##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364234_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3249131 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.149674482192314 30.0 25.0 31.0 7.0 34.0 2 25.81611298528745 30.0 25.0 31.0 10.0 34.0 3 25.399656092659853 30.0 25.0 31.0 7.0 34.0 4 28.462014304747946 33.0 26.0 35.0 7.0 37.0 5 29.080942873648368 35.0 28.0 35.0 10.0 37.0 6 29.64905970242505 35.0 30.0 36.0 8.0 37.0 7 29.57934506180268 35.0 30.0 37.0 2.0 37.0 8 27.90718564440769 33.0 25.0 35.0 2.0 37.0 9 27.68352491789343 33.0 18.0 37.0 2.0 39.0 10 29.439725575853974 34.0 27.0 38.0 2.0 39.0 11 29.88586517441125 35.0 28.0 39.0 2.0 39.0 12 28.478829877896583 34.0 23.0 39.0 2.0 39.0 13 28.238188918821677 34.0 23.0 38.0 2.0 39.0 14 28.755335503554644 34.0 23.0 39.0 2.0 41.0 15 28.322449294903777 34.0 19.0 39.0 2.0 40.0 16 29.130436722926838 34.0 25.0 39.0 2.0 41.0 17 29.693918158424513 36.0 27.0 39.0 2.0 41.0 18 30.261771224367376 36.0 27.0 40.0 2.0 41.0 19 30.927371657221578 37.0 30.0 40.0 2.0 41.0 20 30.956941717646966 37.0 30.0 40.0 2.0 41.0 21 31.130767272849262 37.0 30.0 40.0 2.0 41.0 22 31.03653284524385 37.0 30.0 40.0 2.0 41.0 23 30.683784987432023 37.0 30.0 40.0 2.0 41.0 24 30.666090717795004 37.0 30.0 40.0 2.0 41.0 25 30.388353685954797 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.33298549432375424 1101 2 -0.4894353586651441 1101 3 -2.883558794373517 1101 4 -2.9054875302929837 1101 5 -3.4050720946539137 1101 6 -1.6158930616506204 1101 7 -1.1333183718812307 1101 8 -4.726765567174006 1101 9 -6.076649209541294 1101 10 -6.027245204412157 1101 11 -2.9935890591859255 1101 12 -0.3646847378258613 1101 13 0.08635815408504044 1101 14 -7.499068222716826 1101 15 -9.570531318602669 1101 16 -4.273781337860708 1101 17 -2.0389975994336 1101 18 -6.676960396445107 1101 19 -3.1579815341014097 1101 20 -2.085107710287833 1101 21 -2.00767208263904 1101 22 -1.8808559272183203 1101 23 -6.823888832782615 1101 24 -3.4874467038185486 1101 25 -2.5417546233997754 1102 1 1.8734598896813814 1102 2 1.3478791634663203 1102 3 1.8602795068666396 1102 4 1.9510369808064922 1102 5 1.4020978825889365 1102 6 0.8103882299848202 1102 7 0.5912463902822083 1102 8 2.0557092038210136 1102 9 -3.293611997902687 1102 10 -0.4893056231530295 1102 11 0.18189319796753978 1102 12 -4.084974063836476 1102 13 -6.065706359367898 1102 14 -6.184671777397146 1102 15 -1.0817184667965343 1102 16 0.44436791328499936 1102 17 -4.079706739902299 1102 18 -1.4505970719337427 1102 19 -0.543484062963195 1102 20 -0.1009210124901152 1102 21 0.015014197092124704 1102 22 0.04675661719605273 1102 23 0.24007518343785605 1102 24 0.24887760114788904 1102 25 0.3924941660704704 1103 1 -0.7452119470559033 1103 2 0.9738730295013767 1103 3 1.9563383026736325 1103 4 2.587272750480892 1103 5 2.1798365853920316 1103 6 1.416353460551921 1103 7 1.413175763207942 1103 8 2.8198847741891413 1103 9 3.957862828001197 1103 10 2.314179540317536 1103 11 1.9192957505542836 1103 12 3.2608265893368618 1103 13 3.449407173600889 1103 14 -2.5089570457991464 1103 15 1.8218609942823605 1103 16 2.238762108734136 1103 17 2.2694954129574754 1103 18 1.9265026911292082 1103 19 1.495035620438479 1103 20 1.6052150902533135 1103 21 1.4055049929035306 