FastQCFastQC Report
Thu 26 May 2016
SRR1364234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3249131
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT116150.3574802000904242No Hit
TATCAACGCAGAGTACTTTTTTTTT84600.26037731319543594No Hit
GGTATCAACGCAGAGTACTTTTTTT68530.21091793467237857No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN61890.1904817011071576No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA47490.14616215843559402No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44760.13775991180411007No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33840.10415092527817438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50900.013.515351
CGACCGT852.694269E-713.414947519
TATAACG1102.743036E-912.9568192
TCGACCG905.3954864E-712.66967218
ACATGGT20500.012.2379943
CTCAACG3650.011.9763684
GTGGTAT14500.011.6642951
TACATGT27750.011.4362412
CATGGTA19250.011.3542194
GTATCAA130850.011.1393071
GGCACGC600.00588923111.0804888
ACCGATT600.00588923111.0804888
CATCGCG700.001489208510.85971818
AACGGAG2600.010.593275
GTTCGAC1106.0323837E-610.36625616
AGTGACG2500.010.26243419
TAACGCT951.6398606E-410.0031454
ACATGTG24350.09.9517953
CGGGATT1252.5618956E-69.8831074
TCGAACT2500.09.88234419
Produced by FastQC (version 0.11.5)