##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364232_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1434047 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.683540358161203 30.0 25.0 31.0 10.0 34.0 2 26.3723824951344 30.0 26.0 31.0 10.0 34.0 3 25.913417063736404 30.0 25.0 31.0 10.0 34.0 4 29.109635876648394 35.0 28.0 35.0 10.0 37.0 5 29.69675540620356 35.0 28.0 35.0 10.0 37.0 6 30.49847947800874 35.0 30.0 37.0 15.0 37.0 7 30.513085693844065 35.0 31.0 37.0 11.0 37.0 8 28.752718007150392 35.0 27.0 36.0 10.0 37.0 9 28.66523133481678 34.0 23.0 38.0 8.0 39.0 10 30.445734344829702 35.0 28.0 39.0 10.0 39.0 11 31.013469572475657 35.0 30.0 39.0 10.0 39.0 12 29.554538310111173 35.0 27.0 39.0 8.0 39.0 13 29.33724487412198 35.0 27.0 39.0 8.0 39.0 14 29.901718702385626 35.0 27.0 39.0 7.0 41.0 15 29.445543277172924 34.0 25.0 39.0 2.0 41.0 16 30.299181268117433 35.0 27.0 39.0 2.0 41.0 17 30.858222917379976 36.0 28.0 40.0 2.0 41.0 18 31.40985128102496 36.0 30.0 40.0 2.0 41.0 19 32.0769633073393 37.0 31.0 40.0 2.0 41.0 20 32.101229597077364 37.0 31.0 40.0 2.0 41.0 21 32.28436167015447 37.0 31.0 40.0 2.0 41.0 22 32.186108962955885 38.0 31.0 40.0 2.0 41.0 23 31.78148414940375 37.0 31.0 40.0 2.0 41.0 24 31.772938404389816 37.0 31.0 40.0 2.0 41.0 25 31.485906668330955 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.23360541481931918 1101 2 -0.42158670100322126 1101 3 -2.4956775763162504 1101 4 -2.8112966296367574 1101 5 -3.2518296970508835 1101 6 -1.4901674680633832 1101 7 -1.1526440499197328 1101 8 -4.8699699113748665 1101 9 -6.357352210901791 1101 10 -6.523874756241465 1101 11 -3.245836109679864 1101 12 -0.4094837963618012 1101 13 0.1449500837998592 1101 14 -7.9241018286616445 1101 15 -10.08603612786888 1101 16 -4.665937319274359 1101 17 -2.292102467772324 1101 18 -7.220372318742584 1101 19 -3.4446938158730553 1101 20 -2.2916574447258924 1101 21 -2.124392180333288 1101 22 -2.1034640610670365 1101 23 -7.279202265864107 1101 24 -3.6499354346429094 1101 25 -2.5297600928521575 1102 1 2.006191502335138 1102 2 1.6315223145528464 1102 3 1.981692685753167 1102 4 2.0733092757022185 1102 5 1.6548829854725255 1102 6 0.9420158498258289 1102 7 0.7977941000447224 1102 8 2.263398706057 1102 9 -3.441111209958734 1102 10 -0.3838808459720937 1102 11 0.2513486353983865 1102 12 -4.246581890258529 1102 13 -6.2704016347760465 1102 14 -6.142626742341619 1102 15 -0.8447251301904792 1102 16 0.7227750514360878 1102 17 -4.244337058966188 1102 18 -1.4475153148783306 1102 19 -0.5559805659907191 1102 20 -0.03319101942121705 1102 21 0.029908347580125394 1102 22 0.113700480641171 1102 23 0.15624409849637644 1102 24 0.32811193631077273 1102 25 0.6024399586087057 1103 1 -0.735409488635355 1103 2 0.9814657049501925 1103 3 2.0645877630930656 1103 4 2.773057225024157 1103 5 2.346626363535858 1103 6 1.6261435617975906 1103 7 1.6462630146304633 1103 8 3.170740427112854 1103 9 4.456519537273266 1103 10 2.6982616497073124 1103 11 2.230195646656277 1103 12 3.5766641046526537 1103 13 3.8915471223945772 1103 14 -2.322202522933008 1103 15 2.191637060319348 1103 16 