1103 22 1.4237684960465877 1103 23 1.6678088865505885 1103 24 1.4649478334879795 1103 25 1.6205213682478785 1104 1 1.8003959163239465 1104 2 1.2360551689018529 1104 3 1.8873895774882392 1104 4 2.15419567251692 1104 5 1.3815284557194722 1104 6 0.8259381231216523 1104 7 0.6365840272512067 1104 8 2.0784444875764123 1104 9 -3.295445359740551 1104 10 -0.5107476738011982 1104 11 0.11866003984966866 1104 12 1.703973284563311 1104 13 1.7778222751163248 1104 14 1.5580677281962672 1104 15 -3.543047248702077 1104 16 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2.440793829742095 1106 4 2.7960829540637953 1106 5 1.995394455109988 1106 6 1.3956117156829322 1106 7 1.3281725857286197 1106 8 2.857903406507809 1106 9 3.9610376677009853 1106 10 2.070967155556154 1106 11 1.3158069378643447 1106 12 2.5465945189628627 1106 13 -3.7551426816031253 1106 14 0.8732517380082285 1106 15 2.880730475806857 1106 16 2.427706410743113 1106 17 2.026870159390654 1106 18 1.7491612550107334 1106 19 1.5636967053000248 1106 20 1.2395710638718676 1106 21 0.6634540981713322 1106 22 0.8781380197198736 1106 23 1.237291574752021 1106 24 1.1791847573587546 1106 25 1.2403972890285964 1107 1 2.5782083447784103 1107 2 1.979252452723749 1107 3 2.7644050967736113 1107 4 2.7059452593402717 1107 5 2.3174692151585 1107 6 1.3045521229344956 1107 7 1.0296079514852217 1107 8 2.7903180290656557 1107 9 4.130661386705679 1107 10 2.443322254843906 1107 11 1.5138491467872512 1107 12 3.00834373446488 1107 13 3.304871437233686 1107 14 -2.9239985103290564 1107 15 2.0241202345518055 1107 16 2.0957597466804962 1107 17 1.9585606536750895 1107 18 2.031891671104603 1107 19 1.6756229179482531 1107 20 1.7066118223805127 1107 21 1.335643398840105 1107 22 1.1921844560328658 1107 23 1.7817356102586004 1107 24 1.8051725349661787 1107 25 1.8799046758952684 1108 1 1.4439578164303484 1108 2 0.826933522826991 1108 3 1.1899510501223354 1108 4 1.5779172970492183 1108 5 0.8674190537532915 1108 6 0.04477465980990303 1108 7 -0.22334534348714996 1108 8 -4.364576524605216 1108 9 -5.70035263037996 1108 10 -2.29771073470447 1108 11 -0.7783268109456607 1108 12 0.3049105758400721 1108 13 1.6725386201788979 1108 14 2.007582602554919 1108 15 -3.8412364224190334 1108 16 -0.3320649929131818 1108 17 0.21759423860394023 1108 18 0.16404030298330596 1108 19 -0.04043213623888775 1108 20 -0.11686768825034122 1108 21 -0.3488797343063865 1108 22 -0.33174966858507915 1108 23 -0.024423798474142444 1108 24 -0.1378872431664817 1108 25 0.2584712819227306 1109 1 0.7129659078460691 1109 2 0.530768295791681 1109 3 0.7976941429667406 1109 4 -1.4843909130539856 1109 5 -2.4878677687564164 1109 6 -0.9429261038145782 1109 7 -0.7677090267066156 1109 8 1.39590317866352 1109 9 -3.754286616375925 1109 10 -0.7623494365303003 1109 11 -0.239515124934929 1109 12 -7.061777901098704 1109 13 -1.6373974090658336 1109 14 0.19359835531679792 1109 15 1.703596446609307 1109 16 1.3443664421594939 1109 17 0.8601859348309162 1109 18 0.523893895256446 1109 19 0.014417211816660114 1109 20 -0.015026756254464146 1109 21 -0.09083339640307742 1109 22 -0.22526143337217164 1109 23 0.06251949049536876 1109 24 -0.20679020181914254 1109 25 0.13959762908006823 1110 1 0.31319518693696935 1110 2 