FastQCFastQC Report
Thu 26 May 2016
SRR1364232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434047
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47940.3342986666406331No Hit
TATCAACGCAGAGTACTTTTTTTTT38960.27167868277678486No Hit
GGTATCAACGCAGAGTACTTTTTTT33590.2342322113570894No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20470.14274288081213515No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18530.12921473285045748No Hit
GTGGTATCAACGCAGAGTACATGGG18020.1256583640564082No Hit
GGTATCAACGCAGAGTACATGGGAT15620.10892251090794094No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15060.10501747850663193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG551.1469261E-515.5184512
CGACCGT1009.464202E-913.35892519
ATCACGC655.548819E-513.1291653
TCGACCG956.9701855E-813.05759618
AAGTTCG1051.877197E-812.72099914
CGGTTTG1600.012.52223414
CGAAATC802.9351913E-511.84734613
TATAAGC1701.8189894E-1211.7132743
ACGCCCC907.1010236E-611.66255417
AGACGAA907.649482E-611.58487910
GACATAT1657.2759576E-1211.4939461
TCACGCA752.1100832E-411.378614
AGTTCGA1104.6905916E-711.27701515
TTCGACC1104.6905916E-711.27701517
TAATACC1803.6379788E-1211.0625364
GTATTAG1408.589268E-910.837151
GAATAAC2450.010.8371491
GGTCGGT1752.1827873E-1110.83261511
ACTCCCC2250.010.5353828
TTTATAC1553.774403E-910.3998043
Produced by FastQC (version 0.11.5)