 2213 10 0.968910293218368 2213 11 1.0171755093502277 2213 12 2.545878951237839 2213 13 -1.8655823670933387 2213 14 0.9958375239445552 2213 15 -3.1771858035342397 2213 16 0.23332427517126675 2213 17 1.195963277771039 2213 18 1.389245825982652 2213 19 1.1636252226341846 2213 20 1.4531018575394086 2213 21 1.303027859461647 2213 22 1.3539236662484235 2213 23 1.63535382512676 2213 24 1.67973274044191 2213 25 1.9007379122714738 2214 1 2.130648314451008 2214 2 1.5416058548136427 2214 3 1.3374695648221326 2214 4 2.115193723410705 2214 5 1.8175593319169678 2214 6 0.4793918370874408 2214 7 0.6177396624050111 2214 8 1.9278821488495232 2214 9 2.7948888392217057 2214 10 1.7033733126876562 2214 11 1.515422068869917 2214 12 2.5220434333745203 2214 13 0.8905488838331799 2214 14 2.382652207445073 2214 15 3.3816629326914835 2214 16 2.2010991121036163 2214 17 1.5014665020631774 2214 18 1.4041350141034208 2214 19 1.3156147714803552 2214 20 0.9090010150232253 2214 21 0.5425674404272058 2214 22 0.7329555756223414 2214 23 -4.72874643059296 2214 24 -1.8059308954725033 2214 25 -0.44116599517528243 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 420471.0 3 7.0 4 3022.0 5 18167.0 6 24858.0 7 22865.0 8 18874.0 9 15753.0 10 13962.0 11 12688.0 12 11783.0 13 11259.0 14 10520.0 15 10172.0 16 10290.0 17 11042.0 18 13466.0 19 18789.0 20 26721.0 21 35972.0 22 45264.0 23 54543.0 24 63380.0 25 71540.0 26 79858.0 27 86606.0 28 92276.0 29 105026.0 30 112268.0 31 120364.0 32 145434.0 33 175371.0 34 202787.0 35 226780.0 36 241675.0 37 228294.0 38 196142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42508067328092 18.899319271011056 15.462839123432238 21.212760932275785 2 14.304711574533933 22.72223402694255 41.28189773813651 21.691156660387005 3 15.817410881775693 30.2637460885114 31.12119793818416 22.797645091528747 4 9.512613488843401 18.539631056977402 40.69204964276364 31.255705811415556 5 10.573901902499044 40.565801113773716 34.98493388875143 13.875363094975816 6 26.930770680846965 39.53846894533343 20.06588360382499 13.464876769994616 7 26.029469153135643 35.03814430927456 22.278439726363192 16.653946811226607 8 24.370211447298203 34.269509018871794 23.761189252892542 17.599090280937464 9 25.79683824728646 17.49726280496964 23.551982292068967 33.153916655674934 10 14.451884014139853 32.604596062784424 31.721058631027255 21.222461292048468 11 31.289575792261758 26.681356640189 24.092460714096813 17.936606853452428 12 22.022245240125518 29.15024945793968 31.00591816287747 17.821587139057332 13 26.478063334915575 24.981114803010783 27.787075337356747 20.75374652471689 14 21.119928287268618 24.117406543244282 30.172440097560777 24.590225071926326 15 21.78505498305671 30.706634413104396 26.97860178434301 20.529708819495887 16 20.969113947053742 31.188271714811805 27.008030800954323 20.83458353718013 17 20.158575928896795 30.533240123839345 27.87287074844692 21.435313198816935 18 19.79771103523495 29.557019511158167 30.148297549823823 20.49697190378306 19 21.68880306812253 29.158825784785968 29.581394371244045 19.570976775847452 20 22.407350817935185 28.19569260030911 29.441257659504398 19.9556989222513 21 23.42592489001129 27.89744851965727 28.5163415910044 20.160284999327033 22 22.33448515760509 28.19743117872579 28.831262264690842 20.636821398978274 23 21.589520509395193 28.50527907560233 29.07095728593806 20.834243129064415 24 21.22484089023486 28.416897251929985 29.608504027457982 20.749757830377174 25 21.338938520267266 28.107578089320207 29.73020714983432 20.823276240578203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 16010.0 1 16010.0 2 14758.5 3 13507.0 4 13507.0 5 13507.0 6 16810.5 7 20114.0 8 20114.0 9 20114.0 10 20855.5 11 21597.0 12 21597.0 13 21597.0 14 26113.5 15 30630.0 16 30630.0 17 30630.0 18 45796.5 19 60963.0 20 60963.0 21 60963.0 22 87815.5 23 114668.0 24 114668.0 25 114668.0 26 153180.0 27 191692.0 28 191692.0 29 