0.14851286877594205 1110 3 -3.2193451190316758 1110 4 -2.818194707287528 1110 5 -0.6564206061500002 1110 6 -0.7063659725289 1110 7 -0.771878195878525 1110 8 -4.260807716994812 1110 9 -8.344936589230294 1110 10 -3.3710310192280026 1110 11 -1.2927700981931451 1110 12 1.1003069825920377 1110 13 1.399434002768981 1110 14 1.5515298780134472 1110 15 2.2476338488848135 1110 16 1.6345707575269337 1110 17 1.0719225588317371 1110 18 0.7019177790906035 1110 19 -0.10553043504847537 1110 20 -0.07822001912374077 1110 21 -0.35833862648654957 1110 22 -0.6577506447806734 1110 23 -0.1312744770797707 1110 24 -0.10743370069737068 1110 25 0.12214307823964532 1111 1 1.3240146794534873 1111 2 1.1766187699657813 1111 3 1.3395748540819952 1111 4 2.140256577901919 1111 5 -1.2364353794197918 1111 6 -0.06324165192285491 1111 7 -0.3710117105272275 1111 8 1.8353712601670686 1111 9 3.2736450196328306 1111 10 1.8354877384802393 1111 11 1.4921640725872045 1111 12 2.775678442857078 1111 13 2.999967594545243 1111 14 3.198611644753953 1111 15 3.592386222490603 1111 16 2.6670233241379684 1111 17 2.03318884841741 1111 18 1.3812076383543577 1111 19 0.9837383929213175 1111 20 0.9898049540382665 1111 21 0.7644038172084109 1111 22 0.7514930963507354 1111 23 1.1055953193288701 1111 24 1.0124833726205154 1111 25 1.170723247512619 1112 1 2.0711846850365276 1112 2 1.4155301803891653 1112 3 2.057750331714722 1112 4 2.2592984094648116 1112 5 1.745482069747343 1112 6 0.9462229060857368 1112 7 0.8058051684219834 1112 8 -3.542706513080031 1112 9 1.6628964225242662 1112 10 0.9836335293984142 1112 11 1.1755771601996905 1112 12 2.0780083489378427 1112 13 2.4757289578519917 1112 14 2.8360173965069535 1112 15 3.55101252155405 1112 16 2.3212663500733335 1112 17 1.7876847052461322 1112 18 1.5658529978994515 1112 19 1.2444907176494837 1112 20 1.11084681532623 1112 21 0.9052828474424004 1112 22 0.8365966894164529 1112 23 1.2312752503283555 1112 24 1.2225134620618086 1112 25 1.2252540073261464 1113 1 0.08724556586658849 1113 2 0.26271633912024583 1113 3 -6.354937632817393 1113 4 -3.6995554705627605 1113 5 -0.9417751330511059 1113 6 -0.8583440855362205 1113 7 -0.23754355200090416 1113 8 1.3472747864340313 1113 9 -6.396716831384531 1113 10 -2.0833388991029373 1113 11 -0.5838466240060782 1113 12 -4.704638303198369 1113 13 -0.07072647575441238 1113 14 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-1.2366599130739928 2204 4 1.7948055505228382 2204 5 1.9463277851010474 2204 6 1.6553654533275264 2204 7 1.6963055395783684 2204 8 3.381939154837294 2204 9 -2.280006167350571 2204 10 0.4963098459014752 2204 11 1.6149476798898057 2204 12 3.4002135918747953 2204 13 3.538901657273641 2204 14 3.800156376836007 2204 15 4.224850094886996 2204 16 3.74819423368751 2204 17 2.704874756372263 2204 18 2.615443876338098 2204 19 1.9387732332103838 2204 20 1.901247354254945 2204 21 1.6132186485237057 2204 22 1.5741972190691236 2204 23 2.066675774332751 2204 24 1.9701673587905582 2204 25 2.0437254147378425 2205 1 -3.040188006478772 2205 2 -3.4458859341680004 2205 3 -0.08685887711977358 2205 4 -1.9186904969322462 2205 5 -2.4446864209186714 2205 6 -0.7826671847529525 2205 7 -0.5063499368308406 2205 8 -4.052512941265036 2205 9 1.283692898379666 2205 10 