FastQCFastQC Report
Thu 26 May 2016
SRR1364233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918566
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA175220.9132862773550663No Hit
GTATCAACGCAGAGTACTTTTTTTT157300.8198831835860741No Hit
TATCAACGCAGAGTACTTTTTTTTT117500.6124365802375316No Hit
GTACTTTTTTTTTTTTTTTTTTTTT104270.5434788274158929No Hit
GGTATCAACGCAGAGTACTTTTTTT92930.4843721821402026No Hit
GAGTACTTTTTTTTTTTTTTTTTTT52410.273172775917013No Hit
AAACAAAAAAAAAAAAAAAAAAAAA51820.2700975624502884No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA48770.2542002724951865No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40470.21093879491245024No Hit
GAACAAAAAAAAAAAAAAAAAAAAA37260.19420754876298235No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN36680.1911844575584056No Hit
GTATCAACTCAGAGTACTTTTTTTT27490.14328409864450845No Hit
TATCAACTCAGAGTACTTTTTTTTT22280.11612840006546557No Hit
GGTATCAACGCAGAGTACATGGGAT19910.10377542393641917No Hit
GCAGAGTACTTTTTTTTTTTTTTTT19540.1018469002369478No Hit
GTATCAACGCAGATTACTTTTTTTT19410.10116931082902543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1650.016.6981182
TCACGCA1750.015.2030324
ATAACGC2550.014.532313
AGAGCCG400.00528552614.2476295
ATCACGC1750.014.1171013
CTCGCAG551.9567768E-413.821318
TATAACG2800.013.5723622
TAACGCA2700.013.3730374
TCGGGAT1301.4551915E-1113.156473
TCTCGCA655.444911E-513.15612717
GGTATCA50100.012.8950991
ACATGGT12100.012.8000733
AACTCAT4400.012.7368496
CGGTTTG751.4767702E-512.6685314
GTATCAC2750.012.437221
TACAACG853.941628E-612.2949632
ACTCATA3950.012.2634627
TACATGT12850.011.90354252
CGGGATT1406.7848305E-1011.5380164
TGCGCGC7500.011.52896619
Produced by FastQC (version 0.11.5)