FastQCFastQC Report
Thu 26 May 2016
SRR1364231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324251
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57880.43707726103284045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52060.3931278888971955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31670.23915405765221245No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14530.10972240156888685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32850.068.510641
GTATCAA59400.056.3315281
ATCAACG77150.043.496023
TCAACGC78450.042.8510864
TATCAAC78900.042.3082472
CAACGCA80000.042.090455
AACGCAG82600.041.053676
ACGCAGA93500.036.1404767
CGCAGAG94350.035.8148888
GTGGTAT15300.034.2446631
TGGTATC15400.032.4562032
TACATGG77850.031.4903682
GCAGAGT107550.031.4191939
GTACATG78800.031.3562091
ACATGGG77800.030.819943
GAGTACT63250.027.8903612-13
CAGAGTA102450.027.2634510-11
CATGGGA49100.027.143944
AGAGTAC99700.026.55351410-11
GTACTTT67150.026.00471114-15
Produced by FastQC (version 0.11.5)