2.538480328595938 1103 17 2.678298549580404 1103 18 2.466774618939926 1103 19 1.942468829568618 1103 20 2.040950298329946 1103 21 1.7266449375073663 1103 22 1.7467163752956694 1103 23 2.035961749804237 1103 24 1.8556069119687955 1103 25 2.0040385417900026 1104 1 1.6907441896191635 1104 2 1.1529559793816446 1104 3 1.6702950809852588 1104 4 2.0319307044858697 1104 5 1.235103432685193 1104 6 0.6751953503451738 1104 7 0.4175505506238828 1104 8 2.0947303027834003 1104 9 -3.524258088462343 1104 10 -0.7845874339821073 1104 11 -0.185525187131784 1104 12 1.4579655762902597 1104 13 1.6228851121359256 1104 14 1.3861626413145096 1104 15 -4.030753994004478 1104 16 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2.341634454489732 1106 4 2.5852785194615926 1106 5 1.7409447674301823 1106 6 1.3368969856095099 1106 7 1.069454027517505 1106 8 2.8005811977637514 1106 9 3.983774829686574 1106 10 1.830497379847003 1106 11 0.9892373075389216 1106 12 2.3597490088045916 1106 13 -4.301541536726219 1106 14 0.4922356183965526 1106 15 2.5133790225932024 1106 16 2.1158560239201094 1106 17 1.5718329870411978 1106 18 1.3189373997006335 1106 19 1.1517803393160335 1106 20 0.7086259184041808 1106 21 0.2067404407891189 1106 22 0.5261173749545023 1106 23 0.9236147075724332 1106 24 0.9343390032214636 1106 25 0.8500848185441541 1107 1 2.5340891780637165 1107 2 1.8913574899681684 1107 3 2.6622997425916672 1107 4 2.5855705556351403 1107 5 2.2571284387142647 1107 6 1.336376930231534 1107 7 0.9630728391348704 1107 8 2.8083515048785586 1107 9 4.273825652230379 1107 10 2.3738188012943056 1107 11 1.2922791200243111 1107 12 3.229802646115129 1107 13 3.4259600047133034 1107 14 -3.2216977904262087 1107 15 1.8202300097332156 1107 16 2.0702110999790335 1107 17 1.7249185142069194 1107 18 1.8101569279120717 1107 19 1.4421992594322575 1107 20 1.548174809961914 1107 21 1.181634239442225 1107 22 0.9940843890562192 1107 23 1.5456558820330244 1107 24 1.7850428516477557 1107 25 1.869424872889283 1108 1 1.9108478041403245 1108 2 1.3097266280877768 1108 3 1.5623701790773907 1108 4 1.994605534759721 1108 5 1.246077379697173 1108 6 0.6211461212682394 1108 7 0.17210836520619566 1108 8 -4.41009065063173 1108 9 -5.9791720859739 1108 10 -2.0535823077427757 1108 11 -0.2739890332663997 1108 12 1.0910489223905806 1108 13 2.322585531299101 1108 14 2.631554346383634 1108 15 -3.323040981356506 1108 16 0.19227987135375812 1108 17 0.7699488107836636 1108 18 0.6936465028506937 1108 19 0.46539800569357936 1108 20 0.4048727112759707 1108 21 0.06863076855235306 1108 22 0.18432767951519224 1108 23 0.5827501522360024 1108 24 0.341270600956765 1108 25 0.7319944184500287 1109 1 0.6035042787801999 1109 2 0.40599161777639736 1109 3 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1110 15 2.5406033890360966 1110 16 1.998550505218347 1110 17 1.259750545348595 1110 18 0.8109777576086437 1110 19 0.07539864330656343 1110 20 -0.13882036244368834 1110 21 -0.23468140547605998 1110 22 -0.4420278921273848 1110 23 -0.008655050170698786 1110 24 0.2209238876262738 1110 25 0.43896761809503104 1111 1 1.4752810812989132 1111 2 