FastQCFastQC Report
Thu 26 May 2016
SRR1364235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958289
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT309521.0462804682030729No Hit
TATCAACGCAGAGTACTTTTTTTTT242480.8196629876256174No Hit
GGTATCAACGCAGAGTACTTTTTTT200120.6764721093848505No Hit
GTACTTTTTTTTTTTTTTTTTTTTT180520.6102175953735419No Hit
ACGCAGAGTACTTTTTTTTTTTTTT96080.3247823319493126No Hit
GAGTACTTTTTTTTTTTTTTTTTTT94820.3205231131914428No Hit
GTGGTATCAACGCAGAGTACATGGG44380.15001914958274867No Hit
GTGGTATCAACGCAGAGTACTTTTT39770.1344358174607011No Hit
ATCAACGCAGAGTACTTTTTTTTTT37530.12686387300226584No Hit
GCAGAGTACTTTTTTTTTTTTTTTT34780.11756795904659753No Hit
GGTATCAACGCAGAGTACATGGGAT30290.10239026680625186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2950.015.423862
ATAACGC4650.014.8793873
TATAACG4650.014.2698362
ATCACGC3500.013.539953
TAACGCA5350.013.4649364
GGTATCA63150.013.4457071
TCACGCA3650.012.2053294
TAGCGAG550.003121437712.0638865
ACGGTCC658.178095E-411.6663728
AGGACCG1850.011.2720475
GACCGCT600.00597573211.0587487
AACGCCG1553.255991E-1011.0076015
GTATCAC5200.010.934451
TGGTATC18850.010.8621612
CGGTTTG1855.456968E-1210.84071714
GTATAAC7550.010.5434161
GTGGTAT20450.010.4728321
TTAACGC1106.214954E-610.3402984
ACGCCGA2400.010.2690116
CTGGACG1502.5345798E-810.1105134
Produced by FastQC (version 0.11.5)