FastQCFastQC Report
Thu 26 May 2016
SRR1364233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918566
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT506602.640513800411349No Hit
TATCAACGCAGAGTACTTTTTTTTT450452.347847298451031No Hit
GTACTTTTTTTTTTTTTTTTTTTTT366261.9090299734280707No Hit
GGTATCAACGCAGAGTACTTTTTTT345581.8012411353062652No Hit
GAGTACTTTTTTTTTTTTTTTTTTT194201.012214330911733No Hit
ACGCAGAGTACTTTTTTTTTTTTTT177520.9252743976490775No Hit
GCAGAGTACTTTTTTTTTTTTTTTT72270.3766875885426928No Hit
ATCAACGCAGAGTACTTTTTTTTTT70490.3674098258803711No Hit
GTGGTATCAACGCAGAGTACTTTTT58360.30418552189499865No Hit
GTGGTATCAACGCAGAGTACATGGG38480.2005664647450231No Hit
GGTATCAACGCAGAGTACATGGGAT36130.18831773314027248No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA31670.16507120422232022No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT31670.16507120422232022No Hit
GTATAACGCAGAGTACTTTTTTTTT31250.16288206921210946No Hit
GGTATCAACGCAGAGTACATGGGAG26300.13708154944891132No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT26140.1362475932545453No Hit
CAACGCAGAGTACTTTTTTTTTTTT26110.1360912264681017No Hit
GGTATCAACGCAGAGTACATGGGAA24610.12827288714592044No Hit
GGTCGGGAGTGGGTAATTTGCGCGC23890.12452008427127344No Hit
TCAACGCAGAGTACTTTTTTTTTTT21650.11284469755014945No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT21300.11102041837497381No Hit
AACGCAGAGTACTTTTTTTTTTTTT20840.10862279431617156No Hit
CTACCTCCCCGGGTCGGGAGTGGGT19810.10325420131494042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC350.002197845416.2551578
TATAACG4750.015.3727082
TATCACG3100.015.2954252
ATAACGC4800.015.21019653
GGTATCG508.868179E-515.16910651
AGGACCG1101.2732926E-1114.6535065
TAACGCA5100.014.3154774
GTACGAG400.005351437314.2210381
GTCGGTT602.6270129E-514.21621812
TACAACG1750.014.08927252
CGAGTGG759.935593E-713.90066410
GCGTATC551.9946523E-413.7900961
CGTGCGC551.9999521E-413.78578410
GGTATCA87750.013.6349091
TTCAACG1750.013.5473782
GGTCGGT707.4449326E-613.53925611
AATATCG500.001521741713.2743535
GCGCTTA655.580669E-513.12163713
CCGAGTG1102.8448994E-912.9241729
AAACGCA3600.012.9056215
Produced by FastQC (version 0.11.5)