1.2875713639970279 1111 3 1.2790077968408333 1111 4 2.1602666542503925 1111 5 -1.2075967243006787 1111 6 -0.07127246755121419 1111 7 -0.529859182500136 1111 8 1.8796596591413284 1111 9 3.3893996823382864 1111 10 1.8892744184511727 1111 11 1.4039567594930205 1111 12 2.7797866123970074 1111 13 2.991016980267794 1111 14 3.1628502394306786 1111 15 3.79028211274591 1111 16 2.736481906849619 1111 17 2.169890990752677 1111 18 1.3770387245821816 1111 19 0.9833124397399615 1111 20 1.0878886540918273 1111 21 0.8012866823171763 1111 22 0.6820751341838402 1111 23 1.0616041746063303 1111 24 0.8108806298475564 1111 25 0.8918922846857598 1112 1 2.359578966399365 1112 2 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1.9701125953207566 2204 8 3.771858872448089 2204 9 -2.150076972627936 2204 10 0.7173069797903153 2204 11 1.8077523486016105 2204 12 3.767649017514632 2204 13 3.9277196302128203 2204 14 4.121127863358385 2204 15 4.631187221548291 2204 16 4.050966058332165 2204 17 3.092680400379969 2204 18 2.9899830034173114 2204 19 2.3726153519160675 2204 20 2.30205706994564 2204 21 1.8836287116094468 2204 22 1.639518439941753 2204 23 2.1327540520162387 2204 24 2.064435448490652 2204 25 2.0676497268155956 2205 1 -3.3108080938252336 2205 2 -3.8223416831076698 2205 3 -0.45488536340093333 2205 4 -2.217625147631651 2205 5 -3.0284740578643756 2205 6 -1.1277069520149752 2205 7 -0.9232630949335956 2205 8 -5.1341364796615885 2205 9 0.9278199636021576 2205 10 0.4101027497837073 2205 11 0.041750884587223425 2205 12 1.8793995096650988 2205 13 -4.534902519045577 2205 14 -0.13315153596930784 2205 15 2.2932585886960943 2205 16 1.7495205217993188 2205 17 1.5165104325740657 2205 18 0.10686678483854095 2205 19 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0.08398559824352247 2207 7 0.14085784776438715 2207 8 1.874201487408154 2207 9 -1.068740521539155 2207 10 -0.2650086413680093 2207 11 -0.15348031103291504 2207 12 -4.520591198665027 2207 13 -4.632806376395216 2207 14 -0.681413050017877 2207 15 -5.067782600986085 2207 16 -0.8606232232372619 2207 17 0.045422334477265736 2207 18 0.1633502652657839 2207 19 -0.0596938216781453 2207 20 -0.0260603273016784 2207 21 -0.1427902539720236 2207 22 -0.135450110273311 2207 23 -0.2014120200417402 2207 24 -0.38777406009033655 2207 25 0.1938789907330225 2208 1 -3.6047754168248147 2208 2 -4.315707709870281 2208 3 -1.3009584553943263 2208 4 0.047694352602459134 2208 5 -2.467735744943898 2208 6 -0.9788984550091335 2208 7 -0.5573275124894757 2208 8 -5.165394026668661 2208 9 -8.873503380984946 2208 10 -3.9938697483064374 2208 11 -1.3408509538020894 2208 12 0.32456326431652016 2208 13 1.2666426151371262 2208 14 1.3290579812686723 2208 15 1.8138677707853539 2208 16 -5.251817608760824 2208 17 -7.211693811000192 2208 18 -2.7753949761708476 2208 19 -2.2064998488118235 2208 20 -1.1469794190115365 2208 21 -1.0842794322405354 2208 22 -0.6872026468586441 2208 23 -0.4586906566128732 2208 24 -0.3991677251587866 2208 25 -0.21368491122756694 2209 1 0.8166602101184957 2209 2 0.7082932049136978 2209 3 -2.3776883946195824 2209 4 0.18881437907280585 2209 