191692.0 30 224780.0 31 257868.0 32 257868.0 33 257868.0 34 282887.5 35 307907.0 36 307907.0 37 307907.0 38 324835.0 39 341763.0 40 341763.0 41 341763.0 42 352115.5 43 362468.0 44 362468.0 45 362468.0 46 358172.0 47 353876.0 48 353876.0 49 353876.0 50 328320.5 51 302765.0 52 302765.0 53 302765.0 54 264984.0 55 227203.0 56 227203.0 57 227203.0 58 189713.5 59 152224.0 60 152224.0 61 152224.0 62 121826.0 63 91428.0 64 91428.0 65 91428.0 66 70009.0 67 48590.0 68 48590.0 69 48590.0 70 36213.0 71 23836.0 72 23836.0 73 23836.0 74 17257.0 75 10678.0 76 10678.0 77 10678.0 78 7793.0 79 4908.0 80 4908.0 81 4908.0 82 3497.5 83 2087.0 84 2087.0 85 2087.0 86 1522.0 87 957.0 88 957.0 89 957.0 90 710.5 91 464.0 92 464.0 93 464.0 94 266.5 95 69.0 96 69.0 97 69.0 98 43.0 99 17.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24297828913943162 2 0.25325449947587947 3 0.25981234423006 4 0.32251750927647704 5 0.269040651538778 6 0.2989903961377675 7 0.29855095293259043 8 0.2977058698457115 9 0.30767785027088296 10 0.30068056231152535 11 0.3003425290767738 12 0.30064675898805016 13 0.30047774237067437 14 0.2955424571433014 15 0.2955762604667766 16 0.29645514687713065 17 0.2927705846183385 18 0.2956438671137269 19 0.28638175648153374 20 0.33221906311384725 21 0.2930072078826646 22 0.29628613025975487 23 0.3011538088401775 24 0.3011876121636527 25 0.30010590581244767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2958289.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.15156584053562 #Duplication Level Percentage of deduplicated Percentage of total 1 94.83947424720442 77.91211312900998 2 3.0824742153122324 5.064601669019525 3 0.8840551921799417 2.1787955498111353 4 0.3805329348748418 1.2504550581545129 5 0.20241753789114456 0.8314458845671737 6 0.12969596939411662 0.639283618135972 7 0.08150647532041079 0.46871192016003027 8 0.057775628013266675 0.3797086646968137 9 0.04002068660863651 0.295898586382156 >10 0.25454535823666496 3.9704501394904876 >50 0.027746227686956084 1.5903320851762028 >100 0.017731123890480652 2.679378542968631 >500 0.0012551301005922866 0.6751768455925239 >1k 5.263448800975898E-4 0.7149909579440614 >5k 2.429284061988876E-4 1.3486573488908569 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9743 0.3293457806184588 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 7761 0.26234759349069686 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 6439 0.2176595998565387 No Hit TATCAACGCAGAGTACTTTTTTTTT 6220 0.21025667201547923 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5210 0.1761153153055702 No Hit GGTATCAACGCAGAGTACTTTTTTT 5174 0.17489839566046456 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.380332347515743E-5 0.0 0.0 0.0 0.0 6 3.380332347515743E-5 0.0 0.0 0.0 0.0 7 3.380332347515743E-5 3.380332347515743E-5 0.0 0.0 0.0 8 3.380332347515743E-5 3.380332347515743E-5 0.0 0.0 0.0 9 3.380332347515743E-5 3.380332347515743E-5 0.0 0.0 0.0 10 3.380332347515743E-5 3.380332347515743E-5 0.0 0.0 0.0 11 3.380332347515743E-5 3.380332347515743E-5 0.0 3.380332347515743E-5 0.0 12 3.380332347515743E-5 3.380332347515743E-5 0.0 6.760664695031486E-5 0.0 13 3.380332347515743E-5 3.380332347515743E-5 0.0 6.760664695031486E-5 3.380332347515743E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 330 0.0 13.82229 2 GGTATCA 3570 0.0 13.441687 1 TATCACG 150 0.0 13.303954 2 ATAACGC 365 0.0 12.757866 3 GCGCCGA 95 1.0398471E-6 12.000921 19 CGACCGT 65 8.022003E-4 11.693205 19 TAACGCA 425 0.0 11.627578 4 CGGCGGA 75 2.0732135E-4 11.401068 18 CGTCGCC 60 0.0058714533 11.084936 14 GTATCAA 8885 0.0 11.058436 1 ACATGGT 1325 0.0 11.045355 3 AACGGAG 285 0.0 10.997673 5 CTCAACG 345 0.0 10.74287 4 CGTCTCG 115 8.8122397E-7 10.740319 17 TGAATCG 80 3.780219E-4 10.68524 5 CATGGTA 1010 0.0 10.632415 4 ATCACGC 180 3.45608E-11 10.559231 3 TCACGCA 200 1.8189894E-12 10.45364 4 TACATGT 1905 0.0 10.425672 2 CAACGGA 290 0.0 10.15871 4 >>END_MODULE