0.7275108225982052 2205 11 0.42171806762315356 2205 12 2.1768525086197705 2205 13 -4.334819726441417 2205 14 0.02275986706728972 2205 15 2.3382444798012294 2205 16 1.9297908374266903 2205 17 1.868057050303456 2205 18 0.5767061529829327 2205 19 0.7082327671800037 2205 20 0.641959772206107 2205 21 0.619506429558502 2205 22 0.7287973668622243 2205 23 0.9332467027468923 2205 24 0.5664762349669417 2205 25 1.0373893116718804 2206 1 -7.127148219263901 2206 2 -5.474507273838171 2206 3 -2.2801279885276173 2206 4 -1.0407038377009492 2206 5 -0.9788015504417231 2206 6 -1.4622096399298457 2206 7 -1.8301129579138014 2206 8 -3.2587116813102064 2206 9 -5.844508961597022 2206 10 -2.7545470848549307 2206 11 -1.9817835097168306 2206 12 -8.037960312264193 2206 13 -6.822787806035979 2206 14 -7.963034607114373 2206 15 -2.6343839265054463 2206 16 -6.453318851687314 2206 17 -3.084013993261994 2206 18 -2.3064337612914407 2206 19 -2.4193662071895368 2206 20 -2.400018482213895 2206 21 -2.294798860257952 2206 22 -2.4243587929116295 2206 23 -1.9051941534740884 2206 24 -1.8913960922781428 2206 25 -2.093011765721787 2207 1 -7.238492527755369 2207 2 -5.414469710763743 2207 3 -1.2464333452048777 2207 4 -0.17068517778331227 2207 5 0.16950905780473136 2207 6 -0.25258074858273716 2207 7 -0.20232733044786144 2207 8 1.2890761544796447 2207 9 -1.9845916760884208 2207 10 -0.744231198678392 2207 11 -0.5048111876959958 2207 12 -4.3111794062738475 2207 13 -4.666102971122271 2207 14 -1.0753119414433492 2207 15 -4.972260839347705 2207 16 -1.0088727264030979 2207 17 -0.15804002416463092 2207 18 -0.03034171935749086 2207 19 -0.32467946686254834 2207 20 -0.310999004389636 2207 21 -0.41706271937766815 2207 22 -0.3730975799462861 2207 23 -0.3029567546999168 2207 24 -0.5482179139692285 2207 25 -0.1708886138600363 2208 1 -3.4128186098835833 2208 2 -4.036090124638559 2208 3 -1.0315558697013039 2208 4 0.16246556063000384 2208 5 -2.4665714611491367 2208 6 -0.8874381034209975 2208 7 -0.42830336254878176 2208 8 -4.687190245650054 2208 9 -8.069457348888175 2208 10 -3.6835968723474295 2208 11 -1.260189230624718 2208 12 0.4428291340070345 2208 13 1.2769706607116085 2208 14 1.26617862639036 2208 15 1.7228914679059493 2208 16 -4.644588472048852 2208 17 -6.752898442789213 2208 18 -2.505150384511026 2208 19 -2.0243036156161693 2208 20 -1.0713205025658787 2208 21 -0.8718222449919928 2208 22 -0.6033386997753496 2208 23 -0.34261944098748387 2208 24 -0.3482893528168347 2208 25 -0.1357658992077333 2209 1 0.6997300374414479 2209 2 0.671267006699189 2209 3 -2.430476939004169 2209 4 0.07888483211889508 2209 5 0.3291521512359772 2209 6 0.1326700050363847 2209 7 0.019092102409931755 2209 8 1.4777106908286228 2209 9 -3.8057266944031163 2209 10 -0.7903642453151463 2209 11 -0.1150582170345622 2209 12 -4.514291918980216 2209 13 0.04633612593686465 2209 14 1.2616810087126318 2209 15 2.2293984371779345 2209 16 -3.1762037207421905 2209 17 -0.4019095845242653 2209 18 0.14393231977812349 2209 19 -0.010815339571021099 2209 20 0.0648443260811149 2209 21 0.16384070345523938 2209 22 -0.03741141411502724 2209 23 0.39672082118777396 2209 24 -0.07719922465309281 2209 25 0.07780295875028997 2210 1 -0.4098344364444735 2210 2 -3.183033357552734 2210 3 -1.0766743713195979 