FastQCFastQC Report
Thu 26 May 2016
SRR1364234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3249131
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT375021.154216311992345No Hit
TATCAACGCAGAGTACTTTTTTTTT311420.9584716651929392No Hit
GGTATCAACGCAGAGTACTTTTTTT261270.8041227023471814No Hit
ACGCAGAGTACTTTTTTTTTTTTTT131430.40450815925858324No Hit
GTACTTTTTTTTTTTTTTTTTTTTT109520.3370747439853918No Hit
GAGTACTTTTTTTTTTTTTTTTTTT85560.2633319493735402No Hit
GTGGTATCAACGCAGAGTACATGGG76260.2347089113981554No Hit
GGTATCAACGCAGAGTACATGGGAT58680.18060213638662154No Hit
GGTATCAACGCAGAGTACATGGGAG47940.14754714414408038No Hit
ATCAACGCAGAGTACTTTTTTTTTT47510.14622371335597117No Hit
GCAGAGTACTTTTTTTTTTTTTTTT47250.14542349939106794No Hit
GTGGTATCAACGCAGAGTACTTTTT39380.12120163822265091No Hit
GGTATCAACGCAGAGTACATGGGAA39340.12107852838189657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG456.8970525E-414.7401813
GGTATCA81650.013.9428371
GTGGTAT21600.013.5602921
CGCGCCA1001.0448275E-813.26657110
TGGTATC21750.012.9455852
CCGAGTG1650.012.6348299
TGCACCG854.042933E-612.2677475
ATAACGC2050.012.0227523
TCTCGTC1303.3938704E-810.93398712
GTCTAGC1502.215529E-910.742911
ACCGCTC803.8497522E-410.66424758
TATAACG2150.010.582722
GGACCGA2150.010.5815796
CGAGTGG1909.094947E-1210.47360810
AGGACCG2450.010.4469315
ACCGAGT2200.010.3410888
AATTTCG750.002540459210.18451115
GTATCAA171950.09.9338351
CACGCCC3650.09.67877416
ACGCCCC3700.09.54797917
Produced by FastQC (version 0.11.5)