5 0.5346485324497436 2209 6 0.16859829702115192 2209 7 0.15203150616141414 2209 8 1.692639285092639 2209 9 -4.503529218766495 2209 10 -1.0442996481181304 2209 11 -0.33730373294501703 2209 12 -4.7500037947016445 2209 13 -0.01181908801547138 2209 14 1.2530871181115977 2209 15 2.1153231531699213 2209 16 -3.332530264407911 2209 17 -0.52887082656612 2209 18 0.08383290722338899 2209 19 -0.037831686700421585 2209 20 0.21980516395888117 2209 21 0.12987490349491537 2209 22 -0.05216496067821197 2209 23 0.44484585932529086 2209 24 -0.21772664843857825 2209 25 0.05739283081308244 2210 1 -0.7323427175947295 2210 2 -3.245527224657117 2210 3 -1.3486655292451921 2210 4 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2211 16 -1.653127817611498 2211 17 -6.98970487306179 2211 18 -2.936498994756114 2211 19 -1.9261177752990726 2211 20 -1.4968377678829157 2211 21 -1.4488742838073563 2211 22 -1.3249722554138827 2211 23 -1.0199962852299898 2211 24 -1.0119673701337177 2211 25 -0.7009901444862159 2212 1 1.8340830492464057 2212 2 0.9910777485781779 2212 3 1.791374102097656 2212 4 2.265438629582583 2212 5 -1.3231445609766865 2212 6 0.08875592194980442 2212 7 0.65673270803034 2212 8 -1.413726027268389 2212 9 2.887643983653348 2212 10 1.8024840948450986 2212 11 1.4815149934264156 2212 12 3.128721333661506 2212 13 3.0518856843274165 2212 14 3.312063842916647 2212 15 3.858289150212233 2212 16 2.9928383674784413 2212 17 2.2547275493876313 2212 18 1.8239043867348919 2212 19 1.3580978679299278 2212 20 1.1306807529586962 2212 21 0.8094740334618038 2212 22 0.8550536354333715 2212 23 1.0506860762382928 2212 24 1.1188571993394767 2212 25 1.4121525095521292 2213 1 2.6838258587805655 2213 2 2.123545467412992 2213 3 2.7252492630158542 2213 4 3.033102013796718 2213 5 1.9711176952775311 2213 6 -0.11328139992559372 2213 7 1.2228150535628046 2213 8 -6.205493006576123 2213 9 1.218142091645479 2213 10 1.324347374288248 2213 11 1.5384626190819333 2213 12 3.140905836473042 2213 13 -1.5287127363057742 2213 14 1.7704159848701764 2213 15 -3.4298205973900195 2213 16 0.5507730973866529 2213 17 1.7225055965234262 2213 18 1.9869988087947554 2213 19 1.7496194992363883 2213 20 2.0166517514595768 2213 21 1.4503958787685676 2213 22 1.642335803746839 2213 23 1.9778479529763544 2213 24 2.0757638639998923 2213 25 2.2787255510871347 2214 1 2.345691295448521 2214 2 1.5440381750956682 2214 3 1.0845373538125322 2214 4 1.937982491178989 2214 5 1.7238757733408043 2214 6 0.36639649734883406 2214 7 0.7058554761022044 2214 8 2.069982654774808 2214 9 3.048884835800365 2214 10 1.5736629006849228 2214 11 1.6209850654252946 2214 12 2.7535511410478613 2214 13 1.3190288903122784 2214 14 2.7241334590953024 2214 15 3.840556744798622 2214 16 2.4239103589540107 2214 17 1.7130238843266952 2214 18 1.3555398637551868 2214 19 1.1645500179066204 2214 20 0.6708739585735728 2214 21 0.6489796239191747 2214 22 0.4830135069749488 2214 23 -6.275552814993823 2214 24 -2.2326950682921094 2214 25 -0.6873361726390641 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 98374.0 3 5.0 4 726.0 5 4713.0 6 6221.0 7 6114.0 8 5396.0 9 4660.0 10 4163.0 11 4043.0 12 3781.0 13 3651.0 14 3632.0 15 3506.0 16 3376.0 17 3932.0 18 4618.0 19 6430.0 20 9117.0 21 12950.0 22 17012.0 23 21734.0 24 26499.0 25 31191.0 26 35819.0 27 40220.0 28 44316.0 29 51479.0 30 56167.0 31 61111.0 32 78774.0 33 97013.0 34 117575.0 35 134995.0 36 149160.0 37 146973.0 38 134601.