2210 4 -3.236566941746947 2210 5 -3.590633184115461 2210 6 -1.8214865585155344 2210 7 -1.0973281670112591 2210 8 -5.278283973863125 2210 9 -4.610973530679761 2210 10 -2.13950457191115 2210 11 -1.1284082011646674 2210 12 0.5052476488835644 2210 13 -7.346371926254587 2210 14 -1.8554495735506507 2210 15 -5.497962506897412 2210 16 -1.7250561760419814 2210 17 -1.0733456050881962 2210 18 -0.7678052504218797 2210 19 -1.1590392605799558 2210 20 -0.9620217902851778 2210 21 -1.2558891952149303 2210 22 -1.0837908644336132 2210 23 -0.6997524990322006 2210 24 -0.809366210517009 2210 25 -0.6351224786435061 2211 1 0.19033281268237445 2211 2 0.0690765160767235 2211 3 -3.4224731422329917 2211 4 -2.933822223052328 2211 5 -0.8877383752851031 2211 6 -0.9688026484058696 2211 7 -1.140163756370491 2211 8 -4.583028058846224 2211 9 0.34560199993276086 2211 10 -0.028650290533352774 2211 11 -6.025748305046939 2211 12 -0.42643848039831056 2211 13 0.5746406282818874 2211 14 1.1205441437014088 2211 15 -4.294836610401909 2211 16 -0.9306845238986767 2211 17 -6.236477057572618 2211 18 -2.264235546345173 2211 19 -1.282417268533905 2211 20 -0.8820743223699132 2211 21 -0.8900972986424946 2211 22 -0.5688915255006677 2211 23 -0.45114874880951916 2211 24 -0.4965460373884838 2211 25 -0.2831829754226227 2212 1 2.032053467723024 2212 2 1.2809866200027642 2212 3 2.2390576085225753 2212 4 2.6241240793154788 2212 5 -0.9080442384386274 2212 6 0.6414704721190994 2212 7 1.0938317525003534 2212 8 -1.2778785680187852 2212 9 2.85194533455741 2212 10 2.133131974948647 2212 11 1.9896952303105806 2212 12 3.4687479274097655 2212 13 3.587113094232926 2212 14 3.8590657421421497 2212 15 4.448877763605935 2212 16 3.529398822082232 2212 17 2.766019891118816 2212 18 2.46776173309879 2212 19 2.0178230484103494 2212 20 1.829545605746599 2212 21 1.6375069323776756 2212 22 1.7145393182243573 2212 23 1.8752729580999343 2212 24 1.8771290041917759 2212 25 2.2467312553128167 2213 1 2.6484825760529453 2213 2 2.1932398026245146 2213 3 2.855133948054977 2213 4 3.130420045226529 2213 5 1.9295045452929536 2213 6 -0.10122124313759429 2213 7 1.3689717251429983 2213 8 -6.024912894519343 2213 9 1.014012971730235 2213 10 1.5218024267242427 2213 11 1.6052379500650318 2213 12 3.38594351938589 2213 13 -1.4143070964299511 2213 14 1.7589623544496042 2213 15 -3.13313496560491 2213 16 0.7007458282601533 2213 17 1.7463946427905448 2213 18 2.0097269087111087 2213 19 1.9103350338897656 2213 20 2.095193818867809 2213 21 1.916277233219276 2213 22 2.046252285112036 2213 23 2.2664379501630947 2213 24 2.3969326423228132 2213 25 2.6891195529417544 2214 1 2.747054874128633 2214 2 1.904385734021531 2214 3 1.674269660821313 2214 4 2.309372024822963 2214 5 2.0424098839588467 2214 6 0.765477435727405 2214 7 1.1220308908461405 2214 8 2.400289129830991 2214 9 3.4980715287803967 2214 10 1.8150851302386322 2214 11 2.032326588051294 2214 12 3.2880535865904434 2214 13 1.555070287373141 2214 14 3.3153486827136405 2214 15 4.276308211690015 2214 16 2.954390269615697 2214 17 2.161843898191023 2214 18 1.7283809642564805 2214 19 1.6078024899098509 2214 20 1.2452552106823767 2214 21 1.238680928100905 2214 22 1.2878032761212417 2214 23 -5.4488565094099215 2214 24 -1.396002349747846 2214 25 0.2351246622570926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 281746.0 3 6.0 4 1955.0 5 14192.0 6 22140.0 7 20531.0 8 17396.0 9 13553.0 10 12084.0 11 11325.0 12 10154.0 13 9745.0 14 9118.0 15 8807.0 16 8586.0 17 9444.0 18 11617.0 19 16638.0 20 24363.0 21 33240.0 22 42924.0 23 51943.0 24 61545.0 25 71791.0 26 81524.0 27 89483.0 28 97561.0 29 112742.0 30 122909.0 31 133595.0 32 166954.0 33 209394.0 34 251174.0 35 289079.0 36 320394.0 37 315785.0 38 293694.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06390877260513 19.063476964910073 15.645318094222906 21.227296168261887 2 14.722710290060363 22.135641592592776 41.1444477896285 21.99720032771836 3 15.700421916197332 29.73221262453962 31.51445919795511 23.05290626130794 4 9.324414926324055 17.558908652694537 39.26120943624999 33.85546698473141 5 10.329002189481296 40.98306692124747 34.32734611852199 14.360584770749247 6 27.88248617557026 37.91032018366742 19.86326774958033 14.34392589118199 7 27.00826601337584 35.248790431704556 21.194901344465364 16.548042210454238 8 24.438571236814212 33.65644203961792 23.737088071885594 18.167898651682275 9 25.668602839019954 16.49008429169853 23.849699451983604 33.99161341729791 10 14.951826733000184 32.85962388376946 30.651201600694193 21.537347782536166 11 32.38176879237366 25.679590873842788 23.01059999142151 18.92804034236204 12 21.864414677265014 29.46324342388874 30.261976756995786 18.41036514185046 13 26.6943234648101 24.21425296095812 27.753763568936584 21.337660005295195 14 21.212807541892715 23.85954854050478 29.63893237981744 25.288711537785062 15 21.779803480379513 30.01929155676568 27.345473149189274 20.855431813665533 16 21.842824800285612 30.835580496261205 26.143183389786262 21.178411313666917 17 20.53572475489122 30.325449903511657 27.197308323916953 21.94151701768017 18 20.325975168469256 28.664134986464756 29.844677192260306 21.165212652805682 19 22.176574586317226 28.545977951317646 29.6283257379644 19.64912172440073 20 23.701316040081604 26.935589572559394 29.008878246613435 20.354216140745567 21 24.90426905994531 26.805650609376418 27.95636582209504 20.33371450858323 22 23.705043807544264 27.320917338678573 28.07965771055362 20.894381143223548 23 22.237555336530658 27.717052199173864 28.750494503222523 21.29489796107295 24 21.92807103406606 27.689096971967412 29.062552266781378 21.320279727185145 25 21.800083255185164 27.170937583606197 29.52646067642832 21.502518484780317 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12429.0 1 12429.0 2 11089.5 3 9750.0 4 9750.0 5 9750.0 6 13359.0 7 16968.0 8 16968.0 9 16968.0 10 18385.0 11 19802.0 12 19802.0 13 19802.0 14 24980.5 15 30159.0 16 30159.0 17 30159.0 18 46025.0 19 61891.0 20 61891.0 21 61891.0 22 90779.0 23 119667.0 24 119667.0 25 119667.0 26 160891.5 27 202116.0 28 202116.0 29 202116.0 30 234449.5 31 266783.0 32 266783.0 33 266783.0 34 291787.5 35 316792.0 36 316792.0 37 316792.0 38 337181.0 39 357570.0 40 357570.0 41 357570.0 42 376885.5 43 396201.0 44 396201.0 45 396201.0 46 400311.5 47 404422.0 48 404422.0 49 404422.0 50 376746.0 51 349070.0 52 349070.0 53 349070.0 54 306178.0 55 263286.0 56 263286.0 57 263286.0 58 223144.0 59 183002.0 60 183002.0 61 183002.0 62 148691.0 