FastQCFastQC Report
Thu 26 May 2016
SRR1364231_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364231_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324251
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31480.23771928433506942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17700.053.1107031
GTATCAA29000.045.9566351
ATCAACG33550.037.4087563
CAACGCA33850.037.2529455
TCAACGC34000.036.9136474
AACGCAG35500.035.1757286
TATCAAC36900.034.4961622
GTACATG75600.034.234631
TACATGG75700.033.5517652
ACATGGG77100.031.8624423
CCCGTAC1004.4470013E-429.7408493
ACGCAGA42850.029.2808597
CATGGGA48300.029.1866724
CGCAGAG43650.028.7442118
ATACCGA850.007232252527.9829336
TCCCGTA1150.001006698825.8616092
CATGGGG32650.024.9586324
GCAGAGT51300.023.9941379
ATGGGAT20650.023.0437585
ATGGGAG21900.022.5432935
Produced by FastQC (version 0.11.5)