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.020613680553275 17.77925581154883 13.930111204186874 23.27001930371102 2 16.00559268172023 21.88433685775695 39.10698763796539 23.00308282255743 3 16.217022629524944 27.824358271114075 31.527746236273625 24.430872863087355 4 9.93866643546551 16.85288614555045 39.20502694705401 34.003420471930035 5 11.15296136763222 39.04514796356186 35.1387254477201 14.663165221085823 6 29.516504760726576 36.639247845039975 19.96346020916025 13.880787185073201 7 28.673583857248197 32.692497212970956 21.58561858065959 17.048300349121256 8 25.080480464529188 33.09323153352503 23.81970139406283 18.006586607882962 9 25.973005654693253 15.810354589108599 23.269726771372852 34.94691298482529 10 15.46731137386797 30.037316692842168 31.82563829437761 22.669733638912245 11 33.63957868839855 24.058933301648764 23.370842273740713 18.93064573621197 12 22.500092610519296 26.524910831796234 30.985592598986667 19.989403958697803 13 27.305075327888616 22.17467123771182 27.636161580155793 22.884091854243767 14 21.988989430440302 21.375000262089856 29.073313172216796 27.562697135253046 15 22.12211498610217 28.160699022014974 26.728142806921166 22.989043184961687 16 23.518644579534225 28.302830968019695 25.56327918715029 22.615245265295798 17 22.06890623099858 28.36371464075694 26.772941835905996 22.794437292338486 18 21.954858946438506 27.709595444239742 28.077989717669226 22.257555891652526 19 23.619688711439235 27.040913668476495 27.35211836541008 21.987279254674185 20 24.255806197425745 26.565280348249487 27.177695476070785 22.001217978253983 21 25.322861787617818 26.164490025845716 26.303014122189154 22.20963406434731 22 24.613359910762462 26.357783263488372 26.188715588892276 22.840141236856894 23 23.302421677113447 26.637539629731105 27.02006788076693 23.039970812388518 24 23.387992426333938 26.844508498008384 26.73386699395491 23.033632081702777 25 22.89573208960075 26.43031926678861 27.601875104403504 23.072073539207143 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3445.0 1 3445.0 2 2533.5 3 1622.0 4 1622.0 5 1622.0 6 2184.0 7 2746.0 8 2746.0 9 2746.0 10 3059.5 11 3373.0 12 3373.0 13 3373.0 14 4620.5 15 5868.0 16 5868.0 17 5868.0 18 10255.0 19 14642.0 20 14642.0 21 14642.0 22 24259.5 23 33877.0 24 33877.0 25 33877.0 26 50066.0 27 66255.0 28 66255.0 29 66255.0 30 83169.0 31 100083.0 32 100083.0 33 100083.0 34 116938.0 35 133793.0 36 133793.0 37 133793.0 38 148287.0 39 162781.0 40 162781.0 41 162781.0 42 172788.5 43 182796.0 44 182796.0 45 182796.0 46 187130.5 47 191465.0 48 191465.0 49 191465.0 50 181933.0 51 172401.0 52 172401.0 53 172401.0 54 154421.0 55 136441.0 56 136441.0 57 136441.0 58 117254.0 59 98067.0 60 98067.0 61 98067.0 62 79865.5 63 61664.0 64 61664.0 65 61664.0 66 47291.0 67 32918.0 68 32918.0 69 32918.0 70 24835.5 71 16753.0 72 16753.0 73 16753.0 74 12066.0 75 7379.0 76 7379.0 77 7379.0 78 5376.5 79 3374.0 80 3374.0 81 3374.0 82 2391.5 83 1409.0 84 1409.0 85 1409.0 86 996.0 87 583.0 88 583.0 89 583.0 90 425.0 91 267.0 92 267.0 93 267.0 94 151.0 95 35.0 96 35.0 97 35.0 98 22.