63 114380.0 64 114380.0 65 114380.0 66 88469.5 67 62559.0 68 62559.0 69 62559.0 70 47369.5 71 32180.0 72 32180.0 73 32180.0 74 23675.5 75 15171.0 76 15171.0 77 15171.0 78 11718.0 79 8265.0 80 8265.0 81 8265.0 82 6092.0 83 3919.0 84 3919.0 85 3919.0 86 2932.0 87 1945.0 88 1945.0 89 1945.0 90 1314.0 91 683.0 92 683.0 93 683.0 94 396.0 95 109.0 96 109.0 97 109.0 98 60.5 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21381101593010562 2 0.2253833409610139 3 0.23058473173288488 4 0.29358619273892 5 0.23772510249663678 6 0.2613314144612821 7 0.2619777411252424 8 0.25933087954902406 9 0.2682871204639025 10 0.26274717762995703 11 0.2606235328769446 12 0.2616084116029794 13 0.2602849808148702 14 0.25708412495525723 15 0.25653013067186275 16 0.2578535614599719 17 0.25622235606997684 18 0.256437798291297 19 0.24994375419150533 20 0.2966947162179672 21 0.25474503798092474 22 0.2574534544775203 23 0.2617622989039223 24 0.26299339731146576 25 0.2613621919214707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3249131.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.15623552930357 #Duplication Level Percentage of deduplicated Percentage of total 1 92.06080409926778 69.18943475901645 2 4.459650526467686 6.7034109069117624 3 1.4002835697824056 3.1572012533514138 4 0.6555715930747527 1.9708117222178745 5 0.36297193989370824 1.3639802302589876 6 0.23756034660707864 1.0712484817214758 7 0.15303622419400134 0.8051138557027768 8 0.11776100022813987 0.7080378775449963 9 0.0797616227524352 0.539512497520333 >10 0.40983195684732193 5.537887033641556 >50 0.03477664525071912 1.8273300116168079 >100 0.024997154955652474 3.6036606564498452 >500 0.0016180111819232195 0.8084817295196827 >1k 0.00121350837913521 1.7071021412354719 >5k 1.2135083791352099E-4 0.6536803063210357 >10k+ 4.045027930450699E-5 0.35310653696952976 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 11615 0.3574802000904242 No Hit TATCAACGCAGAGTACTTTTTTTTT 8460 0.26037731319543594 No Hit GGTATCAACGCAGAGTACTTTTTTT 6853 0.21091793467237857 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 6189 0.1904817011071576 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 4749 0.14616215843559402 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4476 0.13775991180411007 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3384 0.10415092527817438 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 3.077746018858581E-5 0.0 12 3.077746018858581E-5 0.0 0.0 3.077746018858581E-5 0.0 13 3.077746018858581E-5 0.0 0.0 3.077746018858581E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5090 0.0 13.51535 1 CGACCGT 85 2.694269E-7 13.4149475 19 TATAACG 110 2.743036E-9 12.956819 2 TCGACCG 90 5.3954864E-7 12.669672 18 ACATGGT 2050 0.0 12.237994 3 CTCAACG 365 0.0 11.976368 4 GTGGTAT 1450 0.0 11.664295 1 TACATGT 2775 0.0 11.436241 2 CATGGTA 1925 0.0 11.354219 4 GTATCAA 13085 0.0 11.139307 1 GGCACGC 60 0.005889231 11.080488 8 ACCGATT 60 0.005889231 11.080488 8 CATCGCG 70 0.0014892085 10.859718 18 AACGGAG 260 0.0 10.59327 5 GTTCGAC 110 6.0323837E-6 10.366256 16 AGTGACG 250 0.0 10.262434 19 TAACGCT 95 1.6398606E-4 10.003145 4 ACATGTG 2435 0.0 9.951795 3 CGGGATT 125 2.5618956E-6 9.883107 4 TCGAACT 250 0.0 9.882344 19 >>END_MODULE