FastQCFastQC Report
Thu 26 May 2016
SRR1364232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434047
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN24060.16777692781338407No Hit
GTATCAACGCAGAGTACTTTTTTTT15430.10759758920035396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG2800.012.55636718
TGCGCGC2950.012.24001319
TATCACG701.0888734E-412.21657852
TTTGCGC2900.012.12381417
CGGTTTG907.437924E-611.61410214
ATCAGTA1251.8257197E-811.4021412
TAAGGCT1950.011.2084074
ATTTGCG3000.011.08657716
ACGGAGA1201.2787677E-711.0807656
GACATAT1900.011.0020651
GGACCGA951.3633728E-510.997456
CGAGCAA700.001487640210.86032116
AATAACC1408.3200575E-910.8591812
CGACCGT1158.787538E-710.74152219
CCCTTAT1158.791485E-710.7411461
AGGACCG1158.8391243E-710.7366415
ACATGGT7100.010.7073583
GGTATCA16350.010.5769081
ATCGTCT1451.4364559E-810.4854615
AATTTGC3300.010.36630715
Produced by FastQC (version 0.11.5)