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1893940714634876 2 0.2015972977175783 3 0.20585099372614707 4 0.2674249867682161 5 0.21052308606342748 6 0.2285838609194817 7 0.2309547734488479 8 0.2276773355406064 9 0.23548740034322443 10 0.2315126352204635 11 0.22851412819802977 12 0.23179156610627127 13 0.23025744623432842 14 0.22593401750430775 15 0.22649187927592332 16 0.22516695756833632 17 0.2254458884541441 18 0.22614321566866358 19 0.2200764689023442 20 0.2655422032890135 21 0.22656161199737526 22 0.22725893921189474 23 0.23032717895578042 24 0.23011798079142454 25 0.23011798079142454 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1434047.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.91080916529255 #Duplication Level Percentage of deduplicated Percentage of total 1 89.45522808798707 66.1170829204893 2 6.101904971546392 9.019934677934305 3 1.8987399978768673 4.210122289127553 4 0.7973766832673368 2.357390234793042 5 0.4345117867880405 1.605755887668057 6 0.27736746511944144 1.2300272269862385 7 0.19253072010684158 0.9961070918591185 8 0.12852066556193645 0.7599253108915736 9 0.10331946668118194 0.6872782846439376 >10 0.5461859282487365 7.49693580881924 >50 0.044721660878347655 2.28578347606146 >100 0.018556463265093158 2.4785163902548866 >500 7.535292165304703E-4 0.401414174344809 >1k 2.8257345619892634E-4 0.3537262261265999 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 2406 0.16777692781338407 No Hit GTATCAACGCAGAGTACTTTTTTTT 1543 0.10759758920035396 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.973272145194684E-5 0.0 2 0.0 0.0 0.0 6.973272145194684E-5 0.0 3 0.0 0.0 0.0 6.973272145194684E-5 0.0 4 0.0 0.0 0.0 1.3946544290389368E-4 0.0 5 0.0 0.0 0.0 1.3946544290389368E-4 0.0 6 0.0 0.0 0.0 1.3946544290389368E-4 0.0 7 0.0 0.0 0.0 1.3946544290389368E-4 0.0 8 0.0 0.0 0.0 1.3946544290389368E-4 0.0 9 0.0 0.0 0.0 1.3946544290389368E-4 0.0 10 0.0 0.0 0.0 1.3946544290389368E-4 0.0 11 0.0 0.0 0.0 1.3946544290389368E-4 0.0 12 0.0 0.0 0.0 1.3946544290389368E-4 0.0 13 0.0 0.0 0.0 1.3946544290389368E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCGCG 280 0.0 12.556367 18 TGCGCGC 295 0.0 12.240013 19 TATCACG 70 1.0888734E-4 12.2165785 2 TTTGCGC 290 0.0 12.123814 17 CGGTTTG 90 7.437924E-6 11.614102 14 ATCAGTA 125 1.8257197E-8 11.402141 2 TAAGGCT 195 0.0 11.208407 4 ATTTGCG 300 0.0 11.086577 16 ACGGAGA 120 1.2787677E-7 11.080765 6 GACATAT 190 0.0 11.002065 1 GGACCGA 95 1.3633728E-5 10.99745 6 CGAGCAA 70 0.0014876402 10.860321 16 AATAACC 140 8.3200575E-9 10.859181 2 CGACCGT 115 8.787538E-7 10.741522 19 CCCTTAT 115 8.791485E-7 10.741146 1 AGGACCG 115 8.8391243E-7 10.736641 5 ACATGGT 710 0.0 10.707358 3 GGTATCA 1635 0.0 10.576908 1 ATCGTCT 145 1.4364559E-8 10.48546 15 AATTTGC 330 0.0 10.366307 15 >>END_MODULE